A combined method for both endogenous myeloperoxidase and acid phosphatase cytochemistry as well as immunoperoxidase surface labelling discriminating human peripheral blood-derived dendritic cells and monocytes

1991 ◽  
Vol 95 (6) ◽  
pp. 573-578 ◽  
Author(s):  
J. M. S. Arkema ◽  
I. L. Schadee-Eestermans ◽  
R. H. J. Beelen ◽  
E. C. M. Hoefsmit
Keyword(s):  
Dendritic Cells ◽  
Acid Phosphatase ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Combined Method ◽  
Surface Labelling ◽  
Phosphatase Cytochemistry

scholarly journals Differential Infectivity and Division ofToxoplasma gondii in Human Peripheral Blood Leukocytes

2000 ◽  
Vol 68 (8) ◽  
pp. 4822-4826 ◽  
Author(s):  
Jacqueline Y. Channon ◽  
Rosanne M. Seguin ◽  
Lloyd H. Kasper
Keyword(s):  
Dendritic Cells ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Cell Types ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Differential Infectivity

ABSTRACT When tachyzoites were incubated with human peripheral blood leukocytes in vitro, more monocytes and dendritic cells than neutrophils or lymphocytes were infected. Although tachyzoites were able to divide in each of these cell types, monocytes and dendritic cells were more permissive to rapid tachyzoite division than neutrophils or lymphocytes.

CC Chemokine Receptors, CCR-1 and CCR-3, Are Potentially Involved in Antigen-Presenting Cell Function of Human Peripheral Blood Monocyte-Derived Dendritic Cells

Blood ◽  
1999 ◽  
Vol 93 (1) ◽  
pp. 34-42 ◽  
Author(s):  
Katsuaki Sato ◽  
Hiroshi Kawasaki ◽  
Hitomi Nagayama ◽  
Ryo Serizawa ◽  
Junji Ikeda ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Peripheral Blood ◽  
Chemokine Receptors ◽  
Human Peripheral Blood ◽  
Flow Cytometric Analysis ◽  
Peripheral Blood Monocyte ◽  
Interleukin 4 ◽  
Blood Monocyte ◽  
Cc Chemokine ◽  
Human Peripheral Blood Monocyte

Abstract We examined the potential involvement of two CC chemokine receptors (CCRs), CCR-1 and CCR-3, in the functional activation of granulocyte-macrophage colony-stimulating factor (GM-CSF) plus interleukin-4 (IL-4)–generated human peripheral blood monocyte-derived immature dendritic cells (DCs). Flow cytometric analysis showed that CCR-1, CCR-3, CCR-5, and CXC chemokine receptor (CXCR)-4 were expressed on the cell surface of monocyte-derived DCs. Treatment with a monoclonal antibody (MoAb) to either CCR-1 or CCR-3 but not MoAbs to CCR-5 and CXCR-4 abolished chemotactic migration of monocyte-derived DCs. The DCs treated with either the anti–CCR-1 MoAb or anti–CCR-3 MoAb were less efficient than untreated DCs in proliferation of allogeneic T cells (TCs) and TC-derived secretion of interferon-γ (IFN-γ). The homotypic aggregation of DCs and heterotypic aggregation of DCs with TCs were suppressed by the anti–CCR-1 MoAb or anti–CCR-3 MoAb. These results indicate that CCR-1 and CCR-3 specifically regulate interaction of TCs and DCs in the process of antigen presentation.

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