Effects of serotonin on cerebellar Purkinje cells are dependent on the baseline firing rate

1984 ◽  
Vol 56 (1) ◽  
Author(s):  
J.C. Strahlendorf ◽  
M. Lee ◽  
H.K. Strahlendorf
Keyword(s):  
Firing Rate ◽  
Purkinje Cells ◽  
Cerebellar Purkinje Cells

Mechanisms of Etomidate-Mediated Decrease of Spontaneous Spike Activity of Mouse Cerebellar Purkinje Cells in vivo

Pharmacology ◽  
2018 ◽  
Vol 103 (1-2) ◽  
pp. 82-92 ◽  
Author(s):  
Wen Pan ◽  
Wen-Yuan Wu ◽  
Yan-Hua Bing ◽  
Chun-Ping Chu ◽  
Ri Jin ◽  
...  
Keyword(s):  
Firing Rate ◽  
Receptor Antagonist ◽  
Purkinje Cells ◽  
Glycine Receptor ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Gabaa Receptor Antagonist ◽  
Significant Depression ◽  
Cerebellar Purkinje Cells

Etomidate is an imidazole, nonbarbiturate hypnotic agent that is increasingly used in procedural sedation. However, the effects of etomidate on the spontaneous activity of cerebellar Purkinje cells (PCs) in living mouse have not been fully understood. In this study, we investigated the effects of etomidate on the spontaneous simple spike (SS) activity of PCs in urethane-anesthetized mice by cell-attached recording and pharmacological methods. Cerebellar surface application of etomidate (50 μmol\L) reduced the SS firing rate in a concentration-dependent manner (IC50: 43.4 µmol\L). Application of either a γ-aminobutyric acid type A (GABAA) receptor antagonist, SR95531 (20 μmol\L) or a glycine receptor antagonist strychnine (10 μmol\L) significantly attenuated but not abolished the etomidate-induced decrease in PC SS firing rate. However, co-application of SR95531 (20 μmol\L) and strychnine (10 μmol\L) abolished the etomidate-induced decrease in PC SS firing rate. Moreover, intraperitoneal injection of etomidate (3 mg/kg body weight) also induced a significant depression in PC SS firing rate, which was blocked by the co-application of SR95531 and strychnine on the cerebellar surface. These results indicate that both GABAA and glycine receptors are involved in the etomidate-induced decrease in PC SS firing rate in vivo in mice.

Effect of simple spike firing mode on complex spike firing rate and waveform in cerebellar Purkinje cells in non-anesthetized mice

2004 ◽  
Vol 367 (2) ◽  
pp. 171-176 ◽  
Author(s):  
Laurent Servais ◽  
Bertrand Bearzatto ◽  
Raphaël Hourez ◽  
Bernard Dan ◽  
Serge N. Schiffmann ◽  
...  
Keyword(s):  
Firing Rate ◽  
Purkinje Cells ◽  
Complex Spike ◽  
Simple Spike ◽  
Firing Mode ◽  
Cerebellar Purkinje Cells ◽  
Spike Firing

Ultrastorcture of Cerebellar Purkinje Cells in Rats Subjected To Partial Global Cerebral Ischemia

1985 ◽  
Vol 43 ◽  
pp. 674-675
Author(s):  
R.V.W. Dimlich ◽  
M.H. Biros
Keyword(s):  
Cerebral Ischemia ◽  
Purkinje Cells ◽  
Cell Types ◽  
Microscopic Level ◽  
Global Cerebral Ischemia ◽  
Light Microscopic Level ◽  
Cerebellar Damage ◽  
Cerebellar Injury ◽  
Cerebellar Purkinje Cells ◽  
Cell Changes

In severe cerebral ischemia, Purkinje cells of the cerebellum are one of the cell types most vulnerable to anoxic damage. In the partial (forebrain) global ischemic (PGI) model of the rat, Paljärvi noted at the light microscopic level that cerebellar damage is inconsistant and when present, milder than in the telencephalon, diencephalon and rostral brain stem. Cerebellar injury was observed in 3 of 4 PGI rats following 5 minutes of reperfusion but in none of the rats after 90 min of reperfusion. To evaluate a time between these two extremes (5 and 90 min), the present investigation used the PGI model to study the effects of ischemia on the ultrastructure of cerebellar Purkinje cells in rats that were sacrificed after 30 min of reperfusion. This time also was chosen because lactic acid that is thought to contribute to ischemic cell changes in PGI is at a maximum after 30 min of reperfusion.

scholarly journals Characterization of starvation-induced autophagy in cerebellar Purkinje cells of pHluorin-mKate2-human LC3B transgenic mice

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Juan Alejandro Oliva Trejo ◽  
Isei Tanida ◽  
Chigure Suzuki ◽  
Soichiro Kakuta ◽  
Norihiro Tada ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Purkinje Cells ◽  
Cerebellar Purkinje Cells
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