The effect of gene dosage on the synthesis and stability of RNA polymerase subunits in Escherichia coli

1974 ◽  
Vol 131 (2) ◽  
pp. 173-180 ◽  
Author(s):  
Richard S. Hayward ◽  
Stuart J. Austin ◽  
John G. Scaife
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Gene Dosage ◽  
Rna Polymerase Subunits

Biosynthesis of Escherichia coli RNA polymerase subunits upon release of rifampicin inhibition

1976 ◽  
Vol 143 (3) ◽  
pp. 297-299 ◽  
Author(s):  
Frode Engbaek ◽  
Carol Gross ◽  
Richard R. Burgess
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Rna Polymerase Subunits

scholarly journals Topography of interaction of Escherichia coli RNA polymerase subunits with lac UV5 promoter

FEBS Letters ◽  
1981 ◽  
Vol 128 (1) ◽  
pp. 46-50 ◽  
Author(s):  
A. Chenchick ◽  
R. Beabealashvilli ◽  
A. Mirzabekov
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Rna Polymerase Subunits

scholarly journals Increased rrn Gene Dosage Causes Intermittent Transcription of rRNA in Escherichia coli

1999 ◽  
Vol 181 (14) ◽  
pp. 4170-4175 ◽  
Author(s):  
Justina Voulgaris ◽  
Sarah French ◽  
Richard L. Gourse ◽  
Craig Squires ◽  
Catherine L. Squires
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Gene Dosage ◽  
Transcription Termination ◽  
Elongation Rate ◽  
Multicopy Plasmid ◽  
Constant Concentration ◽  
Time Intervals ◽  
Rrna Transcription ◽  
Irregular Spacing

ABSTRACT When the number of rRNA (rrn) operons in anEscherichia coli cells is increased by adding anrrn operon on a multicopy plasmid, the rate of rRNA expression per operon is reduced to maintain a constant concentration of rRNA in the cell. We have used electron microscopy to examine rRNA transcription in cells containing a multicopy plasmid carryingrrnB. We found that there were fewer RNA polymerase molecules transcribing the rrn genes, as predicted from previous gene dosage studies. Furthermore, RNA polymerase molecules were arranged in irregularly spaced groups along the operon. No apparent pause or transcription termination sites that would account for the irregular spacing of the groups of polymerase molecules were observed. We also found that the overall transcription elongation rate was unchanged when the rrn gene dosage was increased. Our data suggest that when rrn gene dosage is increased, initiation events, or promoter-proximal elongation events, are interrupted at irregular time intervals.

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