Functional antagonism by a monoclonal antibody to digoxin in a test system of cultured rat heart myocytes

1996 ◽  
Vol 160-161 (1) ◽  
pp. 117-120 ◽  
Author(s):  
G. Wallukat ◽  
H. -U. Simon ◽  
W. -D. M�ller ◽  
I. Wolf
Keyword(s):  
Monoclonal Antibody ◽  
Rat Heart ◽  
Test System ◽  
Rat Heart Myocytes ◽  
Functional Antagonism

Spectral analysis of cytochromes in rat heart myocytes: Transient and steady-state photodiode array spectrophotometry measurements

1992 ◽  
Vol 299 (1) ◽  
pp. 8-14 ◽  
Author(s):  
Paolo Sarti ◽  
Giovanni Antonini ◽  
Francesco Malatesta ◽  
Emilio D'Itri ◽  
Maurizio Brunori ◽  
...  
Keyword(s):  
Spectral Analysis ◽  
Steady State ◽  
Rat Heart ◽  
Photodiode Array ◽  
Rat Heart Myocytes ◽  
Transient And Steady State

Intracellular free Ca2+ and the hypercontracture of adult rat heart myocytes

1986 ◽  
Vol 245 (2) ◽  
pp. 426-435 ◽  
Author(s):  
Mindy R. Lambert ◽  
J.David Johnson ◽  
Karla G. Lamka ◽  
Gerald P. Brierley ◽  
Ruth A. Altschuld
Keyword(s):  
Rat Heart ◽  
Adult Rat ◽  
Rat Heart Myocytes

Sodium-dependent recovery of ionised magnesium concentration following magnesium load in rat heart myocytes

2005 ◽  
Vol 451 (5) ◽  
pp. 657-667 ◽  
Author(s):  
Hasan A. Almulla ◽  
Peter G. Bush ◽  
Michael G. Steele ◽  
Peter W. Flatman ◽  
David Ellis
Keyword(s):  
Rat Heart ◽  
Magnesium Concentration ◽  
Sodium Dependent ◽  
Rat Heart Myocytes

scholarly journals Release of enzymes from adult rat heart myocytes.

1982 ◽  
Vol 51 (5) ◽  
pp. 560-568 ◽  
Author(s):  
M P Murphy ◽  
C Hohl ◽  
G P Brierley ◽  
R A Altschuld
Keyword(s):  
Rat Heart ◽  
Adult Rat ◽  
Rat Heart Myocytes

Changes in expression of a functional Gi protein in cultured rat heart cells

1988 ◽  
Vol 255 (1) ◽  
pp. C51-C59 ◽  
Author(s):  
I. S. Allen ◽  
S. T. Gaa ◽  
T. B. Rogers
Keyword(s):  
Adenylate Cyclase ◽  
Pertussis Toxin ◽  
Rat Heart ◽  
Neonatal Rat ◽  
Heart Cells ◽  
Dependent Manner ◽  
Camp Accumulation ◽  
Ang Ii ◽  
Rat Heart Myocytes ◽  
Neonatal Rat Heart

The muscarinic cholinergic agonist, carbachol, and pertussis toxin were used to examine the functional status of the guanine nucleotide-binding protein that inhibits adenylate cyclase (Gi) in cultured neonatal rat heart myocytes. The isoproterenol stimulation of adenylate cyclase activity in myocyte membranes and adenosine 3',5'-cyclic monophosphate (cAMP) accumulation in intact cells (4 days in culture) were insensitive to carbachol (0.1 mM). However, in cells cultured for 11 days, carbachol (0.1 mM) inhibited isoproterenol-stimulated cAMP accumulation by 30%. Angiotensin II (ANG II) was also found to inhibit isoproterenol-stimulated cAMP accumulation in day 11 cells in a dose-dependent manner. Pertussis toxin treatment reversed the inhibitory effects of both ANG II and carbachol, suggesting a role for Gi in the process. Carbachol binding to membranes from day 4 cells was relatively insensitive to guanine nucleotides when compared with binding to membranes from day 11 or adult cells. Furthermore, pertussis toxin-mediated 32P incorporation into a 39- to 41-kDa substrate in day 11 membranes was increased 3.2-fold over that measured in day 4 membranes. These findings support the view that, although Gi is expressed, it is nonfunctional in 4-day-old cultured neonatal rat heart myocytes and acquisition of functional Gi is dependent on culture conditions. Furthermore, the ANG II receptor can couple to Gi in heart.

Permeability of rat heart myocytes to cytochrome c

1999 ◽  
Vol 56 (11-12) ◽  
pp. 1061-1069 ◽  
Author(s):  
P. Sarti ◽  
R. B. Silver ◽  
L. Paroli ◽  
I. Nikonorov ◽  
T. J. J. Blanck
Keyword(s):  
Cytochrome C ◽  
Rat Heart ◽  
Rat Heart Myocytes

Cholesterol distribution in rat heart myocytes

1995 ◽  
Vol 268 (2) ◽  
pp. H759-H766
Author(s):  
H. Shmeeda ◽  
D. Petkova ◽  
Y. Barenholz
Keyword(s):  
Plasma Membrane ◽  
Rat Heart ◽  
Cholesterol Oxidase ◽  
Neonatal Rat ◽  
Relative Distribution ◽  
Intact Cells ◽  
Cholesterol Levels ◽  
Rat Heart Myocytes ◽  
Small Unilamellar Vesicles ◽  
Cholesterol Monohydrate

Cholesterol oxidase was used to investigate the distribution of free cholesterol between plasma membrane and intracellular pools in cultured neonatal rat heart myocytes. Only 20% of the total unesterified cholesterol was converted to delta 4-cholestenone by cholesterol oxidase in intact cells. With increasing age in culture and concurrent hypertrophy, there was an increase in unesterified cellular cholesterol and plasma membrane cholesterol; their relative distribution remained unchanged. Electron micrographs of negatively stained samples of day 4 cytosol revealed the presence of vesicles 50–200 nm in diameter. Cholesterol monohydrate crystals were found in the cytosol of hypertrophic day 14 cells. Treatment of day 14 cells with small unilamellar vesicles of egg phosphatidylcholine reduced plasma membrane and intracellular cholesterol levels, resulting in the disappearance of the cholesterol monohydrate crystals and the formation of vesicles smaller than those observed in day 4 cultures.

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