Isolation of a nitrate reductase deficient mutant of Pisum sativum by means of selection for chlorate resistance

1980 ◽  
Vol 58 (1) ◽  
pp. 39-42 ◽  
Author(s):  
W. J. Feenstra ◽  
E. Jacobsen
Keyword(s):  
Pisum Sativum ◽  
Nitrate Reductase ◽  
Deficient Mutant ◽  
Chlorate Resistance ◽  
Selection For

Effect of Nitrate on Acetylene Reduction in a Nitrate Reductase Deficient Mutant of Pea (Pisum sativum L.)

1982 ◽  
Vol 105 (5) ◽  
pp. 471-474 ◽  
Author(s):  
W.J. Feenstra ◽  
E. Jacobsen ◽  
A.C.P.M. van Swaay ◽  
A.J.C. de Visser
Keyword(s):  
Pisum Sativum ◽  
Nitrate Reductase ◽  
Acetylene Reduction ◽  
Deficient Mutant ◽  
Pisum Sativum L

scholarly journals Contribution of shoots and roots to in vivo nitrate reduction in NADH-specific nitrate reductase-deficient mutant seedlings of barley (Hordeum vulgareL.)

2004 ◽  
Vol 50 (4) ◽  
pp. 527-535 ◽  
Author(s):  
Salwa Abdel-Latif ◽  
Tahei Kawachi ◽  
Hiroyuki Fujikake ◽  
Norikuni Ohtake ◽  
Takuji Ohyama ◽  
...  
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reduction ◽  
Deficient Mutant

scholarly journals The mechanism of proton translocation driven by the respiratory nitrate reductase complex of Escherichia coli

1980 ◽  
Vol 190 (1) ◽  
pp. 79-94 ◽  
Author(s):  
Robert W. Jones ◽  
Alan Lamont ◽  
Peter B. Garland
Keyword(s):  
Escherichia Coli ◽  
Nitrate Reductase ◽  
Mutant Strain ◽  
Cytoplasmic Membrane ◽  
Reductase Activity ◽  
Proton Translocation ◽  
Deficient Mutant ◽  
Benzyl Viologen ◽  
Low Concentrations ◽  
Nitrite Reductase Activity

Low concentrations (1–50μm) of ubiquinol1 were rapidly oxidized by spheroplasts of Escherichia coli derepressed for synthesis of nitrate reductase using either nitrate or oxygen as electron acceptor. Oxidation of ubiquinol1 drove an outward translocation of protons with a corrected →H+/2e− stoichiometry [Scholes & Mitchell (1970) J. Bioenerg.1, 309–323] of 1.49 when nitrate was the acceptor and 2.28 when oxygen was the acceptor. Proton translocation driven by the oxidation of added ubiquinol1 was also observed in spheroplasts from a double quinone-deficient mutant strain AN384 (ubiA−menA−), whereas a haem-deficient mutant, strain A1004a, did not oxidize ubiquinol1. Proton translocation was not observed if either the protonophore carbonyl cyanide m-chlorophenylhydrazone or the respiratory inhibitor 2-n-heptyl-4-hydroxyquinoline N-oxide was present. When spheroplasts oxidized Diquat radical (DQ+) to the oxidized species (DQ++) with nitrate as acceptor, nitrate was reduced to nitrite according to the reaction: [Formula: see text] and nitrite was further reduced in the reaction: [Formula: see text] Nitrite reductase activity (2) was inhibited by CO, leaving nitrate reductase activity (1) unaffected. Benzyl Viologen radical (BV+) is able to cross the cytoplasmic membrane and is oxidized directly by nitrate reductase to the divalent cation, BV++. In the presence of CO, this reaction consumes two protons: [Formula: see text] The consumption of these protons could not be detected by a pH electrode in the extra-cellular bulk phase of a suspension of spheroplasts unless the cytoplasmic membrane was made permeable to protons by the addition of nigericin or tetrachlorosalicylanilide. It is concluded that the protons of eqn. (3) are consumed at the cytoplasmic aspect of the cytoplasmic membrane. Diquat radical, reduced N-methylphenazonium methosulphate and its sulphonated analogue N-methylphenazonium-3-sulphonate (PMSH) and ubiquinol1 are all oxidized by nitrate reductase via a haem-dependent, endogenous quinone-independent, 2-n-heptyl-4-hydroxyquinoline N-oxide-sensitive pathway. Approximate→H+/2e− stoichiometries were zero with Diquat radical, an electron donor, 1.0 with reduced N-methylphenazonium methosulphate or its sulphonated analogue, both hydride donors, and 2.0 with ubiquinol1 (QH2), a hydrogen donor. It is concluded that the protons appearing in the medium are derived from the reductant and the observed→H+/2e− stoichiometries are accounted for by the following reactions occurring at the periplasmic aspect of the cytoplasmic membrane.: [Formula: see text]

Regressions, correlations and combining ability of some quantitative characters in peas (Pisum sativum L.)

1977 ◽  
Vol 88 (3) ◽  
pp. 759-763 ◽  
Author(s):  
B. S. Dahiya ◽  
J. S. Brar ◽  
R. K. Bajaj
Keyword(s):  
Pisum Sativum ◽  
Combining Ability ◽  
Grain Weight ◽  
Gene Effects ◽  
Ability Estimates ◽  
Quantitative Characters ◽  
Pisum Sativum L ◽  
Weight Yield ◽  
Additive Gene ◽  
Selection For

SUMMARYEight cultivars of peas (Pisum sativum L.) were evaluated for six traits in a diallel experiment using regression coefficients, correlations and combining ability estimates. Regression coefficient was high, indicating large additive effects for 100-grain weight. Yield was significantly and positively correlated with plant height, number of pods per plant and 100-grain weight, indicating that selection for either of these would result in higher yield. The best index of yield among the traits studied was number of pods per plant. Significant general combining ability (GCA) variances and specific combining ability (SCA) variances were observed. GCA variances were larger than SCA variances except for yield, suggesting predominance of additive gene effects.

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