The effects of acyl chain ordering and crystallization on the main phase transition of wet lipid bilayers

1987 ◽  
Vol 15 (2) ◽  
Author(s):  
M.J. Zuckermann ◽  
O.G. Mouritsen
Keyword(s):  
Phase Transition ◽  
Lipid Bilayers ◽  
Acyl Chain ◽  
Main Phase ◽  
Chain Ordering

Waveguide-enhanced scattering from thin biomolecular films

2002 ◽  
Vol 35 (2) ◽  
pp. 163-167 ◽  
Author(s):  
F. Pfeiffer ◽  
U. Mennicke ◽  
T. Salditt
Keyword(s):  
Lipid Bilayers ◽  
Signal To Noise Ratio ◽  
Acyl Chain ◽  
Grazing Incidence ◽  
Waveguide Structure ◽  
X Ray Diffraction ◽  
Signal To Noise ◽  
X Ray ◽  
Grazing Incidence Diffraction ◽  
Chain Ordering

An X-ray diffraction experiment on multilamellar membranes incorporated into an X-ray waveguide structure is reported. In the device, the lipid bilayers are confined to one side by the silicon substrate and to the other side by an evaporated thin metal cap layer. Shining a highly brilliant X-ray beam onto the system, resonantly enhanced, precisely defined and clearly distinguishable standing-wavefield distributions (modes) are excited. The in-plane structure of the acyl chain ordering is then studied by grazing incidence diffraction under simultaneously excited modes. A significant gain in signal-to-noise ratio as well as enhanced spatial resolution can be obtained with such a setup.

scholarly journals The activity of the intrinsically water-soluble enzyme ADAMTS13 correlates with the membrane state when bound to a phospholipid bilayer

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Andrej Kamenac ◽  
Tobias Obser ◽  
Achim Wixforth ◽  
Matthias F. Schneider ◽  
Christoph Westerhausen
Keyword(s):  
Phase Transition ◽  
Acyl Chain ◽  
Water Soluble ◽  
Main Phase ◽  
Phospholipid Bilayer ◽  
Alternative Theory ◽  
Soluble Enzyme ◽  
Arrhenius Behavior ◽  
Activity Measurements ◽  
Gibb’S Free Energy

AbstractMembrane-associated enzymes have been found to behave differently qualitatively and quantitatively in terms of activity. These findings were highly debated in the 1970s and many general correlations and reaction specific models have been proposed, reviewed, and discarded. However, new biological applications brought up the need for clarification and elucidation. To address literature shortcomings, we chose the intrinsically water-soluble enzyme a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13 (ADAMTS13) and large unilamellar vesicles with a relative broad phase transition. We here present activity measurements of ADAMTS13 in the freely dissolved state and the membrane associated state for phosphocholine lipids with different acyl-chain lengths (13:0, 14:0 and 15:0) and thus main phase transition temperatures. While the freely dissolved enzyme shows a simple Arrhenius behavior, the activity of membrane associated ADAMTS13 in addition shows a peak. This peak temperature correlates with the main phase transition temperature of the used lipids. These findings support an alternative theory of catalysis. This theory predicts a correlation of the membrane associated activity and the heat capacity, as both are susceptibilities of the same surface Gibb’s free energy, since the enzyme is attached to the membrane.

Supported Lipid Bilayers on Mica and Silicon Oxide: Comparison of the Main Phase Transition Behavior

2010 ◽  
Vol 114 (27) ◽  
pp. 8926-8933 ◽  
Author(s):  
Heiko M. Seeger ◽  
Alessandro Di Cerbo ◽  
Andrea Alessandrini ◽  
Paolo Facci
Keyword(s):  
Phase Transition ◽  
Lipid Bilayers ◽  
Silicon Oxide ◽  
Main Phase ◽  
Supported Lipid Bilayers ◽  
Phase Transition Behavior ◽  
Transition Behavior

scholarly journals Flexible String Model Analytical Description of Main Phase Transition in Lipid Bilayers

2016 ◽  
Vol 110 (3) ◽  
pp. 73a
Author(s):  
Sergei I. Mukhin ◽  
Boris B. Kheyfets ◽  
Timur R. Galimzyanov
Keyword(s):  
Phase Transition ◽  
Lipid Bilayers ◽  
Analytical Description ◽  
String Model ◽  
Main Phase

scholarly journals Effect of Physical Parameters on the Main Phase Transition of Supported Lipid Bilayers

2009 ◽  
Vol 97 (4) ◽  
pp. 1067-1076 ◽  
Author(s):  
H.M. Seeger ◽  
G. Marino ◽  
A. Alessandrini ◽  
P. Facci
Keyword(s):  
Phase Transition ◽  
Lipid Bilayers ◽  
Main Phase ◽  
Supported Lipid Bilayers ◽  
Physical Parameters
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