Formylmethanofuran: tetrahydromethanopterin formyltransferase and N 5,N 10-methylenetetrahydromethanopterin dehydrogenase from the sulfate-reducing Archaeoglobus fulgidus: similarities with the enzymes from methanogenic Archaea

1993 ◽  
Vol 159 (3) ◽  
pp. 225-232 ◽  
Author(s):  
B. Schw�rer ◽  
J. Breitung ◽  
A. R. Klein ◽  
K. O. Stetter ◽  
R. K. Thauer
Keyword(s):  
Methanogenic Archaea ◽  
Archaeoglobus Fulgidus ◽  
Sulfate Reducing

scholarly journals Microbial Diversity Dynamics in a Methanogenic-Sulfidogenic UASB Reactor

2021 ◽  
Vol 18 (3) ◽  
pp. 1305
Author(s):  
E. Fernández-Palacios ◽  
Xudong Zhou ◽  
Mabel Mora ◽  
David Gabriel
Keyword(s):  
Paper Industry ◽  
Chemical Species ◽  
Methanogenic Archaea ◽  
Granular Sludge ◽  
Upflow Anaerobic Sludge Blanket ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Rrna Gene ◽  
Sulfate Reducing ◽  
Long Run

In this study, the long-term performance and microbial dynamics of an Upflow Anaerobic Sludge Blanket (UASB) reactor targeting sulfate reduction in a SOx emissions treatment system were assessed using crude glycerol as organic carbon source and electron donor under constant S and C loading rates. The reactor was inoculated with granular sludge obtained from a pulp and paper industry and fed at a constant inlet sulfate concentration of 250 mg S-SO42−L−1 and a constant C/S ratio of 1.5 ± 0.3 g Cg−1 S for over 500 days. Apart from the regular analysis of chemical species, Illumina analyses of the 16S rRNA gene were used to study the dynamics of the bacterial community along with the whole operation. The reactor was sampled along the operation to monitor its diversity and the changes in targeted species to gain insight into the performance of the sulfidogenic UASB. Moreover, studies on the stratification of the sludge bed were performed by sampling at different reactor heights. Shifts in the UASB performance correlated well with the main shifts in microbial communities of interest. A progressive loss of the methanogenic capacity towards a fully sulfidogenic UASB was explained by a progressive wash-out of methanogenic Archaea, which were outcompeted by sulfate-reducing bacteria. Desulfovibrio was found as the main sulfate-reducing genus in the reactor along time. A progressive reduction in the sulfidogenic capacity of the UASB was found in the long run due to the accumulation of a slime-like substance in the UASB.

scholarly journals Unusual Starch Degradation Pathway via Cyclodextrins in the Hyperthermophilic Sulfate-Reducing Archaeon Archaeoglobus fulgidus Strain 7324

2007 ◽  
Vol 189 (24) ◽  
pp. 8901-8913 ◽  
Author(s):  
Antje Labes ◽  
Peter Schönheit
Keyword(s):  
Degradation Pathway ◽  
Archaeoglobus Fulgidus ◽  
Starch Degradation ◽  
Cyclodextrin Glucanotransferase ◽  
Gene Encoding ◽  
Thermococcus Litoralis ◽  
Sulfate Reducing ◽  
Combined Action ◽  
Archaeoglobus Fulgidus Strain 7324 ◽  
Modified Embden Meyerhof Pathway

ABSTRACT The hyperthermophilic archaeon Archaeoglobus fulgidus strain 7324 has been shown to grow on starch and sulfate and thus represents the first sulfate reducer able to degrade polymeric sugars. The enzymes involved in starch degradation to glucose 6-phosphate were studied. In extracts of starch-grown cells the activities of the classical starch degradation enzymes, α-amylase and amylopullulanase, could not be detected. Instead, evidence is presented here that A. fulgidus utilizes an unusual pathway of starch degradation involving cyclodextrins as intermediates. The pathway comprises the combined action of an extracellular cyclodextrin glucanotransferase (CGTase) converting starch to cyclodextrins and the intracellular conversion of cyclodextrins to glucose 6-phosphate via cyclodextrinase (CDase), maltodextrin phosphorylase (Mal-P), and phosphoglucomutase (PGM). These enzymes, which are all induced after growth on starch, were characterized. CGTase catalyzed the conversion of starch to mainly β-cyclodextrin. The gene encoding CGTase was cloned and sequenced and showed highest similarity to a glucanotransferase from Thermococcus litoralis. After transport of the cyclodextrins into the cell by a transport system to be defined, these molecules are linearized via a CDase, catalyzing exclusively the ring opening of the cyclodextrins to the respective maltooligodextrins. These are degraded by a Mal-P to glucose 1-phosphate. Finally, PGM catalyzes the conversion of glucose 1-phosphate to glucose 6-phosphate, which is further degraded to pyruvate via the modified Embden-Meyerhof pathway.

scholarly journals Physiology, Biochemistry, and Applications of F420- and Fo-Dependent Redox Reactions

2016 ◽  
Vol 80 (2) ◽  
pp. 451-493 ◽  
Author(s):  
Chris Greening ◽  
F. Hafna Ahmed ◽  
A. Elaaf Mohamed ◽  
Brendon M. Lee ◽  
Gunjan Pandey ◽  
...  
Keyword(s):  
Redox Reactions ◽  
Methanogenic Archaea ◽  
Content Type ◽  
Sulfate Reducing ◽  
Single Function ◽  
Wide Range ◽  
Domains Of Life ◽  
Photochemical Properties ◽  
Endogenous Metabolites ◽  
Dependent Processes

SUMMARY5-Deazaflavin cofactors enhance the metabolic flexibility of microorganisms by catalyzing a wide range of challenging enzymatic redox reactions. While structurally similar to riboflavin, 5-deazaflavins have distinctive and biologically useful electrochemical and photochemical properties as a result of the substitution of N-5 of the isoalloxazine ring for a carbon. 8-Hydroxy-5-deazaflavin (Fo) appears to be used for a single function: as a light-harvesting chromophore for DNA photolyases across the three domains of life. In contrast, its oligoglutamyl derivative F420is a taxonomically restricted but functionally versatile cofactor that facilitates many low-potential two-electron redox reactions. It serves as an essential catabolic cofactor in methanogenic, sulfate-reducing, and likely methanotrophic archaea. It also transforms a wide range of exogenous substrates and endogenous metabolites in aerobic actinobacteria, for example mycobacteria and streptomycetes. In this review, we discuss the physiological roles of F420in microorganisms and the biochemistry of the various oxidoreductases that mediate these roles. Particular focus is placed on the central roles of F420in methanogenic archaea in processes such as substrate oxidation, C1pathways, respiration, and oxygen detoxification. We also describe how two F420-dependent oxidoreductase superfamilies mediate many environmentally and medically important reactions in bacteria, including biosynthesis of tetracycline and pyrrolobenzodiazepine antibiotics by streptomycetes, activation of the prodrugs pretomanid and delamanid byMycobacterium tuberculosis, and degradation of environmental contaminants such as picrate, aflatoxin, and malachite green. The biosynthesis pathways of Foand F420are also detailed. We conclude by considering opportunities to exploit deazaflavin-dependent processes in tuberculosis treatment, methane mitigation, bioremediation, and industrial biocatalysis.

Full scale fluidized bed anaerobic reactor for domestic wastewater treatment: performance, sludge production and biofilm

2004 ◽  
Vol 49 (11-12) ◽  
pp. 319-325 ◽  
Author(s):  
N.M. Mendonça ◽  
C.L. Niciura ◽  
E.P. Gianotti ◽  
J.R. Campos
Keyword(s):  
Wastewater Treatment ◽  
Fluidized Bed ◽  
Methanogenic Archaea ◽  
Domestic Wastewater ◽  
Full Scale ◽  
Anaerobic Reactor ◽  
Sulfate Reducing ◽  
Domestic Wastewater Treatment ◽  
The Difference ◽  
Sludge Production

This paper describes the performance, sludge production and biofilm characteristics of a full scale fluidized bed anaerobic reactor (32 m3) for domestic wastewater treatment. The reactor was operated with 10.5 m.h-1 upflow velocity, 3.2 h hydraulic retention time, and recirculation ratio of 0.85 and it presented removal efficiencies of 71 ± 8% of COD and 77 ± 14% of TSS. During the apparent steady-state period, specific sludge production and sludge age in the reactor were (0.116 ± 0.033) kgVSS. kgCOD-1 and (12 ± 5)d, respectively. Biofilm formed in the reactor presented two different patterns: one of them at the beginning of the colonization and the other of mature biofilm. These different colonization patterns are due to bed stratification in the reactor, caused by the difference in local-energy dissipation rates along the reactor's height, and density, shape, etc. of the bioparticles. The biofilm population is formed mainly of syntrophic consortia among sulfate reducing bacteria, methanogenic archaea such as Methanobacterium and Methanosaeta-like cells.

scholarly journals Trace Metal Imaging of Sulfate-Reducing Bacteria and Methanogenic Archaea at Single-Cell Resolution by Synchrotron X-Ray Fluorescence Imaging

2017 ◽  
Vol 35 (1) ◽  
pp. 81-89 ◽  
Author(s):  
Jennifer B. Glass ◽  
Si Chen ◽  
Katherine S. Dawson ◽  
Damian R. Horton ◽  
Stefan Vogt ◽  
...  
Keyword(s):  
Trace Metal ◽  
Single Cell ◽  
Fluorescence Imaging ◽  
Methanogenic Archaea ◽  
Sulfate Reducing Bacteria ◽  
X Ray ◽  
Sulfate Reducing ◽  
Reducing Bacteria
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