Autoradiographic localization of binding sites for 3H-serotonin and 3H-ketanserin on neurones and astrocytes of cultured rat brain stem and spinal cord

1987 ◽  
Vol 65 (2) ◽  
Author(s):  
E. H�sli ◽  
L. H�sli
Keyword(s):  
Spinal Cord ◽  
Brain Stem ◽  
Rat Brain ◽  
Binding Sites

A re-examination of the Na+-independent binding of [3H]?-alanine to rat brain stem-spinal cord

1988 ◽  
Vol 13 (12) ◽  
pp. 1133-1138 ◽  
Author(s):  
L. M. Orensanz ◽  
E. Ambrosio ◽  
I. Fern�ndez ◽  
M. T. Montero
Keyword(s):  
Spinal Cord ◽  
Brain Stem ◽  
Rat Brain

Micturition reflexes in the in vitro neonatal rat brain stem-spinal cord-bladder preparation

1994 ◽  
Vol 266 (3) ◽  
pp. R658-R667 ◽  
Author(s):  
K. Sugaya ◽  
W. C. De Groat
Keyword(s):  
Spinal Cord ◽  
Electrical Stimulation ◽  
Brain Stem ◽  
Rat Brain ◽  
Bladder Wall ◽  
Spinal Reflex ◽  
Neonatal Rat ◽  
Evoked Contractions ◽  
Stimulation Of

An in vitro neonatal (1-7 day) rat brain stem-spinal cord-bladder (BSB) preparation was used to examine the central control of micturition. Isovolumetric bladder contractions occurred spontaneously or were induced by electrical stimulation of the ventrolateral brain stem, spinal cord, bladder wall (ES-BW), or by perineal tactile stimulation (PS). Transection of the spinal cord at the L1 segment increased the amplitude of ES-BW- and PS-evoked contractions, and subsequent removal of the spinal cord further increased spontaneous and ES-BW-evoked contractions but abolished PS-evoked contractions. Hexamethonium (1 mM), a ganglionic blocking agent, mimicked the effect of cord extirpation. Tetrodotoxin (1 microM) blocked ES-BW- and PS-evoked contractions but enhanced spontaneous contractions. Bicuculline methiodide (10-50 microM), a gamma-aminobutyric acid A receptor antagonist, increased the amplitude of spontaneous, ES-BW- and PS-evoked contractions. These results indicate that PS-evoked contractions are mediated by spinal reflex pathways, whereas spontaneous and ES-BW-evoked contractions that are elicited by peripheral mechanisms are subject to a tonic inhibition dependent on an efferent outflow from the spinal cord. PS-evoked micturition is also subject to inhibitory modulation arising from sites rostral to the lumbosacral spinal cord. Although electrical stimulation of bulbospinal excitatory pathways can initiate bladder contractions in the neonatal rat, these pathways do not appear to have an important role in controlling micturition during the first postnatal week.

Comparison of mu, delta, and kappa opiate binding sites in rat brain and spinal cord

Life Sciences ◽  
1984 ◽  
Vol 34 (3) ◽  
pp. 281-285 ◽  
Author(s):  
Kenneth J. Mack ◽  
A. Killian ◽  
James A. Weyhenmeyer
Keyword(s):  
Spinal Cord ◽  
Rat Brain ◽  
Binding Sites ◽  
Opiate Binding ◽  
Brain And Spinal Cord

scholarly journals Non-radioactive localization of substance P binding sites in rat brain and spinal cord using peptides labeled with 1.4-nm gold particles.

1995 ◽  
Vol 43 (8) ◽  
pp. 821-827 ◽  
Author(s):  
G Segond von Banchet ◽  
B Heppelmann
Keyword(s):  
Spinal Cord ◽  
Substance P ◽  
Rat Brain ◽  
Gold Particle ◽  
Binding Sites ◽  
New Method ◽  
Neurokinin A ◽  
Histological Sections ◽  
Native Peptide ◽  
Brain And Spinal Cord

We present here a new method for non-radioactive labeling of substance P (SP) to demonstrate the distribution of its binding sites in histological sections. The peptide was labeled at the primary amino group with a 1.4-nm gold particle. In Western blots of membrane fractions of rat spinal cord, specific binding occurred at 38 and 58 KD. This binding was competitively suppressed by adding the native peptide. In addition, the SP-gold conjugate was able to displace the corresponding 125I-labeled peptide from binding proteins. In histological sections, binding sites could be shown in various parts of rat brain and spinal cord. The distribution patterns were comparable to those found in studies using autoradiographic methods. Adding the native peptide or a neurokinin 1 receptor agonist markedly reduced labeling of the tissue, whereas only a slight reduction was obtained after adding neurokinin A. Therefore, SP could be specifically labeled with a 1.4-nm gold particle to demonstrate its binding sites. This new method combines the advantages of receptor-ligand affinity binding with a non-radioactive detection system. It can be used for labeling peptides in general and therefore offers an alternative or addition to other methods used in study of the distribution of membrane receptors.

Autoradiographic localization of [3H]thiocolchicoside binding sites in the rat brain and spinal cord

2001 ◽  
Vol 40 (8) ◽  
pp. 1044-1049 ◽  
Author(s):  
Walter Balduini ◽  
Valerio De Angelis ◽  
Erika Mazzoni ◽  
Henri Depoortere ◽  
Flaminio Cattabeni ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Rat Brain ◽  
Binding Sites ◽  
Brain And Spinal Cord
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