Monosomic additions in beet (Beta vulgaris) carrying extra chromosomes of Beta procumbens

1988 ◽  
Vol 76 (4) ◽  
pp. 577-586 ◽  
Author(s):  
J. P. C. Van Geyt ◽  
M. Oléo ◽  
W. Lange ◽  
Th. S. M. De Bock
Keyword(s):  
Beta Vulgaris ◽  
Beta Procumbens ◽  
Monosomic Additions

Construction and characterization of a BAC library for the molecular dissection of a single wild beet centromere and sugar beet (Beta vulgaris) genome analysis

Genome ◽  
2001 ◽  
Vol 44 (5) ◽  
pp. 846-855 ◽  
Author(s):  
Frank Gindullis ◽  
Daryna Dechyeva ◽  
Thomas Schmidt
Keyword(s):  
Sugar Beet ◽  
Beta Vulgaris ◽  
Bac Library ◽  
Artificial Chromosome ◽  
Average Insert Size ◽  
Insert Size ◽  
Single Chromosome ◽  
Beta Procumbens ◽  
Wild Beet

We have constructed a sugar beet bacterial artificial chromosome (BAC) library of the chromosome mutant PRO1. This Beta vulgaris mutant carries a single chromosome fragment of 6-9 Mbp that is derived from the wild beet Beta procumbens and is transmitted efficiently in meiosis and mitosis. The library consists of 50 304 clones, with an average insert size of 125 kb. Filter hybridizations revealed that approximately 3.1% of the clones contain mitochondrial or chloroplast DNA. Based on a haploid genome size of 758 Mbp, the library represents eight genome equivalents. Thus, there is a greater than 99.96% probability that any sequence of the PRO1 genome can be found in the library. Approximately 0.2% of the clones hybridized with centromeric sequences of the PRO1 minichromosome. Using the identified BAC clones in fluorescence in situ hybridization experiments with PRO1 and B. procumbens chromosome spreads, their wild-beet origin and centromeric localization were demonstrated. Comparative Southern hybridization of pulsed-field separated PRO1 DNA and BAC inserts indicate that the centromeric region of the minichromosome is represented by overlapping clones in the library. Therefore, the PRO1 BAC library provides a useful tool for the characterization of a single plant centromere and is a valuable resource for sugar beet genome analysis.Key words: Beta vulgaris, BAC library, Beta procumbens minichromosome, centromere, FISH.

Monosomic additions in beet (Beta vulgaris) carrying extra chromosomes of B. procumbens

1988 ◽  
Vol 76 (5) ◽  
pp. 656-664 ◽  
Author(s):  
W. Lange ◽  
Th. S. M. De Bock ◽  
J. P. C. Van Geyt ◽  
M. Oléo
Keyword(s):  
Beta Vulgaris ◽  
Monosomic Additions

Sugar beets homozygous for resistance to beet cyst nematode (Heterodera schachtii Schm.), developed from monosomic additions of Beta procumbens to B. vulgaris

Euphytica ◽  
1988 ◽  
Vol 38 (2) ◽  
pp. 121-131 ◽  
Author(s):  
W. Heijbroek ◽  
A. J. Roelands ◽  
J. H. de Jong ◽  
C. van Hulst ◽  
A. H. L. Schoone ◽  
...  
Keyword(s):  
Cyst Nematode ◽  
Heterodera Schachtii ◽  
Sugar Beets ◽  
Beta Procumbens ◽  
Beet Cyst Nematode ◽  
Monosomic Additions

Alien chromosome fragments conditioning resistance to beet cyst nematode in diploid descendants from monosomic additions of Beta procumbens to B. vulgaris

1986 ◽  
Vol 28 (3) ◽  
pp. 439-443 ◽  
Author(s):  
J. H. de Jong ◽  
G. J. Speckmann ◽  
Th. S. M. de Bock ◽  
W. Lange ◽  
A. van Voorst
Keyword(s):  
Beta Vulgaris ◽  
Extra Chromosome ◽  
Organizer Region ◽  
Cyst Nematode ◽  
Alien Chromosome ◽  
Satellite Chromosome ◽  
Chromosome Fragment ◽  
Beta Procumbens ◽  
Beet Cyst Nematode ◽  
Monosomic Addition

A monosomic addition plant of Beta vulgaris, containing an extra chromosome of B. procumbens with a gene for resistance to the beet cyst nematode, gave rise to a diploid descendant with an additional chromosome fragment. This fragment was found to bear the gene for resistance. A study of its morphology and behaviour made it likely that it represents the short arm of the alien chromosome and may have originated by centric fission of the single addition chromosome at anaphase I or II. The fragment showed incomplete generative transmission. In part of the somatic cells the fragment was missing and occasionally more than one fragment per cell was observed. The transmission of resistance in the telosome addition material was about 10%, which is 2.5 times lower than that of the monosomic addition. The pattern of transmission can be explained by an incomplete centromere, otherwise disturbed centric activity, or chromatid cohesiveness. Comparison with other monosomic addition plants showed that the resistance gene is located on the short arm of the satellite chromosome of B. procumbens and thus is linked to the nucleoar organizer region. The long arm of this chromosome possesses genes influencing annuality and leaf shape.Key words: Beta vulgaris, Beta procumbens, beet cyst nematode, monosomic addition, centric chromosome fragment.

Plant regeneration from protoplasts of sugar beet (Beta vulgaris)

1995 ◽  
Vol 94 (2) ◽  
pp. 342-350 ◽  
Author(s):  
Steffen Lenzner ◽  
Kurt Zoglauer ◽  
Otto Schieder
Keyword(s):  
Plant Regeneration ◽  
Sugar Beet ◽  
Beta Vulgaris
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