Acetylene reduction, hydrogen evolution and nodule respiration in Phaseolus vulgaris

1989 ◽  
Vol 7 (4) ◽  
Author(s):  
M. Hungria ◽  
A.P. Ruschel
Keyword(s):  
Phaseolus Vulgaris ◽  
Hydrogen Evolution ◽  
Acetylene Reduction ◽  
Nodule Respiration

SELECTION FOR CAPTAN TOLERANCE IN THE Rhizobium phaseoli-Phaseolus vulgaris L. N2-FIXING SYMBIOSIS

1986 ◽  
Vol 66 (1) ◽  
pp. 143-150 ◽  
Author(s):  
R. J. RENNIE
Keyword(s):  
Phaseolus Vulgaris ◽  
Isotope Dilution ◽  
Acetylene Reduction ◽  
Nitrogenase Activity ◽  
Field Bean ◽  
Rhizobium Phaseoli ◽  
Phaseolus Vulgaris L ◽  
Treated Seed ◽  
Environment Chamber ◽  
Symbiotic Properties

Application of the seed-applied fungicides captan, DL-Plus, Evershield, thiram and Metalaxyl reduced nodulation in the field bean cultivar Lancer (Phaseolus vulgaris L.) in the field. Captan, Evershield, B3 and Thiram also lowered the acetylene reducing activity under the same conditions. Captan, DL-Plus, and B3 resulted in significant yield reductions of field bean inoculated with commercial multi-strain rhizobial inoculant. Since captan or captan-containing fungicides were the most potent inhibitors of symbiotic N2 fixation in field bean, spontaneous mutants of Rhizobium phaseoli strains 3644 and 8215 were selected on the basis of ability to grow in microbial medium containing 100 ppm of Captan 50 W. Controlled environment chamber and field evaluations indicated that all mutants were less sensitive to commercial rates of Captan 50 W (2.0 g per kilogram seed) than either parent strain or commercial multi-strain inoculant. Inoculation of captan-treated seed with these mutants 24 h prior to seeding did not affect nitrogenase activity or yield. Assessment of the effect of captan on the N2-fixing symbiosis and the captan tolerance of R. phaseoli strains by the acetylene reduction assay or 15N isotope dilution at levels of 15N natural abundance gave similar results. The existence of mutants of R. phaseoli tolerant to seed-applied captan but unaltered in symbiotic properties makes the combined use of captan as a seed protectant and seed-applied rhizobial inoculation fully compatible. Key words: Phaseolus vulgaris L., Rhizobium phaseoli, captan, N2 fixation, 15N isotope dilution, acetylene reduction

GROWTH AND YIELD OF WHITE BEAN (Phaseolus vulgaris L.) IN RESPONSE TO NITROGEN, PHOSPHORUS AND POTASSIUM FERTILIZER AND TO INOCULATION WITH Rhizobium

1987 ◽  
Vol 67 (2) ◽  
pp. 425-432 ◽  
Author(s):  
B. R. BUTTERY ◽  
S. J. PARK ◽  
W. I. FINDLAY
Keyword(s):  
Phaseolus Vulgaris ◽  
Acetylene Reduction ◽  
Growth And Yield ◽  
First Year ◽  
Field Bean ◽  
Phaseolus Vulgaris L ◽  
Days To Maturity ◽  
White Bean ◽  
Bean Yield ◽  
Second Year

Two white bean (Phaseolus vulgaris L.) cultivars grown for 2 yr on test plots of a long-term fertilizer trial showed a significant response to nitrate in the first year and in the 2 yr combined but not in the second year on its own. Yield in the second year was about half of that in the first year, possibly because of water stress. Bean yield was lowest in the zero nitrate, about equally high for 112 and 224 kg N ha−1, but somewhat lower at 336 kg N ha−1. Annual application of potassium and phosphorus (compared to no application for 12 yr) did not affect bean yield, but increased plant dry weight after 24 d in 1984 and the 2 yr combined, after 38 d in 1984 and after 52 d in 1983 and also increased the number of days to maturity. Nitrate effects on the dry weights of samples taken during the growing season were similar to those for bean yield. Acetylene reduction rates (first year) and nodule weights were highest in zero N and declined to very low values at 336 kg N ha−1. Acetylene reduction rate per plant reached a maximum value early in the season and declined to low values before bean filling was complete. Rhizobium inoculation had no discernible effects on dry weights or yield, but a small significant effect on days to maturity. We conclude that these white bean cultivars are unable to fix sufficient nitrogen to produce maximum yield.Key words: Field bean, Phaseolus vulgaris, nitrogen, Rhizobium

Relationship Between Acetylene Reduction and Dinitrogen Fixation in Pisum sativum L. and Phaseolus vulgaris L.

1985 ◽  
Vol 140 (6) ◽  
pp. 439-447
Author(s):  
V. Našinec ◽  
V. Škrdleta ◽  
Hana Marečková ◽  
Vanda Našocová ◽  
Alena Gaudinová
Keyword(s):  
Phaseolus Vulgaris ◽  
Pisum Sativum ◽  
Acetylene Reduction ◽  
Dinitrogen Fixation ◽  
Phaseolus Vulgaris L ◽  
Pisum Sativum L
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