Enzymes involved in crotonate metabolism in Syntrophomonas wolfei

1992 ◽  
Vol 158 (5) ◽  
pp. 344-349 ◽  
Author(s):  
Michael J. McInerney ◽  
Neil Q. Wofford
Keyword(s):  
Syntrophomonas Wolfei

scholarly journals Draft Genome Sequence of Syntrophomonas wolfei subsp. methylbutyratica Strain 4J5 T (JCM 14075), a Mesophilic Butyrate- and 2-Methylbutyrate-Degrading Syntroph

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Takashi Narihiro ◽  
Masaru K. Nobu ◽  
Hideyuki Tamaki ◽  
Yoichi Kamagata ◽  
Wen-Tso Liu
Keyword(s):  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Syntrophomonas Wolfei ◽  
Mesophilic Bacterium

Syntrophomonas wolfei subsp. methylbutyratica strain 4J5 T (=JCM 14075 T ) is a mesophilic bacterium capable of degrading butyrate and 2-methylbutyrate through syntrophic cooperation with a partner methanogen. The draft genome sequence is 3.2 Mb, with a G+C content of 45.5%.

scholarly journals Proteomic analysis reveals metabolic and regulatory systems involved in the syntrophic and axenic lifestyle of Syntrophomonas wolfei

2015 ◽  
Vol 6 ◽  
Author(s):  
Jessica R. Sieber ◽  
Bryan R. Crable ◽  
Cody S. Sheik ◽  
Gregory B. Hurst ◽  
Lars Rohlin ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Regulatory Systems ◽  
Syntrophomonas Wolfei

scholarly journals A Proteomic View at the Biochemistry of Syntrophic Butyrate Oxidation in Syntrophomonas wolfei

PLoS ONE ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. e56905 ◽  
Author(s):  
Alexander Schmidt ◽  
Nicolai Müller ◽  
Bernhard Schink ◽  
David Schleheck
Keyword(s):  
Syntrophomonas Wolfei

scholarly journals Efficient Metabolic Exchange and Electron Transfer within a Syntrophic Trichloroethene-Degrading Coculture of Dehalococcoides mccartyi 195 and Syntrophomonas wolfei

2015 ◽  
Vol 81 (6) ◽  
pp. 2015-2024 ◽  
Author(s):  
Xinwei Mao ◽  
Benoit Stenuit ◽  
Alexandra Polasko ◽  
Lisa Alvarez-Cohen
Keyword(s):  
Pure Culture ◽  
Threshold Value ◽  
Content Type ◽  
Dehalococcoides Mccartyi ◽  
Atp Binding Cassette Transporter ◽  
Syntrophomonas Wolfei ◽  
Membrane Bound ◽  
Butyrate Fermentation ◽  
20 Nm ◽  
Metabolic Exchange

ABSTRACTDehalococcoides mccartyi195 (strain 195) andSyntrophomonas wolfeiwere grown in a sustainable syntrophic coculture using butyrate as an electron donor and carbon source and trichloroethene (TCE) as an electron acceptor. The maximum dechlorination rate (9.9 ± 0.1 μmol day−1) and cell yield [(1.1 ± 0.3) × 108cells μmol−1Cl−] of strain 195 maintained in coculture were, respectively, 2.6 and 1.6 times higher than those measured in the pure culture. The strain 195 cell concentration was about 16 times higher than that ofS. wolfeiin the coculture. Aqueous H2concentrations ranged from 24 to 180 nM during dechlorination and increased to 350 ± 20 nM when TCE was depleted, resulting in cessation of butyrate fermentation byS. wolfeiwith a theoretical Gibbs free energy of −13.7 ± 0.2 kJ mol−1. Carbon monoxide in the coculture was around 0.06 μmol per bottle, which was lower than that observed for strain 195 in isolation. The minimum H2threshold value for TCE dechlorination by strain 195 in the coculture was 0.6 ± 0.1 nM. Cell aggregates during syntrophic growth were observed by scanning electron microscopy. The interspecies distances to achieve H2fluxes required to support the measured dechlorination rates were predicted using Fick's law and demonstrated the need for aggregation. Filamentous appendages and extracellular polymeric substance (EPS)-like structures were present in the intercellular spaces. The transcriptome of strain 195 during exponential growth in the coculture indicated increased ATP-binding cassette transporter activities compared to the pure culture, while the membrane-bound energy metabolism related genes were expressed at stable levels.

Adhesion of Biodegradative Anaerobic Bacteria to Solid Surfaces

1999 ◽  
Vol 65 (11) ◽  
pp. 5082-5088 ◽  
Author(s):  
Paula M. van Schie ◽  
Madilyn Fletcher
Keyword(s):  
Anaerobic Bacteria ◽  
Solid Surfaces ◽  
The Other ◽  
Strictly Anaerobic ◽  
Anoxic Environments ◽  
Syntrophomonas Wolfei ◽  
Subsurface Environments ◽  
Desulfomonile Tiedjei ◽  
Glass Surfaces

ABSTRACT In order to exploit the ability of anaerobic bacteria to degrade certain contaminants for bioremediation of polluted subsurface environments, we need to understand the mechanisms by which such bacteria partition between aqueous and solid phases, as well as the environmental conditions that influence partitioning. We studied four strictly anaerobic bacteria, Desulfomonile tiedjei,Syntrophomonas wolfei, Syntrophobacter wolinii, and Desulfovibrio sp. strain G11, which theoretically together can constitute a tetrachloroethylene- and trichloroethylene-dechlorinating consortium. Adhesion of these organisms was evaluated by microscopic determination of the numbers of cells that attached to glass coverslips exposed to cell suspensions under anaerobic conditions. We studied the effects of the growth phase of the organisms on adhesion, as well as the influence of electrostatic and hydrophobic properties of the substratum. Results indicate thatS. wolfei adheres in considerably higher numbers to glass surfaces than the other three organisms. Starvation greatly decreases adhesion of S. wolfei and Desulfovibrio sp. strain G11 but seems to have less of an effect on the adhesion of the other bacteria. The presence of Fe3+ on the substratum, which would be electropositive, significantly increased the adhesion ofS. wolfei, whereas the presence of silicon hydrophobic groups decreased the numbers of attached cells of all species. Measurements of transport of cells through hydrophobic-interaction and electrostatic-interaction columns indicated that all four species had negatively charged cell surfaces and that D. tiedjei andDesulfovibrio sp. strain G11 possessed some hydrophobic cell surface properties. These findings are an early step toward understanding the dynamic attachment of anaerobic bacteria in anoxic environments.

Sign in / Sign up

Export Citation Format

Share Document