Deconvolution of single photon counting data with a reference method and global analysis

1985 ◽  
Vol 13 (1) ◽  
Author(s):  
J.-E. L�froth
Keyword(s):  
Single Photon ◽  
Global Analysis ◽  
Photon Counting ◽  
Reference Method ◽  
Single Photon Counting ◽  
Counting Data

Picosecond Tryptophan Fluorescence of Human Blood Serum Orosomucoid

1996 ◽  
Vol 61 (5) ◽  
pp. 808-818 ◽  
Author(s):  
Martin Hof ◽  
Stefan Vajda ◽  
Vlastimil Fidler ◽  
Vladimír Karpenko
Keyword(s):  
Single Photon ◽  
Global Analysis ◽  
Photon Counting ◽  
Tryptophan Fluorescence ◽  
Fluorescence Decay ◽  
Amino Acid Residues ◽  
Counting System ◽  
Single Photon Counting ◽  
Decay Behaviour ◽  
The Individual

The state of three tryptophyl residues in human serum orosomucoid was estimated by prediction methods based on parameters characterizing their hydrophobicity either directly, or in terms of buried surfaces of the individual amino acid residues. It is shown that tryptophan 25 is the most buried, while Trp 160 is the most exposed to the solvent. Trp 122 is in this respect in an intermediate state. The fluorescence decay behaviour was determined using a picosecond single photon counting system. The multiwavelength data were analyzed using a global analysis as well as a distribution of lifetimes program. Both procedures yielded the existence of four wavelength independent lifetimes (0.22 ns, 1.0 ns, 2.5 ns, and 8.4 ns). A tentative assignment of the decay associated spectra of the four components to the three individual tryptophans is presented.

The rotational motion of n-(9-Anthroyloxy) fatty acids in phospholipid bilayer vesicles

1983 ◽  
Vol 36 (6) ◽  
pp. 1079 ◽  
Author(s):  
E Blatt ◽  
WH Sawyer ◽  
KP Ghiggino
Keyword(s):  
Phase Transition ◽  
Fatty Acids ◽  
Single Photon ◽  
Photon Counting ◽  
Site Occupancy ◽  
Phospholipid Bilayer ◽  
Time Resolved ◽  
Single Photon Counting ◽  
Counting Data ◽  
Rotational Motions

The rotational motions of a set of n-(9-anthroyloxy) fatty acids (n = 2, 6, 9, 12) in dimyristoyl-phosphatidylcholine vesicles above and below the phase transition are investigated. Above the phase transition, single site occupancy and isotropic rotation of the fluorophores allows extraction and comparison of microviscosities from steady-state and time-resolved polarization measurements. Below the phase transition double exponential fluorescence and anisotropy decays are analysed in terms of microheterogeneity of sites. Comparison with previous reports indicates the caution needed when interpreting single photon counting data in terms of hindered rotational motions and order parameters.

scholarly journals Global analysis of time correlated single photon counting FRET-FLIM data

2009 ◽  
Vol 17 (8) ◽  
pp. 6493 ◽  
Author(s):  
Hernan E. Grecco ◽  
Pedro Roda-Navarro ◽  
Peter J. Verveer
Keyword(s):  
Single Photon ◽  
Global Analysis ◽  
Photon Counting ◽  
Single Photon Counting

Processing time-correlated single photon counting data to acquire range images

1998 ◽  
Vol 145 (4) ◽  
pp. 237 ◽  
Author(s):  
M. Umasuthan ◽  
A.M. Wallace ◽  
J.S. Massa ◽  
G.S. Buller ◽  
A.C. Walker
Keyword(s):  
Processing Time ◽  
Single Photon ◽  
Photon Counting ◽  
Range Images ◽  
Single Photon Counting ◽  
Counting Data
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