A computer-assisted examination of the storage protein genetic variation in 841 accessions of Triticum dicoccoides

1984 ◽  
Vol 69 (1) ◽  
pp. 79-86 ◽  
Author(s):  
L. Mansur-Vergara ◽  
C. F. Konzak ◽  
Z. K. Gerechter-Amitai ◽  
A. Grama ◽  
A. Blum
1981 ◽  
Vol 59 (2) ◽  
pp. 83-88 ◽  
Author(s):  
J. W. S. Brown ◽  
Y. Ma ◽  
F. A. Bliss ◽  
T. C. Hall

2001 ◽  
Vol 21 (17) ◽  
pp. 1289-1297 ◽  
Author(s):  
B. L. Black ◽  
C. M. Parmentier-Line ◽  
L. H. Fuchigami ◽  
G. D. Coleman

1980 ◽  
Vol 191 (2) ◽  
pp. 509-516 ◽  
Author(s):  
R R Croy ◽  
J A Gatehouse ◽  
M Tyler ◽  
D Boulter

A third storage protein, distinct from legumin and vicilin, has been purified from the seeds of pea (Pisum sativum L.). This protein has been named ‘convicilin’ and is present in protein bodies isolated from pea seeds. Convicilin has a subunit mol.wt. of 71 000 and a mol.wt. in its native form of 290 000. Convicilin is antigenically dissimilar to legumin, but gives a reaction of identity with vicilin when tested against antibodies raised against both proteins. However, convicilin contains no vicilin subunits and may be clearly separated from vicilin by non-dissociating techniques. Unlike vicilin, convicilin does not interact with concanavalin A, and contains insignificant amounts of carbohydrates. Limited heterogeneity, as shown by isoelectric focusing, N-terminal analysis, and CNBr cleavage, is present in convicilin isolated from a single pea variety; genetic variation of the protein between pea lines has also been observed.


2016 ◽  
Vol 7 ◽  
Author(s):  
Swapan Kumar Tripathy ◽  
Priyadarshini Mohanty ◽  
Monalisha Jena ◽  
Gokul Bihari Dash ◽  
Kedareswar Pradhan ◽  
...  

<p><strong>Total seed storage protein profiles of 20 urdbean genotypes including the popular variety T9 were analysed by Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). 14 genotypes could be clearly identified based on genotype-specific seed protein fingerprints while rest of the test genotypes were categorized into three protein types. Dendrogram based on electrophoretic data clustered the genotypes into seven groups at 78.5% phenon level.  TU 95-1 with TU 12-25-4 revealed lowest similarity index value (0.33) followed by TU 95-1 with PU 30 and KU 96-3(SI=0.35). Clustering pattern revealed distinctly divergent group formed by TPU 95-1 and TPU 4. These may serve as a valuable source genotype in recombination breeding.   </strong></p><p><strong>Key words: </strong>Seed storage protein profiling, SDS-PAGE, Genetic variation, urdbean.<strong></strong></p>


2015 ◽  
Vol 16 (2) ◽  
pp. 320
Author(s):  
Swapan K. Tripathy ◽  
B. R. Mohapatra ◽  
P. K. Nayak ◽  
S. Pal ◽  
N. Senapati ◽  
...  

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