The dependence of temperate phage mu-1 upon replication functions of E. coli K12

1974 ◽  
Vol 131 (3) ◽  
pp. 209-214 ◽  
Author(s):  
Ariane Toussaint ◽  
Michel Faelen
Keyword(s):  
Temperate Phage ◽  
E Coli ◽  
Phage Mu

Mapping of the modification function of temperate phage Mu-1

1980 ◽  
Vol 177 (2) ◽  
pp. 351-353 ◽  
Author(s):  
Ariane Toussaint ◽  
Lucie Desmet ◽  
Michel Faelen
Keyword(s):  
Temperate Phage ◽  
Phage Mu

scholarly journals Stable neutralization of virulent bacteria using temperate phage in the mammalian gut

2019 ◽  
Author(s):  
Bryan B. Hsu ◽  
Jeffrey C. Way ◽  
Pamela A. Silver
Keyword(s):  
Temperate Phage ◽  
Cascading Effects ◽  
E Coli ◽  
Bacterial Action ◽  
Exogenous Genes ◽  
Selection For ◽  
Mouse Model Of Infection ◽  
Bacterial Concentrations ◽  
New Framework ◽  
Lambdoid Phages

ABSTRACTElimination or alteration of select members of the gut microbiota is key to therapeutic efficacy. However, the complexity of these microbial inhabitants makes it challenging to precisely target bacteria without unexpected cascading effects. Here, we use bacteriophage to deliver exogenous genes to specific bacteria by genomic integration of temperate phage for long-lasting modification. As a real-world therapeutic test, we engineered λ phage to transcriptionally-repress shigatoxin by using genetic hybrids between λ and other lambdoid phages to overcome resistance encoded by the virulent prophage derived from enterohemorrhagic E. coli. We show that a single dose of engineered phage propagates throughout the bacterial community and reduces shigatoxin production in an enteric mouse model of infection without markedly affecting bacterial concentrations. We thus minimize the selection for resistance by relying on anti-virulence and not anti-bacterial action. Our work reveals a new framework for transferring functions to bacteria within their native environment.

scholarly journals Isolation and characterization of a novel bacteriophage, Kapi1, capable of O-antigen modification in commensal Escherichia coli.

2021 ◽  
Author(s):  
Kat Pick ◽  
Tracy Lyn Raivio
Keyword(s):  
Escherichia Coli ◽  
Molecular Mechanisms ◽  
Shigella Flexneri ◽  
Model System ◽  
Temperate Phage ◽  
Phage Displays ◽  
E Coli ◽  
O Antigen ◽  
Isolation And Characterization

In this study, we describe the isolation and characterization of novel bacteriophage Kapi1 (vB_EcoP_Kapi1) isolated from a strain of commensal Escherichia coli inhabiting the gastrointestinal tract of healthy mice. We show that Kapi1 is a temperate phage integrated into tRNA argW of strain MP1 and describe its genome annotation and structure. Kapi1 shows limited homology to other characterized prophages but is most similar to the phages of Shigella flexneri, and clusters taxonomically with P22-like phages. Investigation of the lifestyle of Kapi1 shows that this phage displays unstable lysogeny and influences the growth of its host. The receptor for Kapi1 is the lipopolysaccharide O-antigen, and we further show that Kapi1 alters the structure of its hosts O-antigen in multiple ways. We hope to use MP1 and Kapi1 as a model system to explore molecular mechanisms of mammalian colonization by E. coli and ask what the role(s) of prophages in this context might be.

Mini-Mu: An Insertion Element Derived from Temperate Phage Mu-1

1979 ◽  
Vol 43 (0) ◽  
pp. 1169-1177 ◽  
Author(s):  
M. Faelen ◽  
A. Resibois ◽  
A. Toussaint
Keyword(s):  
Temperate Phage ◽  
Insertion Element ◽  
Phage Mu

Virulent mutants of temperate phage Mu-1

1978 ◽  
Vol 160 (2) ◽  
pp. 195-202 ◽  
Author(s):  
Françoise van Vliet ◽  
Martine Couturier ◽  
Lucie Desmet ◽  
Michel Faelen ◽  
Ariane Toussaint
Keyword(s):  
Temperate Phage ◽  
Phage Mu

The DNA modification function of temperate phage Mu-1

Virology ◽  
1976 ◽  
Vol 70 (1) ◽  
pp. 17-27 ◽  
Author(s):  
Ariane Toussaint
Keyword(s):  
Temperate Phage ◽  
Dna Modification ◽  
Phage Mu

scholarly journals Identification of Site-Specific Recombination Genesint and xis of the Rhizobium Temperate Phage 16-3

1999 ◽  
Vol 181 (14) ◽  
pp. 4185-4192 ◽  
Author(s):  
Szabolcs Semsey ◽  
IstvAn Papp ◽  
Zsuzsanna Buzas ◽  
Andras Patthy ◽  
Laszlo Orosz ◽  
...  
Keyword(s):  
Dna Sequences ◽  
High Efficiency ◽  
Rhizobium Meliloti ◽  
Temperate Phage ◽  
Host Chromosome ◽  
Site Specific ◽  
Site Specific Recombination ◽  
E Coli ◽  
Tyrosine Recombinases ◽  
Recombination System

ABSTRACT Phage 16-3 is a temperate phage of Rhizobium meliloti 41 which integrates its genome with high efficiency into the host chromosome by site-specific recombination through DNA sequences of attB and attP. Here we report the identification of two phage-encoded genes required for recombinations at these sites: int (phage integration) and xis(prophage excision). We concluded that Int protein of phage16-3 belongs to the integrase family of tyrosine recombinases. Despite similarities to the cognate systems of the lambdoid phages, the 16-3 int xis att system is not active in Escherichia coli, probably due to requirements for host factors that differ in Rhizobium meliloti and E. coli. The application of the 16-3 site-specific recombination system in biotechnology is discussed.

scholarly journals Phage integration alters the respiratory strategy of its host

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Jeffrey N Carey ◽  
Erin L Mettert ◽  
Daniel R Fishman-Engel ◽  
Manuela Roggiani ◽  
Patricia J Kiley ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Laboratory Strain ◽  
Temperate Phage ◽  
Natural Isolate ◽  
Bet Hedging ◽  
Hedging Strategy ◽  
E Coli ◽  
Signal Transduction Protein ◽  
Host Genes ◽  
Host Regulation

Temperate bacteriophages are viruses that can incorporate their genomes into their bacterial hosts, existing there as prophages that refrain from killing the host cell until induced. Prophages are largely quiescent, but they can alter host phenotype through factors encoded in their genomes (often virulence factors) or by disrupting host genes as a result of integration. Here we describe another mechanism by which a prophage can modulate host phenotype. We show that a temperate phage that integrates in Escherichia coli reprograms host regulation of an anaerobic respiratory system, thereby inhibiting a bet hedging strategy. The phage exerts this effect by upregulating a host-encoded signal transduction protein through transcription initiated from a phage-encoded promoter. We further show that this phenomenon occurs not only in a laboratory strain of E. coli, but also in a natural isolate that contains a prophage at this site.

scholarly journals The properties of a temperate bacteriophage Wφ isolated from Escherichia coli strain W

1967 ◽  
Vol 9 (1) ◽  
pp. 135-139 ◽  
Author(s):  
S. W. Glover ◽  
G. Kerszman
Keyword(s):  
Escherichia Coli ◽  
Electron Microscope ◽  
Buoyant Density ◽  
Coli Strain ◽  
Temperate Phage ◽  
Temperate Bacteriophage ◽  
E Coli

Escherichia coli strain W was found to be lysogenic for a temperate phage Wφ. This phage, which plates on E. coli C, forms λ-like plaques 2–3 mm. diameter with turbid centres. It is serologically unrelated to λ but is closely related to P2 which it resembles in the electron microscope. Its buoyant density in CsCl has been measured and it is different from λ but similar to P2. E. coli C made lysogenic for Wφ restricts the growth of λ, and elsewhere (Kerszman, Glover & Aronovitch, 1967) it has been shown that the DNA of phage λ is degraded shortly after infection of bacteria lysogenic for Wφ. A mutant of Wφ has been isolated which has lost the property of restricting the growth of λ.

scholarly journals Temperate bacteriophages of Escherichia coli O119:B14

1970 ◽  
Vol 68 (3) ◽  
pp. 447-455
Author(s):  
S. S. Kasatiya
Keyword(s):  
Escherichia Coli ◽  
Indicator Strain ◽  
Temperate Phage ◽  
Biochemical Reactions ◽  
Serotype A ◽  
E Coli ◽  
Cross Reactions ◽  
Definite Relationship ◽  
Lysogenic Strain ◽  
Flagellar Antigens

SUMMARYLysogeny was detected in 98·8 % of the 343 Escherichia coli O119: B14 strains. A suitable indicator strain E. coli KS was selected to demonstrate the presence of temperate phages in this serotype. A great diversity in the temperate population was observed based on their lytic patterns and neutralization studies. No definite relationship could be established between the biochemical reactions and the flagellar antigens of the lysogenic strain and its temperate phage though some temperate phages released by E. coli O119:B14 strains with certain flagellar antigens did give specific lytic patterns and were serologically identical. Lysogenic strains, which did not release temperate phages spontaneously, were u.v. in-ductible. Cross-reactions with lysogenized colonies which were immune to corresponding phages also confirmed diversity of temperate phages in E. coli O119: B 14.

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