A new type of insertion mutation in monkey cells: insertion accompanied by long target site duplication

1991 ◽  
Vol 229 (3) ◽  
pp. 325-333 ◽  
Author(s):  
Miki Ohira ◽  
Young-Seuk Bae ◽  
Hideo Ikeda
Keyword(s):  
Target Site ◽  
Target Site Duplication ◽  
Insertion Mutation ◽  
New Type

scholarly journals Emergence of CTX-M-15-Producing Enterobacteria in Cameroon and Characterization of a blaCTX-M-15-Carrying Element

2005 ◽  
Vol 49 (1) ◽  
pp. 441-443 ◽  
Author(s):  
J. Gangoue-Pieboji ◽  
V. Miriagou ◽  
S. Vourli ◽  
E. Tzelepi ◽  
P. Ngassam ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Target Site ◽  
Target Site Duplication ◽  
Truncated Form

ABSTRACT CTX-M-15-producing Klebsiella pneumoniae and Escherichia coli emerged recently in Cameroon. CTX-M-15 was encoded by two different multiresistance plasmids, of which one carried an ISEcp1-bla CTX-M-15 element flanked by a 5-bp target site duplication and inserted within a Tn2-derived sequence. A truncated form of this element in the second plasmid was identified.

scholarly journals Functional Characterization of Tn4401, a Tn3-Based Transposon Involved inblaKPCGene Mobilization

2011 ◽  
Vol 55 (11) ◽  
pp. 5370-5373 ◽  
Author(s):  
Gaelle Cuzon ◽  
Thierry Naas ◽  
Patrice Nordmann
Keyword(s):  
High Frequency ◽  
Recipient Cell ◽  
Functional Characterization ◽  
Target Site ◽  
Target Site Duplication ◽  
Site Specificity ◽  
Carbapenemase Gene

ABSTRACTThe carbapenemase geneblaKPC, which is rapidly spreading worldwide, is located on a Tn3-based transposon, Tn4401. In a transposition-conjugation assay, Tn4401was able to mobilizeblaKPC-2gene at a frequency of 4.4 × 10−6/recipient cell. A 5-bp target site duplication was evidenced upon each insertion without target site specificity. This study demonstrated that Tn4401is an active transposon capable of mobilizingblaKPCgenes at high frequency.

scholarly journals DNA Integration by Ty Integrase in yku70Mutant Saccharomyces cerevisiae Cells

2000 ◽  
Vol 20 (23) ◽  
pp. 8836-8844 ◽  
Author(s):  
Markus Kiechle ◽  
Anna A. Friedl ◽  
Palaniyandi Manivasakam ◽  
Friederike Eckardt-Schupp ◽  
Robert H. Schiestl
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Plasmid Dna ◽  
Integration Site ◽  
Cellular Function ◽  
Target Site ◽  
Target Site Duplication ◽  
Wild Type ◽  
Dna Integration ◽  
Site Preferences

ABSTRACT In the present work we examined nonhomologous integration of plasmid DNA in a yku70 mutant. Ten of 14 plasmids integrated as composite elements, including Ty sequences probably originating from erroneous strand-switching and/or priming events. Three additional plasmids integrated via Ty integrase without cointegrating Ty sequences, as inferred from 5-bp target site duplication and integration site preferences. Ty integrase-mediated integration of non-Ty DNA has never been observed in wild-type cells, although purified integrase is capable of using non-Ty DNA as a substrate in vitro. Hence our data implicate yKu70 as the cellular function preventing integrase from accepting non-Ty DNA as a substrate.

scholarly journals Evidence that chicken CR1 elements represent a novel family of retroposons.

1989 ◽  
Vol 9 (8) ◽  
pp. 3563-3566 ◽  
Author(s):  
R Silva ◽  
J B Burch
Keyword(s):  
Base Pair ◽  
Repetitive Element ◽  
Direct Repeat ◽  
Target Site ◽  
Target Site Duplication ◽  
Target Sites

We report the first precise delineation of a chicken CR1 element and show that it is flanked by a 6-base-pair target site duplication that occurred when this repetitive element transposed. The 3' end of this CR1 element is defined by an 8-base-pair imperfect direct repeat, and we infer that this sequence represents the 3' end of all intact CR1 elements. In contrast, the 5' ends are not unique, and we argue that this variation existed at the time each element transposed. We also provide evidence that CR1 elements transposed into preferred target sites. CR1 elements therefore appear to represent a novel class of passive retroposons.

scholarly journals Fidelity of Target Site Duplication and Sequence Preference during Integration of Xenotropic Murine Leukemia Virus-Related Virus

PLoS ONE ◽  
2010 ◽  
Vol 5 (4) ◽  
pp. e10255 ◽  
Author(s):  
Sanggu Kim ◽  
Alice Rusmevichientong ◽  
Beihua Dong ◽  
Roland Remenyi ◽  
Robert H. Silverman ◽  
...  
Keyword(s):  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Target Site ◽  
Target Site Duplication ◽  
Xenotropic Murine Leukemia ◽  
Sequence Preference ◽  
Related Virus ◽  
Murine Leukemia

scholarly journals Characterization of a Chlamydomonas transposon, Gulliver, resembling those in higher plants.

Genetics ◽  
1989 ◽  
Vol 122 (2) ◽  
pp. 363-377
Author(s):  
P J Ferris
Keyword(s):  
Higher Plants ◽  
Nuclear Genome ◽  
Target Site ◽  
Target Site Duplication ◽  
Genome Comparison ◽  
Higher Plant ◽  
Group Vi ◽  
Natural Isolates ◽  
A Site

Abstract While pursuing a chromosomal walk through the mt+ locus of linkage group VI of Chlamydomonas reinhardtii, I encountered a 12-kb sequence that was found to be present in approximately 12 copies in the nuclear genome. Comparison of various C. reinhardtii laboratory strains provided evidence that the sequence was mobile and therefore a transposon. One of two separate natural isolates interfertile with C. reinhardtii, C. smithii (CC-1373), contained the transposon, but at completely different locations in its nuclear genome than C. reinhardtii; and a second, CC-1952 (S1-C5), lacked the transposon altogether. Genetic analysis indicated that the transposon was found at dispersed sites throughout the genome, but had a conserved structure at each location. Sequence homology between the termini was limited to an imperfect 15-bp inverted repeat. An 8-bp target site duplication was created by insertion; transposon sequences were completely removed upon excision leaving behind both copies of the target site duplication, with minor base changes. The transposon contained an internal region of unique repetitive sequence responsible for restriction fragment length heterogeneity among the various copies of the transposon. In several cases it was possible to identify which of the dozen transposons in a given strain served as the donor when a transposition event occurred. The transposon often moved into a site genetically linked to the donor, and transposition appeared to be nonreplicative. Thus the mechanism of transposition and excision of the transposon, which I have named Gulliver, resembles that of certain higher plant transposons, like the Ac transposon of maize.

scholarly journals Precise repair of mPing excision sites is facilitated by target site duplication derived microhomology

Mobile DNA ◽  
2015 ◽  
Vol 6 (1) ◽  
Author(s):  
David M. Gilbert ◽  
M. Catherine Bridges ◽  
Ashley E. Strother ◽  
Courtney E. Burckhalter ◽  
James M. Burnette ◽  
...  
Keyword(s):  
Target Site ◽  
Target Site Duplication

Evidence that chicken CR1 elements represent a novel family of retroposons

1989 ◽  
Vol 9 (8) ◽  
pp. 3563-3566
Author(s):  
R Silva ◽  
J B Burch
Keyword(s):  
Base Pair ◽  
Repetitive Element ◽  
Direct Repeat ◽  
Target Site ◽  
Target Site Duplication ◽  
Target Sites

We report the first precise delineation of a chicken CR1 element and show that it is flanked by a 6-base-pair target site duplication that occurred when this repetitive element transposed. The 3' end of this CR1 element is defined by an 8-base-pair imperfect direct repeat, and we infer that this sequence represents the 3' end of all intact CR1 elements. In contrast, the 5' ends are not unique, and we argue that this variation existed at the time each element transposed. We also provide evidence that CR1 elements transposed into preferred target sites. CR1 elements therefore appear to represent a novel class of passive retroposons.

scholarly journals New Insertion Sequence Elements in the Upstream Region of cfiA in Imipenem-Resistant Bacteroides fragilis Strains

2003 ◽  
Vol 47 (3) ◽  
pp. 979-985 ◽  
Author(s):  
Naoki Kato ◽  
Kikuo Yamazoe ◽  
Chang-Gyun Han ◽  
Eiichi Ohtsubo
Keyword(s):  
Insertion Sequence ◽  
Bacteroides Fragilis ◽  
Target Site ◽  
Homology Search ◽  
Upstream Region ◽  
Nucleotide Sequencing ◽  
Insertion Mutation ◽  
Terminal Inverted Repeat ◽  
Loop Formation ◽  
Is Elements

ABSTRACT The 747-bp cfiA gene, which encodes a metallo-β-lactamase, and the regions flanking cfiA in six imipenem-resistant and four imipenem-susceptible Bacteroides fragilis strains isolated in Japan were analyzed by PCR and DNA sequencing. The nucleotide sequences of the cfiA genes (designated cfiA1 to cfiA10 ) of all 10 strains tested varied from that of the standard cfiA gene from B. fragilis TAL2480. However, putative proteins encoded by the cfiA variants contained conserved amino acid residues important for zinc binding and hairpin loop formation, suggesting that cfiA variants have the capability of producing metallo-β-lactamases with full catalytic activities. PCR assay indicated that six metallo-β-lactamase-producing, imipenem-resistant strains had an insertion mutation in the region immediately upstream of cfiA. Nucleotide sequencing of the PCR-amplified fragments along with the upstream region of cfiA revealed that there were five new kinds of insertion sequence (IS) elements (designated IS612, IS613, IS614, IS615, and IS616, with a size range of 1,594 to 1,691 bp), of which only IS616 was found to be almost identical to IS1188, one of the IS elements previously identified in the upstream region of cfiA. These elements had target site duplications of 4 or 5 bp in length, terminal inverted repeats (14, 15, or 17 bp in size), and a large open reading frame encoding a putative transposase which is required for the transcription of IS elements. Each element was inserted such that the transcriptional direction of the transposase was opposite to that of cfiA. A computer-aided homology search revealed that, based on the homology of their putative transposases, the sizes of their terminal inverted repeat sequences, and their target site duplications, IS612, IS613, IS614, and IS615 belong to the IS4 family, which includes IS942, previously found in some drug-resistant B. fragilis strains, but that IS616 belongs to the IS1380 family. All the IS elements appear to have putative promoter motif sequences (the −7 region's TAnnTTTG motif and the −33 region's TTG or TG) in their end regions, suggesting that the IS elements provide a promoter for the transcription of cfiA upon insertion. These data provide additional proof that various IS elements may exist to provide a promoter to express the cfiA gene.

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