Inheritance of plastids in interspecific hybrids of blue spruce and white spruce

1989 ◽  
Vol 78 (6) ◽  
pp. 768-774 ◽  
Author(s):  
M. Stine ◽  
B. B. Sears ◽  
D. E. Keathley
1969 ◽  
Vol 47 (11) ◽  
pp. 1693-1700 ◽  
Author(s):  
James W. Hanover ◽  
Ronald C. Wilkinson

Controlled pollinations were made within and between blue spruce (Picea pungens Engelm.) and white spruce (Picea glauca (Moench) Voss) in 1967 and 1968. Interspecific hybrids were obtained and verified by comparative measurements of several traits. The cross was successful when either species was used as the female parent. Hybrid seed germinated more rapidly than that of either species and hybrid seedlings showed some heterosis for early growth. Also, hybrid seedlings had needles intermediate between, and significantly different from, the parental seedlings. Growth form of the hybrid was quite variable compared with that of either species. Further evidence for the successful crossing of blue spruce and white spruce was provided by biochemical analyses of monoterpene compounds in the parents and progeny.


1996 ◽  
Vol 26 (3) ◽  
pp. 428-432 ◽  
Author(s):  
Andrew J. David ◽  
Daniel E. Keathley

Fifteen interspecific hybrids of Serbian spruce (Piceaomorika (Panc) Purkyne) and white spruce (Piceaglauca (Moench) Voss) representing five separate crosses, including reciprocals, were used to demonstrate maternal inheritance of mitochondrial DNA. Total DNA was extracted from foliage samples of Serbian spruce (S), white spruce (W), and both S(♂) × W(♀) and W(♂) × S(♀) hybrids, digested and probed with one of two maize mitochondrial genes, ATPaseα or COXII. ATPaseα generated diagnostic Serbian and white spruce genotypes for all five enzymes tested, while COXII differentiated between the two species for four of five enzymes. Maternal inheritance was indicated in all hybrids for every diagnostic enzyme–probe combination. No paternal or nonparental bands were detected. A dilution experiment indicated that the Serbian and white spruce mitochondrial DNA restriction fragment length polymorphisms could be detected in as little as 60 and 500 ng of total DNA, respectively. It appears that the mechanism that controls the inheritance of mitochondria in Picea is still functional in wide interspecific crosses.


1975 ◽  
Vol 53 (24) ◽  
pp. 2978-2982 ◽  
Author(s):  
E. von Rudloff

The variation in the relative amounts of the terpenes of the mature and young leaves, buds, and twigs of blue spruce was investigated during a full year. As with the spruces that were investigated previously, large changes were recorded only in the buds and in the young leaves after bud burst. The variations that were recorded in the latter for santene, tricyclene, camphene, camphor, bornyl acetate, and limonene were similar to those that were found in white spruce, but those of car-β-ene, α-pinene, and β-pinene differed. Several quantitative differences in the oil of the winter buds were also recorded. The variation of β-pinene correlated negatively with that of sabinene, γ-terpinene, and terpinolene. A transient diterpene alcohol of the geranylgeraniol type was found in the buds and young twigs during spring. The seasonal variation of the terpenes of the twig oil was smaller than the twig-to-twig variation within a tree. The implications for chemosystematic studies are discussed.


1962 ◽  
Vol 94 (4) ◽  
pp. 395-408 ◽  
Author(s):  
Margaret E. P. Cumming

Gillette (3) described this species as Chermes similis in 1907. His description was of winged females from galls but he mentioned that wingless females and their egg clusters were also present in some galls, although there was no way to make certain that they were of the same species. The galls were on blue spruce from Colorado, and winged adults from these settled on spruce in cages. Patch (4) reported this species in 1909, on Norway, white, red, and black spruces from Maine. She found that the winged females settled 'on all these spruces but preferred white spruce. Wingless females with egg clusters were present in the galls and she assumed they were Chermes similis Gillette. Annand (1) placed the species in the genus Pineus, along with all species having four abdominal spiracles. He referred to the previous writers, and gave a description of a wingless adult, “probably from a gall”, included with Gillette's type material. He tentatively called this form a fundatrix. He also described a wingless female and a ‘nymph’ which Patch had found associated with galls of P. similis, as Pineus sp.


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