Restriction endonuclease digestion of meiotic chromosomes of Allium subvillosum L.

Genetica ◽  
1993 ◽  
Vol 87 (3) ◽  
Author(s):  
J. Fernandez-Piqueras ◽  
R. Lozano ◽  
C. Sentis ◽  
M. Ruiz Rej�n
Keyword(s):  
Restriction Endonuclease ◽  
Restriction Endonuclease Digestion ◽  
Meiotic Chromosomes ◽  
Endonuclease Digestion

Restriction endonuclease digestion of meiotic and mitotic chromosomes in Pyrgomorpha conica (Orthoptera: Pyrgomorphidae)

Genome ◽  
1988 ◽  
Vol 30 (5) ◽  
pp. 621-626 ◽  
Author(s):  
C. López-Fernández ◽  
J. Gosálvez ◽  
J. A. Suja ◽  
R. Mezzanotte
Keyword(s):  
Restriction Endonuclease ◽  
Chromatin Condensation ◽  
Restriction Endonucleases ◽  
Restriction Endonuclease Digestion ◽  
Mitotic Chromosomes ◽  
Meiotic Chromosomes ◽  
Restriction Sites ◽  
Endonuclease Digestion

Mitotic and meiotic chromosomes of the grasshopper Pyrgomorpha conica were treated with the restriction endonucleases (REs) AluI, MboI, and HaeIII. Two important results were obtained. First, REs successfully acted to produce linear differentiation not only in mitotic but also in meiotic chromosomes. Second, the changes in pycnosis that occur in the chromatin from first prophase to first metaphase in meiosis, as revealed with orcein or Giemsa, do not affect the digestion capability of REs. This suggests that it is the presence of restriction sites, rather than chromatin condensation itself, that is critical in the production of the linear differentiation obtained.Key words: insect cytogenetics, restriction endonucleases, mitosis, meiosis, heterochromatin.

Amplification and restriction endonuclease digestion of the Pr1 gene for the detection and characterization of Metarhizium strains

1997 ◽  
Vol 101 (3) ◽  
pp. 257-265 ◽  
Author(s):  
Soraya C.M. Leal ◽  
David J. Bertioli ◽  
Tariq M. Butt ◽  
John H. Carder ◽  
Paul R. Burrows ◽  
...  
Keyword(s):  
Restriction Endonuclease ◽  
Restriction Endonuclease Digestion ◽  
Endonuclease Digestion
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