A class II patatin promoter is under developmental control in both transgenic potato and tobacco plants

1989 ◽  
Vol 219 (3) ◽  
pp. 390-396 ◽  
Author(s):  
Meike Köster-Töpfer ◽  
Wolf B. Frommer ◽  
Mario Rocha-Sosa ◽  
Sabine Rosahl ◽  
Jeff Schell ◽  
...  
Keyword(s):  
Transgenic Potato ◽  
Class Ii ◽  
Tobacco Plants ◽  
Developmental Control ◽  
Patatin Promoter

scholarly journals Characterization of transgenic potato (Solanum tuberosum) tubers with increased ADPglucose pyrophosphorylase*

1996 ◽  
Vol 320 (2) ◽  
pp. 487-492 ◽  
Author(s):  
Lee J. SWEETLOVE ◽  
Michael M. BURRELL ◽  
Tom ap REES
Keyword(s):  
Solanum Tuberosum ◽  
Starch Synthesis ◽  
Transgenic Potato ◽  
Soluble Starch ◽  
Inorganic Pyrophosphatase ◽  
Cell Fractionation ◽  
Intracellular Location ◽  
Adpglucose Pyrophosphorylase ◽  
Patatin Promoter ◽  
Udpglucose Pyrophosphorylase

The aim of the work described in this paper was to characterize the tubers of potato (Solanum tuberosum var. Prairie) plants that had been transformed with the Escherichiacoli ADPglucose pyrophosphorylase (EC 2.7.7.27) gene, glgC-16, under the control of a patatin promoter. Over 30 lines of transformed plants with increased ADPglucose pyrophosphorylase activity were obtained. The tubers of six of these lines were compared with those of control plants expressing the gene for β-glucuronidase. The average increase in pyrophosphorylase activity was 200%, and the highest was 400%. Western immunoblotting of tuber extracts showed that the amounts of glgC-16 protein were linearly related to the extractable activity of the ADPglucose pyrophosphorylase. Cell fractionation studies showed that the increased activity of the pyrophosphorylase in the glgC-16 tubers had a similar intracellular location, the amyloplast fraction, to that found in the control tubers. No pleiotropic changes in the maximum catalytic activities of the following enzymes could be detected in the glgC-16 tubers: sucrose synthase, fructokinase, UDPglucose pyrophosphorylase, phosphofructokinase, soluble starch synthase, starch branching enzyme, phosphoglucomutase and alkaline inorganic pyrophosphatase. The glgC-16 tubers are held to be suitable for the study of the role of ADPglucose pyrophosphorylase in the control of starch synthesis.

scholarly journals Transformation of tobacco and potato with cDNA encoding the full-length genome of Potato leafroll virus: evidence for a novel virus distribution and host effects on virus multiplication

1999 ◽  
Vol 80 (11) ◽  
pp. 2813-2822 ◽  
Author(s):  
Liliana F. Franco-Lara ◽  
Kara D. McGeachy ◽  
Uli Commandeur ◽  
Robert R. Martin ◽  
Mike A. Mayo ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Transgenic Tobacco ◽  
Transgenic Potato ◽  
Transgenic Tobacco Plants ◽  
Mesophyll Cells ◽  
Tobacco Plants ◽  
Potato Plants ◽  
The Mean ◽  
Leafroll Virus ◽  
Tissue Prints

A full-length cDNA copy of the genome of Potato leafroll virus (PLRV) was introduced into the genome of tobacco and potato plants by Agrobacterium tumefaciens-mediated transformation. Transgenic lines were obtained in which the transgene was readily detected by PCR with DNA extracted from T1 tobacco seedlings and clonally multiplied potato plants. PLRV-specific genomic and sub- genomic RNAs, coat protein antigen and virus particles were detected in transgenic plants. Aphids fed on the transgenic tobacco plants readily transmitted PLRV to test plants. Infected transgenic tobacco plants, like non-transgenic (WT) PLRV-infected plants, displayed no symptoms of the infection but transgenic plants of potato were severely stunted. In parallel tests, the mean PLRV titres in WT tobacco plants and transgenic tobacco plants were 600 and 630 ng virus/g leaf, respectively, although differences in PLRV titres among transgenic plants were much greater than those among infected WT plants. In similar tests with potato, the mean PLRV titre of WT plants was 50 ng virus/g leaf whereas higher concentrations (up to 3400 ng virus/g leaf) accumulated in transgenic potato plants. In tissue prints of stems, PLRV was detected in similar proportions of phloem cells in transgenic and infected WT plants. In transgenic tobacco and potato plants, but not in infected WT plants, a few stem epidermal cells also contained virus. From tissue prints of transgenic tobacco leaves, it was estimated that about one in 40000 mesophyll cells contained virus, but in transgenic potato, a greater proportion of mesophyll cells was infected.

A factor that regulates the class II major histocompatibility complex gene DPA is a member of a subfamily of zinc finger proteins that includes a Drosophila developmental control protein

1994 ◽  
Vol 14 (12) ◽  
pp. 8438-8450
Author(s):  
M Sugawara ◽  
T Scholl ◽  
P D Ponath ◽  
J L Strominger
Keyword(s):  
Major Histocompatibility Complex ◽  
Zinc Finger ◽  
Major Histocompatibility Complex Gene ◽  
Class Ii ◽  
Zinc Finger Proteins ◽  
Amino Acid Residues ◽  
Major Histocompatibility ◽  
Sequence Element ◽  
Developmental Control ◽  
Histocompatibility Complex

A novel DNA sequence element termed the J element involved in the regulated expression of class II major histocompatibility complex genes was recently described. To study this element and its role in class II gene regulation further, a cDNA library was screened with oligonucleotide probes containing both the S element and the nearby J element of the human DPA gene. Several DNA clones were obtained by this procedure, one of which, clone 18, is reported and characterized here. It encodes a protein predicted to contain 688 amino acid residues, including 11 zinc finger motifs of the C2H2 type in the C-terminal region, that are Krüppel-like in the conservation of the H/C link sequence connecting them. The 160 N-terminal amino acids in the nonfinger region of clone 18 are highly homologous with similar regions of several other human, mouse, and Drosophila sequences, defining a subfamily of Krüppel-like zinc finger proteins termed TAB (tramtrack [ttk]-associated box) here. One of the Drosophila sequences, ttk, is a developmental control gene, while a second does not contain a zinc finger region but encodes a structure important in oocyte development. An acidic activation domain is located between the N-terminal conserved region of clone 18 and its zinc fingers. This protein appears to require both the S and J elements, which are separated by 10 bp for optimal binding. Antisense cDNA to clone 18 inhibited the expression of a reporter construct containing the DPA promoter, indicating its functional importance in the expression of this class II gene.

Activity of the promoter of the Lhca3.St.1 gene, encoding the potato apoprotein 2 of the light-harvesting complex of Photosystem I, in transgenic potato and tobacco plants

1993 ◽  
Vol 23 (3) ◽  
pp. 605-612 ◽  
Author(s):  
Jan-Peter Nap ◽  
Martin van Spanje ◽  
Wim G. Dirkse ◽  
Gert Baarda ◽  
Ludmila Mlynarova ◽  
...  
Keyword(s):  
Photosystem I ◽  
Light Harvesting ◽  
Transgenic Potato ◽  
Gene Encoding ◽  
Tobacco Plants ◽  
Light Harvesting Complex

scholarly journals A factor that regulates the class II major histocompatibility complex gene DPA is a member of a subfamily of zinc finger proteins that includes a Drosophila developmental control protein.

1994 ◽  
Vol 14 (12) ◽  
pp. 8438-8450 ◽  
Author(s):  
M Sugawara ◽  
T Scholl ◽  
P D Ponath ◽  
J L Strominger
Keyword(s):  
Major Histocompatibility Complex ◽  
Zinc Finger ◽  
Major Histocompatibility Complex Gene ◽  
Class Ii ◽  
Zinc Finger Proteins ◽  
Amino Acid Residues ◽  
Major Histocompatibility ◽  
Sequence Element ◽  
Developmental Control ◽  
Histocompatibility Complex

A novel DNA sequence element termed the J element involved in the regulated expression of class II major histocompatibility complex genes was recently described. To study this element and its role in class II gene regulation further, a cDNA library was screened with oligonucleotide probes containing both the S element and the nearby J element of the human DPA gene. Several DNA clones were obtained by this procedure, one of which, clone 18, is reported and characterized here. It encodes a protein predicted to contain 688 amino acid residues, including 11 zinc finger motifs of the C2H2 type in the C-terminal region, that are Krüppel-like in the conservation of the H/C link sequence connecting them. The 160 N-terminal amino acids in the nonfinger region of clone 18 are highly homologous with similar regions of several other human, mouse, and Drosophila sequences, defining a subfamily of Krüppel-like zinc finger proteins termed TAB (tramtrack [ttk]-associated box) here. One of the Drosophila sequences, ttk, is a developmental control gene, while a second does not contain a zinc finger region but encodes a structure important in oocyte development. An acidic activation domain is located between the N-terminal conserved region of clone 18 and its zinc fingers. This protein appears to require both the S and J elements, which are separated by 10 bp for optimal binding. Antisense cDNA to clone 18 inhibited the expression of a reporter construct containing the DPA promoter, indicating its functional importance in the expression of this class II gene.

Characterization of two class II chitinase genes from peanut and expression studies in transgenic tobacco plants

1996 ◽  
Vol 30 (2) ◽  
pp. 351-358 ◽  
Author(s):  
Jan-Wolfhard Kellmann ◽  
Tatjana Kleinow ◽  
Kerstin Engelhardt ◽  
Christina Philipp ◽  
Dorothee Wegener ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Class Ii ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Class Ii Chitinase ◽  
Expression Studies ◽  
Chitinase Genes
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