scholarly journals The effect of glucose stimulation on 45calcium uptake of rat pancreatic islets and their total calcium content as measured by a fluorometric micro-method

Diabetologia ◽  
1982 ◽  
Vol 22 (2) ◽  
Author(s):  
G.H.J. Wolters ◽  
J.B. Wiegman ◽  
W. Konijnendijk
Keyword(s):  
Pancreatic Islets ◽  
Calcium Content ◽  
Total Calcium ◽  
Glucose Stimulation ◽  
Rat Pancreatic Islets ◽  
Effect Of Glucose ◽  
45Calcium Uptake

scholarly journals Effect of Glucose Adenosine on 3', 5'-Monophosphate Levels in Rat Pancreatic Islets

1975 ◽  
Vol 22 (6) ◽  
pp. 479-482 ◽  
Author(s):  
SEIJI SUZUKI ◽  
HIROSHI OKA ◽  
HIROKO YASUDA ◽  
KAMEJIRO YAMASHITA ◽  
TOSHIO KANEKO ◽  
...  
Keyword(s):  
Pancreatic Islets ◽  
Rat Pancreatic Islets ◽  
Effect Of Glucose

Effect of glucose on protein phosphorylation in rat pancreatic islets

1981 ◽  
Vol 99 (3) ◽  
pp. 987-993 ◽  
Author(s):  
Seiji Suzuki ◽  
Hiroshi Oka ◽  
Hiroko Yasuda ◽  
Masahiro Ikeda ◽  
Po Yuan Cheng ◽  
...  
Keyword(s):  
Protein Phosphorylation ◽  
Pancreatic Islets ◽  
Rat Pancreatic Islets ◽  
Effect Of Glucose

Rapid Changes in Calcium Content of Rat Pancreatic Islets in Response to Glucose

Diabetes ◽  
1981 ◽  
Vol 30 (1) ◽  
pp. 52-55 ◽  
Author(s):  
G. Ribes ◽  
E. G. Siegel ◽  
C. B. Wollheim ◽  
A. E. Renold ◽  
G. W. G. Sharp
Keyword(s):  
Pancreatic Islets ◽  
Calcium Content ◽  
Rat Pancreatic Islets ◽  
Rapid Changes

scholarly journals ATP-independent glucose stimulation of sphingosine kinase in rat pancreatic islets

2010 ◽  
Vol 51 (8) ◽  
pp. 2171-2180 ◽  
Author(s):  
L. D. Mastrandrea ◽  
S. M. Sessanna ◽  
A. Del Toro ◽  
S. G. Laychock
Keyword(s):  
Pancreatic Islets ◽  
Sphingosine Kinase ◽  
Glucose Stimulation ◽  
Rat Pancreatic Islets ◽  
Stimulation Of

Roles of GTP and phospholipase C in the potentiation of Ca2+-induced insulin secretion by glucose in rat pancreatic islets

1997 ◽  
Vol 153 (1) ◽  
pp. 61-71 ◽  
Author(s):  
J Vadakekalam ◽  
M E Rabaglia ◽  
S A Metz
Keyword(s):  
Insulin Secretion ◽  
Pancreatic Islets ◽  
Phospholipase C ◽  
Mycophenolic Acid ◽  
High Glucose ◽  
Secretory Response ◽  
Rat Pancreatic Islets ◽  
Isolated Rat Islets ◽  
Effect Of Glucose ◽  
Isolated Rat

Abstract Glucose can augment insulin secretion independently of K+ channel closure, provided cytoplasmic free Ca2+ concentration is elevated. A role for phospholipase C (PLC) in this phenomenon has been both claimed and refuted. Recently, we have shown a role for GTP in the secretory effect of glucose as well as in glucose-induced PLC activation, using islets pre-treated with GTP synthesis inhibitors such as mycophenolic acid (MPA). Therefore, in the current studies, we examined first, whether glucose augments Ca2+-induced PLC activation and second, whether GTP is required for this effect, when K+(ATP) channels are kept open using diazoxide. Isolated rat islets pre-labeled with [3H]myo-inositol were studied with or without first priming with glucose. There was a 98% greater augmentation of insulin secretion by 16·7 mm glucose (in the presence of diazoxide and 40 mm K+) in primed islets; however, the ability of high glucose to augment PLC activity bore no relationship to the secretory response. MPA markedly inhibited PLC in both conditions; however, insulin secretion was only inhibited (by 46%) in primed islets. None of these differences were attributable to alterations in labeling of phosphoinositides or levels of GTP or ATP. These data indicate that an adequate level of GTP is critical for glucose's potentiation of Ca2+-induced insulin secretion in primed islets but that PLC activation can clearly be dissociated from insulin secretion and therefore cannot be the major cause of glucose's augmentation of Ca2+-induced insulin secretion. Journal of Endocrinology (1997) 153, 61–71

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