scholarly journals Effects of prednisolone and dexamethasone in vivo and in vitro: Studies of insulin binding, deoxyglucose uptake and glucose oxidation in rat adipocytes

Diabetologia ◽  
1981 ◽  
Vol 21 (2) ◽  
pp. 149-153 ◽  
Author(s):  
R. De Pirro ◽  
A. Green ◽  
M. Yung-Chin Kao ◽  
J. M. Olefsky
Keyword(s):  
Glucose Oxidation ◽  
Insulin Binding ◽  
In Vitro Studies ◽  
Rat Adipocytes

scholarly journals In vivo and in vitro effect of p-chlorophenoxyisobutyrate on insulin binding and glucose transport in isolated rat adipocytes.

1985 ◽  
Vol 32 (6) ◽  
pp. 829-836
Author(s):  
NOBUAKI WATANABE ◽  
MASASHI KOBAYASHI ◽  
HIROSHI MAEGAWA ◽  
OSAMU ISHIBASHI ◽  
YASUMITSU TAKATA ◽  
...  
Keyword(s):  
Glucose Transport ◽  
Insulin Binding ◽  
Rat Adipocytes ◽  
Vitro Effect ◽  
Isolated Rat

Receptor and postreceptor insulin resistance induced by in vivo hyperinsuiinemia

1983 ◽  
Vol 61 (8) ◽  
pp. 802-807 ◽  
Author(s):  
C. Martin ◽  
K. S. Desai ◽  
G. Steiner
Keyword(s):  
Insulin Resistance ◽  
Glucose Oxidation ◽  
Binding Capacity ◽  
Insulin Receptors ◽  
Insulin Binding ◽  
Insulin Concentration ◽  
Fat Cell ◽  
And Control

We examined the effects of inducing hyperinsuiinemia in vivo in rats on the insulin receptors of, and the glucose oxidation by their adipocytes. Hyperinsulinemia was induced over a 2-week period by injecting NPH insulin subcutaneously. This was given in doses that were gradually increased to a final dose of 6 units/day. Profound hypoglycemia was avoided by providing supplemental sucrose to both the insulin-treated and control rats. The insulin concentration was eight times greater in the insulin-treated rats. However, they were not grossly obese and their adipocytes were not enlarged. The adipocytes of the hyperinsulinemic rats had a 25% lower maximal insulin binding capacity and were resistant to the effects of insulin on glucose oxidation. We felt that the hyperinsuiinemia was sufficient so that, despite their somewhat lower insulin binding capacity, these adipocytes would not bind less insulin in vivo than would adipocytes from control rats. Hence, we postulated that, this massive hyperinsulinemia not only down regulated the insulin receptor, but also led to a postreceptor resistance. This notion was supported by two lines of in vitro data. First, even at maximally effective medium concentrations of insulin, the maximum rate of glucose oxidation by the adipocytes from hyperinsulinemic rats reached a plateau which was less than that reached by cells from controls. Second, when this in vitro glucose oxidation was related not merely to the medium insulin concentration, but to the amount of insulin bound to adipocytes, the response of the hyperinsulinemic rats' cells was always lower than control. These changes occurred in the absence of any difference in fat cell size. Thus, in vivo hyperinsulinemia led to insulin resistance in adipocytes. This was associated both with down regulation of the insulin receptors and with a postreceptor defect.

The Venom of the Boomslang (Dispholidus Typus): In Vivo and in Vitro Studies

1969 ◽  
Vol 21 (02) ◽  
pp. 234-244 ◽  
Author(s):  
N Mackay ◽  
J.C Ferguson ◽  
Antonia Bagshawe ◽  
A.T.T Forrester ◽  
G.P Mcnicol
Keyword(s):  
In Vitro Studies

SummaryAn account is given of the effects of boomslang venom in man. Evidence was found of a fibrinolytic state apparently secondary to the coagulant action of the venom. These features rapidly responded to the administration of specific antivenom. In vitro studies, using a homogenate of boomslang parotids, confirmed the coagulant properties of the venom and showed them to be of much greater potency than the proteolytic actions.

Effect of X-irradiation on gene expression of rat liver chemokines: in vivo and in vitro studies

2008 ◽  
Vol 46 (01) ◽  
Author(s):  
F Moriconi ◽  
H Christiansen ◽  
H Christiansen ◽  
N Sheikh ◽  
J Dudas ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rat Liver ◽  
In Vitro Studies ◽  
X Irradiation

Ciamexon in low-dose streptozotocin induced diabetes mellitus. In vivo and in vitro studies

1986 ◽  
Vol 113 (1_Suppl) ◽  
pp. S120-S121
Author(s):  
TH. LINN ◽  
H. GERMANN ◽  
B. HERING ◽  
R. BRETZEL ◽  
K. FEDERLIN
Keyword(s):  
Diabetes Mellitus ◽  
Low Dose ◽  
In Vitro Studies ◽  
Streptozotocin Induced Diabetes

Delayed hypersensitivity to tetanus toxoid in man: in vivo and in vitro studies

Pathology ◽  
1983 ◽  
Vol 15 (4) ◽  
pp. 369-372 ◽  
Author(s):  
Christine Johnson ◽  
R.S. Walls ◽  
A. Ruwoldt
Keyword(s):  
Tetanus Toxoid ◽  
In Vitro Studies ◽  
Delayed Hypersensitivity

Transplantation of Adipose-derived Cells for Periodontal Regeneration: A Systematic Review

2019 ◽  
Vol 14 (6) ◽  
pp. 504-518 ◽  
Author(s):  
Dilcele Silva Moreira Dziedzic ◽  
Bassam Felipe Mogharbel ◽  
Priscila Elias Ferreira ◽  
Ana Carolina Irioda ◽  
Katherine Athayde Teixeira de Carvalho
Keyword(s):  
Systematic Review ◽  
Animal Models ◽  
Platelet Rich Plasma ◽  
Periodontal Regeneration ◽  
In Vitro Studies ◽  
In Vivo Studies ◽  
Cell Sources ◽  
Study Designs

This systematic review evaluated the transplantation of cells derived from adipose tissue for applications in dentistry. SCOPUS, PUBMED and LILACS databases were searched for in vitro studies and pre-clinical animal model studies using the keywords “ADIPOSE”, “CELLS”, and “PERIODONTAL”, with the Boolean operator “AND”. A total of 160 titles and abstracts were identified, and 29 publications met the inclusion criteria, 14 in vitro and 15 in vivo studies. In vitro studies demonstrated that adipose- derived cells stimulate neovascularization, have osteogenic and odontogenic potential; besides adhesion, proliferation and differentiation on probable cell carriers. Preclinical studies described improvement of bone and periodontal healing with the association of adipose-derived cells and the carrier materials tested: Platelet Rich Plasma, Fibrin, Collagen and Synthetic polymer. There is evidence from the current in vitro and in vivo data indicating that adipose-derived cells may contribute to bone and periodontal regeneration. The small quantity of studies and the large variation on study designs, from animal models, cell sources and defect morphology, did not favor a meta-analysis. Additional studies need to be conducted to investigate the regeneration variability and the mechanisms of cell participation in the processes. An overview of animal models, cell sources, and scaffolds, as well as new perspectives are provided for future bone and periodontal regeneration study designs.

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