Intersectional cytoplasmic hybrids in Nicotiana

1984 ◽  
Vol 67 (6) ◽  
pp. 499-504 ◽  
Author(s):  
D. Aviv ◽  
S. Bleichman ◽  
P. Arzee-Gonen ◽  
E. Galun
Keyword(s):  
Cytoplasmic Hybrids

Are You a Man or a Mouse?

2008 ◽  
Vol 5 (1) ◽  
Author(s):  
Janne Rothmar Herrmann
Keyword(s):  
Stem Cell ◽  
Stem Cell Research ◽  
Slippery Slope ◽  
Current Debate ◽  
Of Mice And Men ◽  
The Creation ◽  
Cytoplasmic Hybrids

AbstractAre you a man or a mouse? This expression is used to encourage someone to be brave when they are frightened of doing something. It is also an expression which bears associations to John Steinbeck's novella Of Mice and Men, the title of which is taken from Robert Burns' poem to a Mouse, which is often quoted as “the best-laid plans of mice and men often go awry”. Thus, the title's ambiguity sketches not only the issue at stake in the current debate on the creation of chimera, hybrids and cybrids (cytoplasmic hybrids) for research, but also provocatively encourages us to regard this new possibility as an important step towards fulfilling the golden promises of stem cell research while simultaneously summarizing the worry that the creation of interspecies organisms is a slippery slope towards “the apocalyptic end of humanity”.

scholarly journals The influence of mitochondria in epigenetics revealed through naturally occurring fish cybrids

2012 ◽  
Vol 58 (1) ◽  
pp. 138-145 ◽  
Author(s):  
Bernard Angers ◽  
Antoine Dallaire ◽  
Simon Vervaet ◽  
Francis Vallières ◽  
Annie Angers
Keyword(s):  
Dna Methylation ◽  
Strong Influence ◽  
Nuclear Genome ◽  
Genome Comparison ◽  
Mitochondrial Genomes ◽  
Wild Type ◽  
Ideal Model ◽  
Naturally Occurring ◽  
Cytoplasmic Hybrids ◽  
Epigenetic Processes

Abstract Epigenetic processes are important mechanisms for phenotypic changes that occur in response to the environment. As such, it is expected that the alteration of cytoplasmic composition (the immediate environment of nuclei) results in the modification of the methylome and the expression of the nuclear genome. Cytoplasmic hybrids (or cybrids) are an ideal model to study the influence of mitochondria on gene expression. In this study, we take advantage of the natural co-occurrence of two biotypes that have a similar nuclear genome type Chrosomus eos, but harbor mitochondria from different species (C. eos in wild type or C. neogaeus in cybrids) to assess the effects of mitochondria on DNA methylation profiles and protein expression of the nuclear genome. Comparison between these biotypes is particularly relevant given their recent divergence and their low level of genetic differentiation. Variations of DNA methylation assessed on tissues from different embryonic origins revealed the distinct profiles of cybrid and wild type populations. Differences are more pronounced between wild type and cybrids than between populations of a given biotype. The proteome is also more different between biotypes than within a given biotype. These results indicate a strong influence of mitochondria on the nuclear genome, which remains detectable in different genetic and environmental contexts. These changes in the methylome and proteome of cybrids are expected to reflect the adjustments imposed by the coexistence of nuclear and mitochondrial genomes from different species.

Cytoplasmic Hybrids in Penicillium notatum

1945 ◽  
Vol 72 (4) ◽  
pp. 361 ◽  
Author(s):  
Carl C. Lindegren ◽  
Henry N. Andrews
Keyword(s):  
Penicillium Notatum ◽  
Cytoplasmic Hybrids

Cytoplasmic hybrids, by fusing fish erythrophores and normal rat kidney cells

1991 ◽  
Vol 49 ◽  
pp. 266-267
Author(s):  
K.R. Porter ◽  
K.L. Anderson
Keyword(s):  
Rat Kidney ◽  
Structural Features ◽  
Cell Type ◽  
Pigment Granules ◽  
Normal Rat ◽  
Cytoplasmic Hybrids ◽  
Pigment Aggregation ◽  
General Architecture

When cultured together in the presence of PEG, these cells fuse (M1,M3) and survive in vitro for several days. This offers an opportunity to explore the capacity of one cell type (highly organized structurally) to impose it's structural features on a relatively unorganized cell type (NRK). Also, with two cells differing in several respects, one can ask questions regarding a role of the cell center in the control of pigment aggregation and dispersion, as well as the capacity of one cell type to assemble pigment granules in the cytplasm of another.First, in observations on general architecture .the hybrids have numerous microtubules (M3), but not the organized array of thousands observed in the erythrophores (M2). Furthermore, the microtubules in the hybrid are randomly oriented essentially as they are in the NRK cell (M4). There is a tendency observed in the hybrids for the pigment granules to concentrate among the microtubules and the cistemae of the ER (M3). Thus far, however, we have not succeeded consistently with epinephrine to induce aggregation, or with caffeine, dispersion.

Pseudophysiological transcomplementary activation of reconstructed oocytes as a highly efficient method used for producing nuclear-transferred pig embryos originating from transgenic foetal fibroblast cells

2012 ◽  
Vol 15 (3) ◽  
pp. 509-516 ◽  
Author(s):  
M. Samiec ◽  
M. Skrzyszowska ◽  
D. Lipiński
Keyword(s):  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Electrical Activation ◽  
Developmental Outcome ◽  
Fibroblast Cells ◽  
Cleavage Activity ◽  
New Strategy ◽  
Cytoplasmic Hybrids ◽  
Stimulation Of

Abstract The completely new strategy of pseudophysiological transcomplementary (transcytoplasmic) activation (PP-TCA) of nuclear-transferred oocytes, which had been derived from pWAPhGH-GFPBsd transfected foetal fibroblast cells, was recently applied to the somatic cell cloning of pigs. It resulted in the considerable enhancing not only the cleavage activity of cultured cloned embryos, but also their morula and blastocyst formation rates as compared to the use of standard simultaneous fusion and electrical activation of reconstituted oocytes (77% vs. 57%, 63% vs. 46% and 40% vs. 27%, respectively). Altogether, the use of cytosolic components descended from heterologous (rabbit) zygotes as the agents for stimulation of porcine clonal cytoplasmic hybrids (cybrids) turned out to be reliable and feasible strategy for the generation of transgenic blastocysts by somatic cell nuclear transfer (SCNT). Furthermore, to our knowledge, no previous study has reported the preimplantation developmental outcome of transgenic nuclear-transferred pig embryos following the PP-TCA that was developed and optimised in our laboratory.

Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusion

Nature ◽  
1979 ◽  
Vol 281 (5730) ◽  
pp. 401-403 ◽  
Author(s):  
Geneviève Belliard ◽  
Fernand Vedel ◽  
Georges Pelletier
Keyword(s):  
Nicotiana Tabacum ◽  
Protoplast Fusion ◽  
Mitochondrial Recombination ◽  
Cytoplasmic Hybrids

Plant regeneration from cytoplasmic hybrids of rice (Oryza sativa L.)

1989 ◽  
Vol 77 (3) ◽  
pp. 305-310 ◽  
Author(s):  
Z. -Q. Yang ◽  
T. Shikanai ◽  
K. Mori ◽  
Y. Yamada
Keyword(s):  
Oryza Sativa ◽  
Plant Regeneration ◽  
Oryza Sativa L ◽  
Cytoplasmic Hybrids

scholarly journals Thylakoid polypeptides analysis of intertribal cytoplasmic hybrids

1991 ◽  
Vol 7 (4) ◽  
pp. 39-45
Author(s):  
L. A. Kovler ◽  
L. R. Shlumukov ◽  
Yu. Yu. Gleba
Keyword(s):  
Thylakoid Polypeptides ◽  
Cytoplasmic Hybrids

Viable nuclear-cytoplasmic hybrids are obtained by electrofusion

Aquaculture ◽  
1990 ◽  
Vol 85 (1-4) ◽  
pp. 328
Author(s):  
Hongxi Chen ◽  
Peilin Liu ◽  
Yonglan Yi ◽  
Hanqin Liu
Keyword(s):  
Cytoplasmic Hybrids
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