Somatic embryogenesis and plant regeneration from leaf-derived cell suspension of a mature tree ? Thevetia peruviana L.

1994 ◽  
Vol 14-14 (2-3) ◽  
Author(s):  
Abha Sharma ◽  
Anjani Kumar
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Cell Suspension ◽  
Mature Tree ◽  
Thevetia Peruviana

scholarly journals Cytomorphological studies on somatic embryogenesis of Gentiana tibetica (King) and G. cruciata (L.)

2014 ◽  
Vol 65 (1-2) ◽  
pp. 47-51 ◽  
Author(s):  
Anna Mikuła ◽  
Maria Wesołowska ◽  
Józef Kapusta ◽  
Lutosława Skrzypczak ◽  
Jan J. Rybczyński
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Cell Suspension ◽  
Agar Medium ◽  
Induction Medium ◽  
Mineral Salts ◽  
Fast Green ◽  
Seedling Explants ◽  
Callus Initiation ◽  
Pas Reaction

The process of plant regeneration via somatic embryogenesis of two gentianas, <em>Gentiana tibetica</em> and <em>G. cruciata</em> was described. For this purpose seedling explants were cultured on agar medium and later maintained in cell suspension. For callus initiation seedling explants like: cotyledons, hypocotyl and root were plated on a callus induction medium (CIM) composed of MS (1962), supplemented with 0.5 mg/l 2,4-D and 1.0 mg/l Kin. For the formation of cell suspension culture, embryogenic callus was transferred into liquid maintained medium (MM) composed of MS (1962), supplemented with 1.0 mg/l Dic + 0.1 mg/l NAA + 2.0 mg/l BAP + 80.0 mg/l SA. The conversion of somatic embryo into plantlets required a new medium (ECM) based on MS (1962) mineral salts, supplemented with 0.5 mg/l GA<sub>3</sub> + 1.0 mg/l Kin + 0.5 mg/l NAA. For cytomorphological studies of particular stages of embryogenesis, specimens were stained with dyes and reagents: 1. PAS reaction with leukofucsin, 2. Safranin + fast green, 3. Erlich's hematoxylin.

scholarly journals Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis

2003 ◽  
Vol 60 (4) ◽  
pp. 683-689 ◽  
Author(s):  
Mariza Monteiro ◽  
Beatriz Appezzato-da-Glória ◽  
Maria José Valarini ◽  
Carlos Alberto de Oliveira ◽  
Maria Lucia Carneiro Vieira
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Genetic Transformation ◽  
Medicago Sativa ◽  
Cell Suspension ◽  
High Efficiency ◽  
Point Of View ◽  
Ability To Regenerate

Alfalfa is one of the most frequently studied species from the production of tissue culture-derived embryos point of view. In this study, five alfalfa cultivars were analyzed with reference to their ability to regenerate plants from protoplast cultures via somatic embryogenesis. Plant regeneration from leaf-derived protoplasts isolated from the cultivar Rangelander was achieved using a protocol defined for alfalfa cell suspension-derived embryogenesis. Because of its high efficiency, this procedure is recommended for protoplast electroporation-mediated genetic transformation of alfalfa.

Sign in / Sign up

Export Citation Format

Share Document