Molecular cloning of a glutathione S-transferase overproduced in an insecticide-resistant strain of the housefly (Musca domestica)

1991 ◽  
Vol 227 (2) ◽  
Author(s):  
Jian-ying Wang ◽  
Steve McCommas ◽  
Michael Syvanen
Keyword(s):  
Molecular Cloning ◽  
Musca Domestica ◽  
Resistant Strain ◽  
Glutathione S Transferase

The behavioural responses of three different strains of Musca domestica (Diptera: Muscidae) to Alfacron bait in the laboratory

1992 ◽  
Vol 82 (4) ◽  
pp. 471-478 ◽  
Author(s):  
Z.A. Freeman ◽  
D.B Pinniger
Keyword(s):  
Musca Domestica ◽  
Resistant Strain ◽  
Behavioural Responses ◽  
Components Of Resistance ◽  
Minute Period ◽  
Proboscis Extension ◽  
Number Of Visits ◽  
Time Spent Feeding ◽  
Different Strains ◽  
Cage Test

AbstractA study was carried out to investigate the behavioural components of resistance in the Standlake strain of Musca domestica Linnaeus. The flies were collected from a poultry unit where azamethiphos spray-on-bait (Alfacron), had been regularly used to control the fly population. A no-choice laboratory cage test was used to observe the responses of the Standlake resistant strain to baits and compare it with two other strains namely, Sparsholt resistant and Cooper susceptible. The baits used were, Alfacron, sugar and analytical grade azamethiphos, sugar alone, and a blank target as control. Only female flies were used, each strain was exposed separately to each bait over a 50 minute period and the responses were recorded with a datalogger. The responses (per fly) recorded during each test period were: the number of visits to a bait; the time spent on a bait; the number of feeds and time spent feeding on the bait defined as the number of proboscis contacts and the length of time of proboscis contact with the bait. Knockdown of flies was recorded at the end of each test. All strains showed differences in their responses to baits. The Standlake strain showed the greatest difference to responses to Alfacron, with inhibition of proboscis extension when flies landed on the bait and a lower proboscis contact when extended, resulting in fewer flies being knocked down by Alfacron than by the sugar and azamethiphos bait. This suggested that inhibition of feeding on the Alfacron bait by the Standlake strain was caused by formulation components or contaminants and not the active insecticide ingredient, azamethiphos.

Plasmodium vivax: Molecular cloning, expression and characterization of glutathione S-transferase

2007 ◽  
Vol 116 (4) ◽  
pp. 414-418 ◽  
Author(s):  
Byoung-Kuk Na ◽  
Jung-Mi Kang ◽  
Tong-Soo Kim ◽  
Woon-Mok Sohn
Keyword(s):  
Plasmodium Vivax ◽  
Molecular Cloning ◽  
Glutathione S Transferase

Mechanisms of insecticide resistance in a temephos selected Culex quinquefasciatus (Diptera: Culicidae) strain from Sri Lanka

1990 ◽  
Vol 80 (4) ◽  
pp. 453-457 ◽  
Author(s):  
H.T.R. Peiris ◽  
J. Hemingway
Keyword(s):  
Cytochrome P450 ◽  
Sri Lanka ◽  
Culex Quinquefasciatus ◽  
Resistant Strain ◽  
Resistance Mechanism ◽  
Transferase Activity ◽  
Glutathione S Transferase ◽  
Biochemical Assays ◽  
Significant Difference ◽  
Naphthyl Acetate

AbstractCulex quinquefasciatus Say from Peliyagoda, Sri Lanka, has larval resistance to temephos, malathion, fenitrothion and chlorpyrifos. Biochemical assays on individual resistant and susceptible mosquitoes of this strain showed that there was a good correlation between this resistance and increased esterase activity with both 1-and 2-naphthyl acetate, which appears to be the major resistance mechanism in this multiple organophosphate resistant strain. There was no significant difference in malaoxon, bendiocarb or propoxur sensitivity of the acetylcholinesterase from the resistant and susceptible strains, indicating that the sensitivity of the target site has not been altered. Biochemical assays on mass homogenates of the resistant and susceptible strains showed no correlation between resistance and the level of glutathione s-transferase activity, or the amount of cytochrome P450 present.

Molecular cloning and characterization of α-class glutathione S-transferase genes from the hepatopancreas of red sea bream, Pagrus major

2005 ◽  
Vol 140 (3-4) ◽  
pp. 309-320 ◽  
Author(s):  
Takafumi Konishi ◽  
Keitaro Kato ◽  
Toshiyoshi Araki ◽  
Kentaro Shiraki ◽  
Masahiro Takagi ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Red Sea ◽  
Sea Bream ◽  
Red Sea Bream ◽  
Glutathione S Transferase ◽  
Pagrus Major
Sign in / Sign up

Export Citation Format

Share Document