Field trial of a compound chromosome strain for genetic control of the sheep blowfly Lucilia cuprina

1985 ◽  
Vol 70 (1) ◽  
pp. 13-21 ◽  
Author(s):  
G. G. Foster ◽  
R. H. Maddern ◽  
R. A. Helman ◽  
E. M. Reed
Keyword(s):  
Genetic Control ◽  
Field Trial ◽  
Lucilia Cuprina

scholarly journals Genetic Analysis of Field Trials of Sex-linked Translocation Strains for Genetic Control of the Australian Sheep Blowfly Lucilia cuprina (Wiedemann)

1985 ◽  
Vol 38 (3) ◽  
pp. 275 ◽  
Author(s):  
GG Foster ◽  
WG Vogt ◽  
TL Woodburn
Keyword(s):  
Genetic Analysis ◽  
Genetic Control ◽  
Poor Performance ◽  
Field Trials ◽  
Lucilia Cuprina ◽  
Native Population ◽  
Australian Sheep ◽  
Males And Females ◽  
Progeny Tests ◽  
Release Method

The results of progeny tests of males and females captured during two field trials of sex-linked translocation strains for genetic control of L. cuprina are presented. Males released as mature larvae survived to adulthood and mated with field females. However, the levels of genetic death introduced into the population were insufficient to suppress the native population. This was due partly to seasonal ineffectiveness of the release method, and partly to poor performance of the released males. On average, the mating competitiveness of the released males was only one-third that of field males, whereas their field-reared, translocation-bearing sons were fully competitive with native males.

Improved transgenic sexing strains for genetic control of the Australian sheep blow fly Lucilia cuprina using embryo-specific gene promoters

2019 ◽  
Vol 295 (2) ◽  
pp. 287-298 ◽  
Author(s):  
Ying Yan ◽  
Megan E. Williamson ◽  
Rebecca J. Davis ◽  
Anne A. Andere ◽  
Christine J. Picard ◽  
...  
Keyword(s):  
Genetic Control ◽  
Lucilia Cuprina ◽  
Specific Gene ◽  
Gene Promoters ◽  
Blow Fly ◽  
Australian Sheep

Homozygous-viable pericentric inversions for genetic control of Lucilia Cuprina

1991 ◽  
Vol 82 (6) ◽  
pp. 681-689 ◽  
Author(s):  
G. G. Foster ◽  
G. L. Weller ◽  
D. G. Bedo
Keyword(s):  
Genetic Control ◽  
Lucilia Cuprina ◽  
Pericentric Inversions

Transgenic sexing system for genetic control of the Australian sheep blow fly Lucilia cuprina

2014 ◽  
Vol 51 ◽  
pp. 80-88 ◽  
Author(s):  
Fang Li ◽  
Holly A. Wantuch ◽  
Rebecca J. Linger ◽  
Esther J. Belikoff ◽  
Maxwell J. Scott
Keyword(s):  
Genetic Control ◽  
Lucilia Cuprina ◽  
Blow Fly ◽  
Australian Sheep

Larval release method for genetic control of the Australian sheep blowfly, Lucilia cuprina (Wiedemann) (Diptera: Calliphoridae).

1978 ◽  
Vol 68 (1) ◽  
pp. 75-83 ◽  
Author(s):  
G. G. Foster ◽  
M. J. Whitten ◽  
W. G. Vogt ◽  
T. L. Woodburn ◽  
J. T. Arnold
Keyword(s):  
Genetic Control ◽  
Adult Stage ◽  
Lucilia Cuprina ◽  
Larval Release ◽  
Australian Sheep ◽  
Progeny Tests ◽  
Free Falling ◽  
Release Method ◽  
Third Instar Larvae

AbstractA method for releasing genetically altered strains of Lucilia cuprina (Wied.) as free-falling larvae from aircraft was tested. Full-fed genetically marked third-instar larvae were released from aircraft flying at a height of 150 m and speeds of 120 or 220 km/h. Progeny tests of adult flies trapped subsequently indicated that released male larvae survived to the adult stage and were competitive with wild males for females.

scholarly journals Building a transgenic sexing strain for genetic control of the Australian sheep blow fly Lucilia cuprina using two lethal effectors

BMC Genetics ◽  
2020 ◽  
Vol 21 (S2) ◽  
Author(s):  
Ying Yan ◽  
Maxwell J. Scott
Keyword(s):  
Genetic Control ◽  
Insect Pests ◽  
Ectopic Expression ◽  
Pupal Stage ◽  
Mass Rearing ◽  
Lucilia Cuprina ◽  
Hatching Rate ◽  
Pest Species ◽  
Genetic Sexing ◽  
Stage Of Development

Abstract Background The sterile insect technique (SIT) has been successfully used in many pest management programs worldwide. Some SIT programs release both sexes due to the lack of genetic sexing strains or efficient sex separation methods but sterile females are ineffective control agents. Transgenic sexing strains (TSS) using the tetracycline-off control system have been developed in a variety of insect pests, from which females die by either of two commonly used lethal effectors: overexpression of the transcription factor tetracycline transactivator (tTA) or ectopic expression of a proapoptotic gene, such as head involution defective (hid). The lethality from tTA overexpression is thought to be due to “transcriptional squelching”, while hid causes lethality by induction of apoptosis. This study aims to create and characterize a TSS of Lucilia cuprina, which is a major pest of sheep, by combining both lethal effectors in a single transgenic strain. Results Here a stable TSS of L. cuprina (DH6) that carries two lethal effectors was successfully generated, by crossing FL3#2 which carries a female-specific tTA overexpression cassette, with EF1#12 which carries a tTA-regulated LshidAla2 cassette. Females with one copy of the FL3#2 transgene are viable but up to 99.8% of homozygous females die at the pupal stage when raised on diet that lacks tetracycline. Additionally, the female lethality of FL3#2 was partially repressed by supplying tetracycline to the parental generation. With an additional LshidAla2 effector, the female lethality of DH6 is 100% dominant and cannot be repressed by maternal tetracycline. DH6 females die at the late-larval stage. Several fitness parameters important for mass rearing such as hatching rate, adult emergence and sex ratio were comparable to those of the wild type strain. Conclusions Compared to the parental FL3#2 strain, the DH6 strain shows stronger female lethality and lethality occurs at an earlier stage of development. The combination of two tTA-dependent lethal effectors could improve strain stability under mass rearing and could reduce the risk of resistance in the field if fertile males are released. Our approach could be easily adapted for other pest species for an efficient, safe and sustainable genetic control program.

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