Correlations between the binding of neoglycoproteins, bovine serum albumin (BSA) and lectins in 10 to 13-day-old mouse embryos

1991 ◽  
Vol 95 (3) ◽  
pp. 297-301 ◽  
Author(s):  
R. Herken ◽  
B. Sander ◽  
H. -J. Gabius ◽  
W. Götz
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Mouse Embryos

191 ALLEVIATION OF THE TWO-CELL BLOCK OF ICR, ddY, AND AKR MOUSE EMBRYOS BY BOVINE SERUM ALBUMIN

2007 ◽  
Vol 19 (1) ◽  
pp. 212
Author(s):  
K. Ono ◽  
R. Ohishi ◽  
H. Imai ◽  
M. Yamada
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Culture Media ◽  
Mouse Embryos ◽  
Control Group ◽  
Cell Block ◽  
Cell Stage ◽  
Developmental Rates

Bovine serum albumin (BSA) is an embryotrophic macromolecule used in embryo culture media and improves embryo development in vitro. However, when 1-cell embryos from some strains of mouse were cultured in traditional medium, even with BSA, developmental arrest occurred at the 2-cell stage, termed '2-cell block'. The developmental block is known to be alleviated by adding EDTA to the medium for ICR and ddY strains, and deleting phosphate from the medium for the AKR strain. Recently, our preliminary experiments revealed that the 2-cell block is relieved by adding deionized BSA (d-BSA) to the medium for the ICR strain. Thus, in the present study, we investigated whether d-BSA could rescue the embryos from ICR, ddY, and AKR strains from the 2-cell block. Fertilized 1-cell embryos were collected 20 h post-hCG from superovulated ICR, ddY, and AKR females (8-week-old) that had been mated with the ICR strain of males. Stock solutions (15%) of commercially available fraction V BSA, ovalbumin (ova), and γ-globulin (γG) were deionized over a mixed-bed ion adsorption resin. Embryos were cultured in EDTA-depleted KSOM medium with or without these deionized or non-deionized proteins at 37�C under 5% CO2 in air for 4 days. Experiments were done in at least 3 replicates, and the statistical analyses of the data were done by ANOVA and Fisher's PLDS test. To observe the distribution of BSA in the embryos from the 1-cell to the blastocyst stages, immunofluorescence study was performed using anti-BSA antibody with a laser confocal microscope. The developmental rates to the 4-cell stage of 1-cell embryos cultured in medium without (control group) or with BSA at 0.3% in ICR and 0.6% in ddY and AKR (BSA group) were very low (ICR: 10% (4/38) and 37% (17/47); ddY: 9% (7/73) and 23% (9/37); AKR: 0% (0/60) and 0% (18/30), respectively). However, when embryos were cultured with d-BSA at 0.3% in ICR and 0.6% in ddY and AKR, the rates to the 4-cell stage significantly increased (ICR: 91% (51/56), ddY: 82% (61/76), AKR: 82% (50/60) vs. control group or BSA group: P < 0.05), and development to the blastocyst stage was observed (ICR: 79% (44/56), ddY: 65% (47/76), AKR: 63% (38/60)). When ICR embryos were cultured with 0.3% deionized-ova or deionized-�G, no significant increase was observed in developmental rates to the 4-cell stage (25% (10/40) and 24% (10/42), respectively). We next examined the critical culture period for the beneficial effects of d-BSA and intracellular distribution of BSA using ddY mouse embryos. It was found that exposure to d-BSA during the late 1-cell (24 h post-hCG) and early 2-cell stages (42 h post-hCG) promoted the development beyond the 2-cell stage. The distribution of BSA in the cytoplasm of embryos at any stage was observed. Interestingly, BSA localized in the nuclei of embryos during the late 1-cell and early 2-cell stages. In conclusion, our results suggest that BSA itself has a potential to remove the 2-cell block in ICR, ddY, and AKR strains. In addition, nuclear localization of BSA may play a key role in regulating the development beyond the 2-cell stage in the mouse embryos.

Improvement of an artificial test substrate technique for evaluation of em immunocytochemical labeling

1987 ◽  
Vol 45 ◽  
pp. 762-763
Author(s):  
G. D. Gagne ◽  
M. F. Miller
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Artificial Substrate ◽  
Chemical Fixation ◽  
As If

We recently described an artificial substrate system which could be used to optimize labeling parameters in EM immunocytochemistry (ICC). The system utilizes blocks of glutaraldehyde polymerized bovine serum albumin (BSA) into which an antigen is incorporated by a soaking procedure. The resulting antigen impregnated blocks can then be fixed and embedded as if they are pieces of tissue and the effects of fixation, embedding and other parameters on the ability of incorporated antigen to be immunocyto-chemically labeled can then be assessed. In developing this system further, we discovered that the BSA substrate can also be dried and then sectioned for immunolabeling with or without prior chemical fixation and without exposing the antigen to embedding reagents. The effects of fixation and embedding protocols can thus be evaluated separately.

Stability of Prostacyclin in Human Plasma and Whole Blood: Studies on the Protective Effect of Albumin

1981 ◽  
Vol 46 (03) ◽  
pp. 645-647 ◽  
Author(s):  
M A Orchard ◽  
C Robinson
Keyword(s):  
Platelet Aggregation ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Protective Effect ◽  
Whole Blood ◽  
Bovine Serum ◽  
Fatty Acid Content ◽  
Krebs Solution ◽  
Antiaggregatory Activity ◽  
Free Plasma

SummaryThe biological half-life of prostacyclin in Krebs solution, human cell-free plasma or whole blood was measured by bracket assay on ADP-induced platelet aggregation. At 37°C, pH 7.4, plasma and blood reduced the rate of loss of antiaggregatory activity compared with Krebs solution. The protective effect of plasma was greater than that of whole blood. This effect could be partially mimicked by the addition of human or bovine serum albumin to the Krebs solution. The stabilisation afforded by human serum albumin was dependent on the fatty acid content of the albumin, although this was less important for bovine serum albumin.

RADIOIMMUNOASSAY OF OESTRONE AND OESTRADIOL IN THE PERIPHERAL PLASMA OF THE DOMESTIC FOWL IN VARIOUS PHYSIOLOGICAL STATES AND OF HYPOPHYSECTOMIZED AND OVARIECTOMIZED FOWLS

1974 ◽  
Vol 75 (1) ◽  
pp. 133-140 ◽  
Author(s):  
B. E. Senior
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Diethyl Ether ◽  
Bovine Serum ◽  
Domestic Fowl ◽  
Laying Hens ◽  
Peripheral Plasma ◽  
The Mean ◽  
Precision And Accuracy ◽  
Chicken Ovary

ABSTRACT A radioimmunoassay was developed to measure the levels of oestrone and oestradiol in 0.5–1.0 ml of domestic fowl peripheral plasma. The oestrogens were extracted with diethyl ether, chromatographed on columns of Sephadex LH-20 and assayed with an antiserum prepared against oestradiol-17β-succinyl-bovine serum albumin using a 17 h incubation at 4°C. The specificity, sensitivity, precision and accuracy of the assays were satisfactory. Oestrogen concentrations were determined in the plasma of birds in various reproductive states. In laying hens the ranges of oestrone and oestradiol were 12–190 pg/ml and 29–327 pg/ml respectively. Levels in immature birds, in adult cockerels and in an ovariectomized hen were barely detectable. The mean concentrations of oestrone and oestradiol in the plasma of four non-laying hens (55 pg/ml and 72 pg/ml respectively) and one partially ovariectomized hen (71 pg/ml and 134 pg/ml respectively) were well within the range for laying hens. It is evident that the large, yolk-filled follicles are not the only source of oestrogens in the chicken ovary.

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