Adherence of a virulent strain of Listeria monocytogenes to the surface of a hepatocarcinoma cell line via lectin-substrate interaction

1990 ◽  
Vol 153 (3) ◽  
pp. 282-286 ◽  
Author(s):  
Richard E. Cowart ◽  
Jennifer Lashmet ◽  
Margie E. McIntosh ◽  
Todd J. Adams
Keyword(s):  
Listeria Monocytogenes ◽  
Cell Line ◽  
Virulent Strain ◽  
Substrate Interaction ◽  
Hepatocarcinoma Cell ◽  
Hepatocarcinoma Cell Line

Production of infectious ferret hepatitis E virus in a human hepatocarcinoma cell line PLC/PRF/5

2016 ◽  
Vol 213 ◽  
pp. 283-288 ◽  
Author(s):  
Tian-Cheng Li ◽  
Sayaka Yoshizaki ◽  
Tingting Yang ◽  
Michiyo Kataoka ◽  
Tomofumi Nakamura ◽  
...  
Keyword(s):  
Cell Line ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Hepatocarcinoma Cell ◽  
Human Hepatocarcinoma Cell Line ◽  
Hepatocarcinoma Cell Line

scholarly journals The antiproliferative effect of mulberry (Morus alba L.) plant on hepatocarcinoma cell line HepG2

2013 ◽  
Vol 14 (4) ◽  
pp. 375-382 ◽  
Author(s):  
Shadia A. Fathy ◽  
Abdel Nasser B. Singab ◽  
Sara A. Agwa ◽  
Dalia M. Abd El Hamid ◽  
Fatma A. Zahra ◽  
...  
Keyword(s):  
Cell Line ◽  
Morus Alba ◽  
Antiproliferative Effect ◽  
Hepatocarcinoma Cell ◽  
Hepatocarcinoma Cell Line

scholarly journals Mytoxin B and Myrothecine A Induce Apoptosis in Human Hepatocarcinoma Cell Line SMMC-7721 via PI3K/Akt Signaling Pathway

Molecules ◽  
2019 ◽  
Vol 24 (12) ◽  
pp. 2291 ◽  
Author(s):  
Huiliang Song ◽  
Yi Fu ◽  
Dan Wan ◽  
Wenjing Xia ◽  
Fengwei Lyu ◽  
...  
Keyword(s):  
Western Blot ◽  
Cell Line ◽  
Blot Analysis ◽  
Western Blot Analysis ◽  
Akt Signaling ◽  
Akt Signaling Pathway ◽  
Hepatocarcinoma Cell ◽  
Apoptotic Protein ◽  
Human Hepatocarcinoma Cell Line ◽  
Hepatocarcinoma Cell Line

Trichothecene macrolides comprise a class of valuable leading compounds in developing anticancer drugs, however, there are few reports concerning their anticancer mechanisms, especially the anticancer mechanism of the 10,13-cyclotrichothecane derivatives that are found mainly in symbiotic fungi. In vitro anticancer activity of two trichothecene macrolides mytoxin B and myrothecine A against the human hepatocarcinoma cell line SMMC-7721 was investigated in the present study. MTT assay showed that mytoxin B and myrothecine A inhibited the proliferation of SMMC-7721 cells in dose- and time-dependent manners. Annexin V-FITC/PI dual staining assay revealed that mytoxin B and myrothecine A both could induce SMMC-7721 cells apoptosis in a dose-dependent manner. The decreased expression level of anti-apoptotic protein Bcl-2 and the increased expression level of pro-apoptotic protein Bax were observed apparently in Western blot analysis. The reduced ratio of Bcl-2/Bax further confirmed the apoptosis-inducing effect of mytoxin B and myrothecine A on SMMC-7721 cells. Moreover, the expression levels of caspases-3, -8, and -9, and cleaved caspases-3, -8, and -9 were all upregulated in both mytoxin B and myrothecine A-treated cells in Western blot analysis, which indicated that both compounds might induce SMMC-7721 cells apoptosis through not only the death receptor pathway but also the mitochondrial pathway. Finally, mytoxin B and myrothecine A were found to reduce the activity of PI3K/Akt signaling pathway that was similar to the effect of LY294002 (a potent and specific PI3K inhibitor), suggesting that both mytoxin B and myrothecine A might induce SMMC-7721 cells apoptosis via PI3K/Akt pathway.

scholarly journals Chloroquine Potentiates Primaquine Activity against Active and Latent Hepatic Plasmodia Ex Vivo: Potentials and Pitfalls

2020 ◽  
Vol 65 (1) ◽  
pp. e01416-20
Author(s):  
Laurent Dembélé ◽  
Jean-François Franetich ◽  
Valérie Soulard ◽  
Nadia Amanzougaghene ◽  
Shahin Tajeri ◽  
...  
Keyword(s):  
Cell Line ◽  
Ex Vivo ◽  
Plasmodium Yoelii ◽  
Human Hepatocytes ◽  
Dependent Manner ◽  
Primary Hepatocytes ◽  
Content Type ◽  
Primary Human Hepatocytes ◽  
Hepatocarcinoma Cell ◽  
Hepatocarcinoma Cell Line

ABSTRACTFor a long while, 8-aminoquinoline compounds have been the only therapeutic agents against latent hepatic malaria parasites. These have poor activity against the blood-stage plasmodia causing acute malaria and must be used in conjunction with partner blood schizontocidal agents. We examined the impacts of one such agent, chloroquine, upon the activity of primaquine, an 8-aminoquinoline, against hepatic stages of Plasmodium cynomolgi, Plasmodium yoelii, Plasmodium berghei, and Plasmodium falciparum within several ex vivo systems—primary hepatocytes of Macaca fascicularis, primary human hepatocytes, and stably transformed human hepatocarcinoma cell line HepG2. Primaquine exposures to formed hepatic schizonts and hypnozoites of P. cynomolgi in primary simian hepatocytes exhibited similar 50% inhibitory concentration (IC50) values near 0.4 μM, whereas chloroquine in the same system exhibited no inhibitory activities. Combining chloroquine and primaquine in this system decreased the observed primaquine IC50 for all parasite forms in a chloroquine dose-dependent manner by an average of 18-fold. Chloroquine also decreased the primaquine IC50 against hepatic P. falciparum in primary human hepatocytes, P. berghei in simian primary hepatocytes, and P. yoelii in primary human hepatocytes. Chloroquine had no impact on primaquine IC50 against P. yoelii in HepG2 cells and, likewise, had no impact on the IC50 of atovaquone (hepatic schizontocide) against P. falciparum in human hepatocytes. We describe important sources of variability in the potentiation of primaquine activity by chloroquine in these systems. Chloroquine potentiated primaquine activity against hepatic forms of several plasmodia. We conclude that chloroquine specifically potentiated 8-aminoquinoline activities against active and dormant hepatic-stage plasmodia in normal primary hepatocytes but not in a hepatocarcinoma cell line.

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