The pet genes of Rhodospirillum rubrum: cloning and sequencing of the genes for the cytochrome bc 1-complex

1990 ◽  
Vol 224 (3) ◽  
pp. 373-382 ◽  
Author(s):  
Christoph Majewski ◽  
Achim Trehst
1998 ◽  
Vol 180 (6) ◽  
pp. 1596-1599 ◽  
Author(s):  
Jonathan J. Robinson ◽  
Jeffrey L. Stein ◽  
Colleen M. Cavanaugh

ABSTRACT The bacterial symbiont of the hydrothermal vent tubeworm fixes carbon via the Calvin-Benson cycle and has been shown previously to express a form II ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO). The gene cbbM, which encodes this enzyme, has been cloned and sequenced. The gene has the highest identity with thecbbM gene from Rhodospirillum rubrum, and analysis of the inferred amino acid sequence reveals that all active-site residues are conserved. This is the first form II RubisCO cloned and sequenced from a chemoautotrophic symbiont and from a deep-sea organism.


2003 ◽  
Vol 179 (5) ◽  
pp. 377-380 ◽  
Author(s):  
Irma Romero ◽  
Rodolfo García-Contreras ◽  
Heliodoro Celis

1986 ◽  
Vol 9 (1-2) ◽  
pp. 181-195 ◽  
Author(s):  
R. Max Wynn ◽  
Dale F. Gaul ◽  
Won-Ki Choi ◽  
Robert W. Shaw ◽  
David B. Knaff

1998 ◽  
Vol 38 (12) ◽  
pp. 51-56 ◽  
Author(s):  
K. Henshilwood ◽  
J. Green ◽  
D. N. Lees

This study investigates human enteric virus contamination of a shellfish harvesting area. Samples were analysed over a 14-month period for Small Round Structured Viruses (SRSVs) using a previously developed nested RT-PCR. A clear seasonal difference was observed with the largest numbers of positive samples obtained during the winter period (October to March). This data concurs with the known winter association of gastroenteric illness due to oyster consumption in the UK and also with the majority of the outbreaks associated with shellfish harvested from this area during the study period. RT-PCR positive amplicons were further characterised by cloning and sequencing. Sequence analysis of the positive samples identified eleven SRSV strains, of both Genogroup I and Genogroup II, occurring throughout the study period. Many shellfish samples contained a mixture of strains with a few samples containing up to three different strains with both Genogroups represented. The observed common occurrence of strain mixtures may have implications for the role of shellfish as a vector for dissemination of SRSV strains. These results show that nested RT-PCR can identify SRSV contamination in shellfish harvesting areas. Virus monitoring of shellfish harvesting areas by specialist laboratories using RT-PCR is a possible approach to combating the transmission of SRSVs by molluscan shellfish and could potentially offer significantly enhanced levels of public health protection.


1996 ◽  
Vol 74 (9) ◽  
pp. 2285
Author(s):  
C W Emala ◽  
J Kuhl ◽  
C A Hirshman ◽  
M A Levine

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