Structural and functional analysis of the origin of conjugal transfer of the broad-host-range IneW plasmid R388 and comparison with the related IncN plasmid R46

1991 ◽  
Vol 226 (3) ◽  
pp. 473-483 ◽  
Author(s):  
Matxalen Llosa ◽  
Silvia Bolland ◽  
Fernando de la Cruz
Keyword(s):  
Functional Analysis ◽  
Host Range ◽  
Conjugal Transfer ◽  
Broad Host Range

pIJ101, a multi-copy broad host-range Streptomyces plasmid: Functional analysis and development of DNA cloning vectors

1982 ◽  
Vol 185 (2) ◽  
pp. 223-238 ◽  
Author(s):  
Tobias Kieser ◽  
David A. Hopwood ◽  
Helen M. Wright ◽  
Charles J. Thompson
Keyword(s):  
Functional Analysis ◽  
Host Range ◽  
Broad Host Range ◽  
Cloning Vectors ◽  
Dna Cloning

scholarly journals Isolation and Analysis of Donor Chromosomal Genes Whose Deficiency Is Responsible for Accelerating Bacterial and Trans-Kingdom Conjugations by IncP1 T4SS Machinery

2021 ◽  
Vol 12 ◽  
Author(s):  
Fatin Iffah Rasyiqah Mohamad Zoolkefli ◽  
Kazuki Moriguchi ◽  
Yunjae Cho ◽  
Kazuya Kiyokawa ◽  
Shinji Yamamoto ◽  
...  
Keyword(s):  
Host Range ◽  
Gene Knockout ◽  
Synergistic Effects ◽  
Conjugal Transfer ◽  
Homologous Gene ◽  
Broad Host Range ◽  
Double Knockout ◽  
E Coli ◽  
Knockout Mutants ◽  
A Genome

Conjugal transfer is a major driving force of genetic exchange in eubacteria, and the system in IncP1-type broad-host-range plasmids transfers DNA even to eukaryotes and archaea in a process known as trans-kingdom conjugation (TKC). Although conjugation factors encoded on plasmids have been extensively analyzed, those on the donor chromosome have not. To identify the potential conjugation factor(s), a genome-wide survey on a comprehensive collection of Escherichia coli gene knockout mutants (Keio collection) as donors to Saccharomyces cerevisiae recipients was performed using a conjugal transfer system mediated by the type IV secretion system (T4SS) of the IncP1α plasmid. Out of 3,884 mutants, three mutants (ΔfrmR, ΔsufA, and ΔiscA) were isolated, which showed an increase by one order of magnitude in both E. coli–E. coli and E. coli–yeast conjugations without an increase in the mRNA accumulation level for the conjugation related genes examined. The double-knockout mutants for these genes (ΔfrmRΔsufA and ΔiscAΔfrmR) did not show synergistic effects on the conjugation efficiency, suggesting that these factors affect a common step in the conjugation machinery. The three mutants demonstrated increased conjugation efficiency in IncP1β-type but not in IncN- and IncW-type broad-host-range plasmid transfers, and the homologous gene knockout mutants against the three genes in Agrobacterium tumefaciens also showed increased TKC efficiency. These results suggest the existence of a specific regulatory system in IncP1 plasmids that enables the control of conjugation efficiency in different hosts, which could be utilized for the development of donor strains as gene introduction tools into bacteria, eukaryotes, and archaea.

scholarly journals PBAD-Based Shuttle Vectors for Functional Analysis of Toxic and Highly Regulated Genes in Pseudomonas and Burkholderia spp. and Other Bacteria

2008 ◽  
Vol 74 (23) ◽  
pp. 7422-7426 ◽  
Author(s):  
Dongru Qiu ◽  
F. Heath Damron ◽  
Takehiko Mima ◽  
Herbert P. Schweizer ◽  
Hongwei D. Yu
Keyword(s):  
Functional Analysis ◽  
Host Range ◽  
Expression Vectors ◽  
Broad Host Range ◽  
Shuttle Vectors ◽  
Conditional Expression

ABSTRACT We report the construction of a series of Escherichia-Pseudomonas broad-host-range expression vectors utilizing the PBAD promoter and the araC regulator for routine cloning, conditional expression, and analysis of tightly controlled and/or toxic genes in pseudomonads.

Structural and functional analysis of nitrogenase genes from the broad-host-range Rhizobium strain ANU240

Gene ◽  
1989 ◽  
Vol 77 (1) ◽  
pp. 141-153 ◽  
Author(s):  
J. Badenoch-Jones ◽  
T.A. Holton ◽  
C.M. Morrison ◽  
K.F. Scott ◽  
J. Shine
Keyword(s):  
Functional Analysis ◽  
Host Range ◽  
Broad Host Range ◽  
Rhizobium Strain ◽  
Nitrogenase Genes
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