Cytogenetics of in vitro cultured somatic cells and regenerated plants of barley (Hordeum vulgare L.)

1988 ◽  
Vol 75 (6) ◽  
pp. 905-911 ◽  
Author(s):  
A. K. Gaponenko ◽  
T. F. Petrova ◽  
A. R. Iskakov ◽  
A. A. Sozinov
Keyword(s):  
Hordeum Vulgare ◽  
Somatic Cells ◽  
Hordeum Vulgare L ◽  
Regenerated Plants

In vitro selection for improved chlorsulfuron tolerance in barley (Hordeum vulgare L.)

Euphytica ◽  
1993 ◽  
Vol 67 (1-2) ◽  
pp. 151-154 ◽  
Author(s):  
A. M. R. Baillie ◽  
B. G. Rossnagel ◽  
K. K. Kartha
Keyword(s):  
Hordeum Vulgare ◽  
In Vitro Selection ◽  
Hordeum Vulgare L ◽  
Selection For

In vitro-Selektion bei Gerste (Hordeum vulgare L.) auf Resistenz gegen Drechslera teres

1990 ◽  
Vol 129 (4) ◽  
pp. 291-302 ◽  
Author(s):  
R. Hunold ◽  
R. Krämer ◽  
R. Kunert ◽  
H. Peterka
Keyword(s):  
Hordeum Vulgare ◽  
Hordeum Vulgare L ◽  
Drechslera Teres

Evaluation of the Enhanced Regeneration System for in vitro regeneration in barley

2006 ◽  
Vol 86 (1) ◽  
pp. 63-69
Author(s):  
Seedhabadee Ganeshan ◽  
Brian J Weir ◽  
Monica Båga ◽  
Brian G Rossnagel ◽  
Ravindra N Chibbar
Keyword(s):  
Hordeum Vulgare ◽  
Embryogenic Callus ◽  
Culture Media ◽  
In Vitro Regeneration ◽  
Cold Treatment ◽  
Regeneration System ◽  
Embryogenic Calli ◽  
Hordeum Vulgare L ◽  
Best Response

A simple two-step model for evaluation of in vitro regeneration protocols is proposed based on callus induction and regeneration from immature scutella of two Canadian barley (Hordeum vulgare L.) genotypes, AC Metcalfe and SB92559 using the Enhanced Regeneration System (ERS). The number of explants producing embryogenic callus, the number of plants per embryogenic callus and the number of plants per explant were considered. Tissue culture parameters included three combinations of growth regulators, two carbon sources in culture media, and three cold treatment regimes of spikes prior to scutella isolation. Culture medium containing 5 µM 2,4-dichlorophenoxy acetic acid (2,4-D) and 0.5 µM benzyl adenine (BA) induced the highest percent of embryogenic calli and the highest number of shoots per embryogenic callus from AC Metcalfe. Medium containing 3.75 µM 2,4-D and 0.75 µM BA gave the best response for SB92559. Both genotypes produced more shoots on maltose than on sucrose medium. A 2-d treatment of spikes at 4°C resulted in best response for SB92559. Regeneration response from AC Metcalfe scutella from spikes was unaffected by being subjected to 2, 4 or 6 d of cold. Conditions resulting in best responses from both genotypes were tested on four commercial barley varieties. However, these lines showed inferior regeneration compared to SB92559 and AC Metcalfe. Key words: Hordeum vulgare, scutella, embryogenic callus, shoot production

EFFECT OF BARLEY SUPPLEMENT TO THE RATION OF DONOR ANIMALS USED IN IN VITRO DIGESTIBILITY DETERMINATION

1970 ◽  
Vol 50 (2) ◽  
pp. 265-267 ◽  
Author(s):  
F. W. CALDER
Keyword(s):  
Hordeum Vulgare ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Barley Grain ◽  
In Vitro Digestibility ◽  
Hordeum Vulgare L ◽  
Alfalfa Hay ◽  
Medicago Sativa L ◽  
Ad Libitum

Rumen fluid from two sheep was used to determine in vitro dry matter digestibilities of 25 samples each from alfalfa hay (Medicago sativa L.) and barley grain (Hordeum vulgare L.). The sheep were fed either a good quality hay ad libitum and 1 kg rolled barley per day, or only the hay ad libitum, in a switchback design. The in vitro dry matter digestibilities of both alfalfa hay and barley were lower (P < 0.01) when the donor animal was fed hay and barley than when fed only hay.

scholarly journals Transformation of barley (Hordeum vulgare L.) by Agrobacterium tumefaciens infection of in vitro cultured ovules

2006 ◽  
Vol 25 (12) ◽  
pp. 1325-1335 ◽  
Author(s):  
Inger Bæksted Holme ◽  
Henrik Brinch-Pedersen ◽  
Mette Lange ◽  
Preben Bach Holm
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Hordeum Vulgare ◽  
Hordeum Vulgare L
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