The fine structural localization of endogenous and exogenous peroxidase activity in human bone marrow mast cells under pathological conditions

1990 ◽  
Vol 93 (3) ◽  
Author(s):  
L.M. Escribano ◽  
E. Villa ◽  
L. Gabriel ◽  
B. Heinrichs ◽  
J. Perez de Oteyza ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mast Cells ◽  
Peroxidase Activity ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Structural Localization ◽  
Pathological Conditions

scholarly journals Enumeration of the colony-forming units–fibroblast from mouse and human bone marrow in normal and pathological conditions

2009 ◽  
Vol 2 (1) ◽  
pp. 83-94 ◽  
Author(s):  
Sergei A. Kuznetsov ◽  
Mahesh H. Mankani ◽  
Paolo Bianco ◽  
Pamela G. Robey
Keyword(s):  
Bone Marrow ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Pathological Conditions ◽  
Colony Forming Units

scholarly journals Fibroblasts induce heparin synthesis in chondroitin sulfate E containing human bone marrow-derived mast cells

Blood ◽  
1990 ◽  
Vol 76 (6) ◽  
pp. 1188-1195
Author(s):  
L Gilead ◽  
O Bibi ◽  
E Razin
Keyword(s):  
Bone Marrow ◽  
Mast Cells ◽  
Chondroitin Sulfate ◽  
Alcian Blue ◽  
Proliferation Rate ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Thymidine Uptake ◽  
Cell Monolayers ◽  
Chondroitin Sulfate E

Human bone marrow-derived mast cells (hBMMCs), differentiated in vitro in suspension culture and under the influence of human peripheral blood mononuclear cells conditioned medium (hCM), were tested for their response to recombinant human interleukin-3 (rhIL-3) and for their behavior in different microenvironments. The hBMMCs were incubated in the presence of rhIL-3 and the changes in their proliferation rate were determined. Recombinant hIL-3 induced a more than sixfold increase in 3H-thymidine uptake into the hBMMC DNA in a dose-dependent manner. Human CM used as a control for proliferation response induced a more than eightfold maximal proliferation rate increase. Rabbit anti-rhIL-3 completely inhibited hBMMC 3H-thymidine uptake induced by rhIL-3 and decreased the hCM-induced proliferation by approximately 50%. These hBMMCs were cocultured with four different mytomicin C-treated cell monolayers and assayed for phenotypic changes. After only 2 days in coculture with either embryonic mouse skin-derived fibroblasts (MESFs) or human skin-derived fibroblasts (HSFs), a marked increase in granule number and density was noted on staining with toluidine blue. Mast cells that initially stained alcian blue+/safranin- at day 0 of coculture became alcian blue+/safranin+ during the coculture period. Human BMMC proteoglycan synthesis shifted from approximately 85% chondroitin sulfate E to approximately 60% heparin within 14 to 19 days of coculture with the MESF monolayer and to approximately 50% heparin within 19 days of coculture with the HSF monolayer. None of the above- mentioned changes were noted in cocultures of hBMMCs with 3T3 cell line fibroblast monolayers or in cocultures with bovine vascular endothelium (BVE) cell monolayers. These results demonstrate microenvironmental effects exerted by the MESF and HSF monolayers on IL-3-dependent hBMMCs similar to those reported in the conversion of murine mast cell phenotype.

Ultrastructural evidence for the origin of mast cells in normal human bone marrow

1991 ◽  
Vol 38 (1) ◽  
pp. 69-71 ◽  
Author(s):  
Nagahito Saito ◽  
Nobuo Takemori ◽  
Noriko Tachibana ◽  
Naoyuki Hayashishita
Keyword(s):  
Bone Marrow ◽  
Mast Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Ultrastructural Evidence ◽  
Normal Human ◽  
Normal Human Bone Marrow

scholarly journals The CD69 early activation molecule is overexpressed in human bone marrow mast cells from adults with indolent systemic mast cell disease

1999 ◽  
Vol 106 (2) ◽  
pp. 400-405 ◽  
Author(s):  
Beatriz Díaz-Agustín ◽  
Luis Escribano ◽  
Pilar Bravo ◽  
Sonia Herrero ◽  
Rosa Nuñez ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mast Cell ◽  
Mast Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cell Disease ◽  
Early Activation ◽  
Mast Cell Disease

scholarly journals Expression of Bcl-2 by human bone marrow mast cells and its overexpression in mast cell leukemia

1999 ◽  
Vol 60 (3) ◽  
pp. 191-195 ◽  
Author(s):  
Carlos Cerver� ◽  
Luis Escribano ◽  
Jes�s F. San Miguel ◽  
Beatriz D�az-Agust�n ◽  
Pilar Bravo ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mast Cell ◽  
Mast Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cell Leukemia ◽  
Mast Cell Leukemia

scholarly journals Human bone marrow mast cells from indolent systemic mast cell disease constitutively express increased amounts of the CD63 protein on their surface

Cytometry ◽  
1998 ◽  
Vol 34 (5) ◽  
pp. 223-228 ◽  
Author(s):  
Luis Escribano ◽  
Alberto Orfao ◽  
Beatriz D�az Agust�n ◽  
Carlos Cerver� ◽  
Sonia Herrero ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mast Cell ◽  
Mast Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cell Disease ◽  
Mast Cell Disease

scholarly journals Tissue Mast Cells in Human Bone Marrow

Blood ◽  
1951 ◽  
Vol 6 (7) ◽  
pp. 614-630 ◽  
Author(s):  
ROBERT S. FADEM
Keyword(s):  
Bone Marrow ◽  
Mast Cells ◽  
Cell Growth ◽  
Inhibitory Action ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Local Cell ◽  
Marrow Cells

Abstract 1. Tissue mast cells were found in 7 of 2800 human bone marrows studied. 2. Tissue mast cells represented 1.0 per cent or more of the marrow cells in 3 of the cases, and each of the 3 exhibited some degree of marrow depression, as evidenced by anemia, leukopenia and/or thrombocytopenia. 3. The morphology and histochemistry of tissue mast cells in human bone marrow are described. 4. A relationship between tissue mast cells and marrow cellular depression is suggested based upon a possible inhibitory action of heparin upon local cell growth.

Ultrastructural characterization of basophils and mast cells derived from CD34+ human bone marrow progenitor cells

1990 ◽  
Vol 48 (3) ◽  
pp. 354-355
Author(s):  
J.P. Goff ◽  
A. S. Kirshenbaum ◽  
J. P. Albert ◽  
D. D. Metcalfe
Keyword(s):  
Bone Marrow ◽  
Mast Cell ◽  
Mast Cells ◽  
Progenitor Cells ◽  
Mononuclear Cells ◽  
Bone Marrow Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Human Mast Cell ◽  
Mouse Mast Cell

In the mouse, mast cell progenitor cells (Thyl +) have been shown to originate in the bone marrow. In humans, it has been suggested that mast cell progenitors also exist in the bone marrow, are derived from a common stem cell, and mature under cytokine influence in the tissue. Progenitor cells or CD34+ cells comprise approximately 1% of bone marrow mononuclear cells.Previously we have shown that a heterogenous population of human bone marrow cells cultured over agarose surfaces in the presence of rhIL-3 gives rise to basophils and small numbers of mast cells. Cells were identified as basophils based on characteristic morphology, metachromatic staining with Wright-Giemsa and acid toluidine blue, surface IgE as determined by fluorescence and the presence of histamine by o-Phthaldialdehyde condensation. These cells did not stain for the mast cell specific neutral protease, tryptase. Cells identified as mast cells had IgE receptors, contained histamine and were chloroacetate esterase and human mast cell tryptase positive.

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