Fibrinolytic activity of in vitro cultivated human bladder cell lines

1977 ◽  
Vol 5 (3) ◽  
Author(s):  
Haruo Hisazumi ◽  
Lennart Andersson ◽  
V.Peter Collins
Keyword(s):  
Cell Lines ◽  
Fibrinolytic Activity ◽  
Human Bladder ◽  
Bladder Cell

scholarly journals Cell lines produce factors that induce fetal hemoglobin in human BFUe- derived colonies

Blood ◽  
1992 ◽  
Vol 80 (10) ◽  
pp. 2650-2655
Author(s):  
P Constantoulakis ◽  
M Walmsley ◽  
R Patient ◽  
T Papayannopoulou ◽  
T Enver ◽  
...  
Keyword(s):  
Cell Lines ◽  
Leukemia Virus ◽  
Fetal Hemoglobin ◽  
Feline Leukemia Virus ◽  
In Vitro Translation ◽  
Expression Cloning ◽  
Bladder Cell ◽  
Relative Production ◽  
Established Cell

Established cell lines were screened for secretion of activities than can stimulate fetal hemoglobin (HbF) production in adult burst-forming unit-erythroid (BFUe) cultures. Conditioned media from four cell lines, a human teratocarcinoma, an osteosarcoma, a bladder cell carcinoma, and feline leukemia virus (FeLV) A-infected feline fibroblasts (FEF-A cells), consistently increased the relative production of fetal globin in BFUe-derived colonies. In vitro translation of RNA from these cells in Xenopus oocytes yielded products that increased the gamma to gamma+beta ratio in adult erythroid colonies. These results demonstrate that a variety of cell lines produce factors that stimulate the production of HbF in vitro. The genes of such factors could be isolated by expression cloning of cDNA from cell lines using the Xenopus oocyte system.

scholarly journals Escherichia coli UropathogenesisIn Vitro: Invasion, Cellular Escape, and Secondary Infection Analyzed in a Human Bladder Cell Infection Model

2012 ◽  
Vol 80 (5) ◽  
pp. 1858-1867 ◽  
Author(s):  
Thomas E. Andersen ◽  
Surabhi Khandige ◽  
Michelle Madelung ◽  
Jonathan Brewer ◽  
Hans J. Kolmos ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bacterial Colonization ◽  
Secondary Infection ◽  
Infection Model ◽  
Luminal Surface ◽  
Filamentous Bacteria ◽  
Cell Infection ◽  
Human Bladder ◽  
Bladder Cell

ABSTRACTUropathogenicEscherichia coli(UPEC) strains are capable of invading bladder epithelial cells (BECs) on the bladder luminal surface. Based primarily on studies in mouse models, invasion is proposed to trigger an intracellular uropathogenic cascade involving intracellular bacterial proliferation followed by escape of elongated, filamentous bacteria from colonized BECs. UPEC filaments on the mouse bladder epithelium are able to revert to rod-shaped bacteria, which are believed to invade neighboring cells to initiate new rounds of intracellular colonization. So far, however, these late-stage infection events have not been replicatedin vitro. We have established anin vitromodel of human bladder cell infection by the use of a flow chamber (FC)-based culture system, which allows investigation of steps subsequent to initial invasion. Short-term bacterial colonization on the FC-BEC layer led to intracellular colonization. Exposing invaded BECs to a flow of urine, i.e., establishing conditions similar to those faced by UPEC reemerging on the bladder luminal surface, led to outgrowth of filamentous bacteria similar to what has been reported to occur in mice. These filaments were capable of reverting to rods that could invade other BECs. Hence, under growth conditions established to resemble those presentin vivo, the elements of the proposed uropathogenic cascade were inducible in a human BEC model system. Here, we describe the model and show how these characteristics are reproducedin vitro.

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