The uptake of copper by cell suspension cultures of Phaseolus vulgaris L. cv. SAXA

1981 ◽  
Vol 1 (1) ◽  
pp. 31-33 ◽  
Author(s):  
Werner Veltrup ◽  
Franz-Arnold Austenfeld
Keyword(s):  
Phaseolus Vulgaris ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Phaseolus Vulgaris L

scholarly journals Growth of Bauhinia forficata Link, Curcuma zedoaria Roscoe and Phaseolus vulgaris L. cell suspension cultures with carbon sources

2001 ◽  
Vol 58 (3) ◽  
pp. 481-485 ◽  
Author(s):  
Marcia Ometto Mello ◽  
Carlos Tadeu Santos Dias ◽  
Antônio Francisco Campos Amaral ◽  
Murilo Melo
Keyword(s):  
Phaseolus Vulgaris ◽  
Carbon Source ◽  
Liquid Medium ◽  
Cell Suspension ◽  
Carbon Sources ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Curcuma Zedoaria ◽  
Phaseolus Vulgaris L ◽  
Bauhinia Forficata

Cell suspension cultures of Bauhinia forficata Link, Curcuma zedoaria Roscoe and Phaseolus vulgaris L. were used to test plant ability to utilize an alternative to sucrose as carbon source and energy for growth. Glycerol, sorbitol and galactose were the alternative carbon sources tested. Cell suspension cultures established on liquid medium containing sucrose were transferred to culture medium supplemented with sucrose or glycerol, or sorbitol, or galactose as the sole carbon source. Fresh and dry weight increasing and protein content showed marked differences among the different carbon sources used. Sucrose was the best carbon source for all the three plant species tested. Galactose and glycerol promoted slow or no growth of the three studied species. Sucrose in liquid medium promoted initiation of meristemoid formation. Sorbitol, which was ineffective on promoting significant growth, was the only alternative carbon source tested that also promoted this effect.

scholarly journals 31P NMR Study of Elicitor Treated Phaseolus vulgaris Cell Suspension Cultures

1987 ◽  
Vol 85 (3) ◽  
pp. 716-719 ◽  
Author(s):  
Israel Ojalvo ◽  
J. Stefan Rokem ◽  
Gil Navon ◽  
Israel Goldberg
Keyword(s):  
Phaseolus Vulgaris ◽  
Cell Suspension ◽  
31P Nmr ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Nmr Study

The effect of 2,4-D and kinetin on the activity and isoenzyme pattern of various enzymes in cotyledon cell suspension cultures of bush bean (Phaseolus vulgaris cv. Contender)

1978 ◽  
Vol 56 (18) ◽  
pp. 2185-2195 ◽  
Author(s):  
Paul G. Arnison ◽  
W. G. Boll
Keyword(s):  
Acid Phosphatase ◽  
Phaseolus Vulgaris ◽  
Cell Suspension ◽  
Malate Dehydrogenase ◽  
Leucine Aminopeptidase ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Glutamate Oxaloacetate Transaminase ◽  
Cotyledon Cell ◽  
Isoenzyme Patterns

Changes in the activity and isoenzyme patterns of acid phosphatase, leucine aminopeptidase, glutamate-oxaloacetate transaminase, esterase, and malate and glutamate dehydrogenases were studied in cotyledon cell suspension cultures of Phaseolus vulgaris grown in the presence and absence of the growth regulators 2,4-dichlorophenoxyacetic acid and kinetin. With all enzymes studied, the pattern of isoenzymes and total enzymatic activity changed with the different phases of the culture cycle. In particular, the patterns of esterase, malate dehydrogenase, and glutamate dehydrogenase changed markedly with the inoculation of cells into fresh medium.The differences in isoenzyme patterns of cells grown with and without regulators were predominantly quantitative. However, certain minor isoenzymes of acid phosphatase, glutamate-oxaloacetate transaminase, esterase, and malate dehydrogenase were only detected in cultures grown in the presence of the regulators, while one isoenzyme of leucine aminopeptidase and two of esterase were unique to cells cultured in the absence of regulators.Three cathodic isoenzymes of acid phosphatase were released from wall material by 1 M NaCl. Such isoenzymes were also detected in the medium and in cytoplasmic extracts. Increase in the wall isoenzymes following inoculation into fresh medium was correlated with a decrease in anodic, cytoplasmic acid phosphatase.

scholarly journals Sucrose metabolizing enzymes in cell suspension cultures of Bauhinia forficata, Curcuma zedoaria and Phaseolus vulgaris

2001 ◽  
Vol 36 (9) ◽  
pp. 1085-1092 ◽  
Author(s):  
Marcia Ometto de Mello ◽  
Antônio Francisco de Campos Amaral ◽  
Murilo Melo
Keyword(s):  
Phaseolus Vulgaris ◽  
Cell Suspension ◽  
Sucrose Synthase ◽  
Sucrose Concentration ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Curcuma Zedoaria ◽  
Metabolizing Enzymes ◽  
Sucrose Metabolizing Enzymes ◽  
Bauhinia Forficata

The objective of this work was to study the activity of sucrose metabolizing enzymes in extracts of cell suspension cultures of Bauhinia forficata Link, Curcuma zedoaria Roscoe and Phaseolus vulgaris L. Invertase pathway was identified in the three studied species. Sucrose synthase pathway was also responsible for sucrose metabolism in Curcuma zedoaria and Phaseolus vulgaris cells. Activity values higher than 300 nmol min-1 mg-1 of protein were found for acid and neutral invertases, UDPglucose pyrophosphorylase and phosphoglucomutase in the cell extract of the three plant species. Sucrose synthase showed low activity in Bauhinia forficata cells. As sucrose concentration in the culture medium decreased, sucrose synthase activity increased in C. zedoaria and P. vulgaris cells. The glycolytic enzymes activity gradually reduced at the end of the culture period, when carbohydrate was limited.

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