Mass propagation of shoots of Stevia rebaudiana using a large scale bioreactor

1994 ◽  
Vol 13-13 (3-4) ◽  
Author(s):  
Motomu Akita ◽  
Takeo Shigeoka ◽  
Yoko Koizumi ◽  
Michio Kawamura
Keyword(s):  
Large Scale ◽  
Stevia Rebaudiana ◽  
Mass Propagation ◽  
Large Scale Bioreactor

scholarly journals Salinity Effects on Gene Expression, Morphological, and Physio-Biochemical Responses of Stevia rebaudiana Bertoni In Vitro

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 820
Author(s):  
Clara Azzam ◽  
Sudad Al-Taweel ◽  
Ranya Abdel-Aziz ◽  
Karim Rabea ◽  
Alaa Abou-Sreea ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Biochemical Parameters ◽  
Large Scale ◽  
Stevia Rebaudiana ◽  
Multiple Shoots ◽  
High Salt ◽  
Stress Factors ◽  
Stevia Rebaudiana Bertoni ◽  
Nacl Concentration

Stevia rebaudiana Bertoni is a little bush, which is cultivated on a large scale in many countries for medicinal purposes and used as a natural sweetener in food products. The present work aims to conduct a protocol for stevia propagation in vitro to produce and introduce Stevia rebaudiana plants as a new sweetener crop to Egyptian agriculture. To efficiently maximize its propagation, it is important to study the influence of stress factors on the growth and development of Stevia rebaudiana grown in vitro. Two stevia varieties were investigated (Sugar High A3 and Spanti) against salt stress. Leaves were used as the source of explants for callus initiation, regeneration, multiplication and rooting. Some stress-related traits, i.e., photosynthetic pigments, proline contents, and enzyme activity for peroxidase (POD), polyphenol oxidase (PPO), and malate dehydrogenase (MDH) were studied. Murashig and Skoog (MS) medium was supplemented with four NaCl concentrations: 500, 1000, 2000, and 3000 mgL−1, while a salt-free medium was used as the control. The data revealed that salinity negatively affected all studied characters: the number of surviving calli, regeneration%, shoot length, the number of multiple shoots, number of leaf plantlets−1, number of root plantlets−1, and root length. The data also revealed that Sugar High A3 is more tolerant than Spanti. The total chlorophyll content decreased gradually with increasing NaCl concentration. However, the opposite was true for proline content. Isozyme’s fractionation exhibited high levels of variability among the two varieties. Various biochemical parameters associated with salt tolerance were detected in POD. Namely, POD4, POD6, POD 9 at an Rf of 0.34, 0.57, and 0.91 in the Sugar High A3 variety under high salt concentration conditions, as well as POD 10 at an Rf of 0.98 in both varieties under high salt concentrations. In addition, the overexpression of POD 5 and POD 10 at Rf 0.52 and 0.83 was found in both varieties at high NaCl concentrations. Biochemical parameters associated with salt tolerance were detected in PPO (PPO1, PPO2 and PPO4 at an Rf of 0.38, 0.42 and 0.62 in the Sugar High A3 variety under high salt concentrations) and MDH (MDH 3 at an Rf of 0.40 in both varieties at high salt concentrations). Therefore, these could be considered as important biochemical markers associated with salt tolerance and could be applied in stevia breeding programs (marker-assisted selection). This investigation recommends stevia variety Sugar High A3 to be cultivated under salt conditions.

scholarly journals In vitro Mass Propagation of Artemisia (Artemisia annua L.) cv: Anamed

2014 ◽  
Vol 23 (2) ◽  
pp. 165-176 ◽  
Author(s):  
Tilahun Hailu ◽  
Balcha Abera ◽  
Gabra Mariam
Keyword(s):  
Large Scale ◽  
Artemisia Annua ◽  
Shoot Tip ◽  
Nodal Explants ◽  
Mass Propagation ◽  
Mean Values ◽  
Coffee Husk ◽  
Soil Mix ◽  
Shoot Tip Explants

An efficient in vitro propagation protocol was developed for anamed (A-3) cultivar of Artemisia annua. Two and 1.5% concentration of NaOCl treatment for 10 and 20 min were found to be optimum for sterilization of shoot tip and nodal explants, respectively. Maximum percentage (98.75 ± 2.50) shoot induction was observed from nodal explants cultured on MS supplemented with 0.8 mg/l BAP + 0.1 mg/l IBA  followed by 82.50 ± 2.88% from shoot tip explants on the same medium with 0.8 mg/l TDZ for shoot tip explants. The highest number of shoots (8.05 ± 0.66/explant) was regenerated on MS + 1 mg/l BAP + 0.1 mg/l IBA. Best rooting with mean values of 18.25 ± 0.95/explant root number and root length (6.35 ± 0.10 cm) was recorded on 1/2 MS + 0.5 mg/l IBA. Up on acclimation and transplanting, 80% survival efficiency was observed on the soil mix ratio of  2 : 1 : 1 (decomposed coffee husk, forest soil and sand, respectively). The developed regeneration protocol enables a large scale commercial production and a possible system towards the genetic improvement of this crop. D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17518 Plant Tissue Cult. & Biotech. 23(2): 165-176, 2013  (December)

Large-Scale Bioreactor Pilots for Monitoring the Long-Term Hydromechanics of MSW

2013 ◽  
Vol 17 (4) ◽  
pp. 285-294 ◽  
Author(s):  
Matthias J. Staub ◽  
Jean-Pierre Gourc ◽  
Nicolas Drut ◽  
Guillaume Stoltz ◽  
Alicia A. Mansour
Keyword(s):  
Large Scale ◽  
Large Scale Bioreactor

Large-scale bioreactor production of the herbicide-degrading Aminobacter sp. strain MSH1

2013 ◽  
Vol 98 (5) ◽  
pp. 2335-2344 ◽  
Author(s):  
Nadja Schultz-Jensen ◽  
Berith E. Knudsen ◽  
Zuzana Frkova ◽  
Jens Aamand ◽  
Tina Johansen ◽  
...  
Keyword(s):  
Large Scale ◽  
Large Scale Bioreactor

scholarly journals A Novel Method for Efficient Micropropagation of Radermachera xylocarpa (Roxb.) K. Schum., a Rare Medicinally Important Forest Species

2013 ◽  
Vol 23 (1) ◽  
Author(s):  
Manjary Sathe ◽  
Megha Vibhute ◽  
Monica Jain ◽  
Pankaj Srivastav
Keyword(s):  
Tree Species ◽  
Large Scale ◽  
In Vitro Regeneration ◽  
Forest Tree ◽  
Root Induction ◽  
Mass Propagation ◽  
Forest Tree Species ◽  
Bud Induction ◽  
Indigenous Forest

Radermachera xylocarpa (Roxb.) K. Schum. is a rare indigenous forest tree species which is utilized for its wood and medicinal properties. Due to its overexploitation and specific habitat requirements the species is restricted to limited areas. In vitro mass propagation of tree species faces various challenges and no such efforts have yet been taken in propagation of this useful plant using these methods.  In order to overcome the hurdles and understanding an urgent need of its conservation and mass propagation present authors attempt to develop a simple effective tissue culture protocol for regeneration of R. xylocarpa. Nodal explants were cultured on MS supplemented with various concentrations of cytokinins and auxins.  Among different cytokinins, maximum bud induction and proliferation was obtained in media supplemented with Kn along with IBA and for effective root induction which is tough to obtain in tree species, 100% rooting was achieved in cultures with increasing concentrations of IBA. Field survival is a major challenge with regenerated plants of forest tree species. We report here for the first time 100% survival of plants in soil by carefully standardizing the period of hardening and acclimatization procedures. A novel and effective in vitro regeneration protocol of R. xylocarpa has been successfully standardized which can be adopted for large scale propagation, reforestation and conservation of rare Radermachera xylocarpa of medicinal importance.Plant Tissue Cult. & Biotech. 23(1): 21?29, 2013 (June)DOI: http://dx.doi.org/10.3329/ptcb.v23i1.15556

scholarly journals Identification of Stevioside Using Tissue Culture-Derived Stevia (Stevia rebaudiana) Leaves

2015 ◽  
Vol 8s2 ◽  
pp. BCI.S30378 ◽  
Author(s):  
Ziaul Karim ◽  
Daisuke Uesugi ◽  
Noriyuki Nakayama ◽  
M. Monzur Hossain ◽  
Kohji Ishihara ◽  
...  
Keyword(s):  
Tissue Culture ◽  
In Vitro Culture ◽  
Large Scale ◽  
Stevia Rebaudiana ◽  
Potential Method ◽  
Modern Technology ◽  
Commercial Production ◽  
Sugar Levels ◽  
Disease Free

Stevioside is a natural sweetener from Stevia leaf, which is 300 times sweeter than sugar. It helps to reduce blood sugar levels dramatically and thus can be of benefit to diabetic people. Tissue culture is a very potential modern technology that can be used in large-scale disease-free stevia production throughout the year. We successfully produced stevia plant through in vitro culture for identification of stevioside in this experiment. The present study describes a potential method for identification of stevioside from tissue culture-derived stevia leaf. Stevioside in the sample was identified using HPLC by measuring the retention time. The percentage of stevioside content in the leaf samples was found to be 9.6%. This identification method can be used for commercial production and industrialization of stevia through in vitro culture across the world.

scholarly journals Techno-Economic Analysis of Constant-Flow Woodchip Bioreactors

2021 ◽  
Vol 64 (5) ◽  
pp. 1545-1554
Author(s):  
Lindsey M. Hartfiel ◽  
Michelle L. Soupir ◽  
Kurt A. Rosentrater
Keyword(s):  
Economic Analysis ◽  
Large Scale ◽  
Removal Rate ◽  
Unit Cost ◽  
Cost Effective ◽  
Pilot Scale ◽  
Bypass Flow ◽  
Mass Removal ◽  
Low Probability ◽  
Large Scale Bioreactor

HighlightsTechno-economic analysis was performed for multiple scales of bioreactors operated under a variety of conditions.The unit cost decreased as the bioreactor size increased.The unit cost increased in bioreactors with longer HRTs and bypass flow due to reduced treatment capacity.One large bioreactor was more cost-effective than multiple smaller bioreactors.Abstract. Woodchip denitrification bioreactors are a relatively new, edge-of-field technology used to reduce nitrate-nitrogen (NO3-N) from subsurface tile drainage. The removal rate of nitrate is influenced by many factors, including temperature, dissolved oxygen, and hydraulic residence time (HRT). The objective of this study was to conduct a techno-economic analysis (TEA) for four scales of woodchip denitrification bioreactors operating at three HRTs (2, 8, and 16 h), designed with bypass flow or with a low probability of bypass flow, to determine the cost to remove 1 kg of NO3-N at each bioreactor scale and at each HRT. Several assumptions were made: the flow rate required to achieve a 2 h HRT on a per m3 basis could be achieved at all scales, the same mass removal of NO3-N was achieved on a per cubic meter basis, and the 2 h HRT did not have any bypass flow at each scale. With these assumptions, the lowest unit cost was observed for the large-scale bioreactor sized to have a low probability of bypass flow at 16 h HRT, with a resulting cost of $0.74 kg-1 NO3-N removed. The highest unit cost was observed for the pilot-scale bioreactor designed with bypass flow to achieve a 16 h HRT at a cost of $60.13 kg-1 NO3-N removed. At longer HRTs with bypass flow, a greater percent removal of nitrate has been observed with a lower mass removal rate. By having a low probability of bypass flow in the design, a higher mass removal and percent removal of nitrate were observed, leading to the above results. Contrasting this trend, the total and annual costs were highest for the large-scale bioreactor and lowest for the pilot-scale bioreactor. However, it was determined that 783%, 280%, and 54% increases in total cost for the pilot-, small-, and medium-scale bioreactors would be incurred to implement the number of bioreactors (66, 24, and 4, respectively) required to treat the same volume of flow as one large bioreactor. These results can be used to inform future design decisions and inform stakeholders of the approximate unit cost of installing a denitrifying woodchip bioreactor over a range of expected field conditions. While a larger bioreactor with a low probability of bypass flow may represent a more cost-effective investment, the potential for unintended, negative byproducts needs to be considered in the design. Keywords: Denitrification, Nitrate, Tile drainage, Water quality, Woodchip bioreactor.

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