Intergeneric somatic hybridization of sexually incompatible parents: Citrus sinensis and Atalantia ceylanica

1993 ◽  
Vol 12 (12) ◽  
pp. 687-690 ◽  
Author(s):  
E. S. Louzada ◽  
J. W. Grosser ◽  
F. G. Gmitter
2004 ◽  
Vol 39 (7) ◽  
pp. 721-724 ◽  
Author(s):  
Marcia Cristina Calixto ◽  
Francisco de Assis Alves Mourão Filho ◽  
Beatriz Madalena Januzzi Mendes ◽  
Maria Lúcia Carneiro Vieira

This work had as objective to produce citrus somatic hybrids between sweet oranges and pummelos. After chemical fusion of sweet orange embryogenic protoplasts with pummelo mesophyll-derived protoplasts, plants were regenerated by somatic embryogenesis and acclimatized in a greenhouse. The hybrids of 'Hamlin' sweet orange + 'Indian Red' pummelo and 'Hamlin' sweet orange + 'Singapura' pummelo were confirmed by leaf morphology, chromosome counting and molecular analysis. These hybrids have potential to be used directly as rootstocks aiming blight, citrus tristeza virus, and Phytophthora-induced disease tolerance, as well as for rootstocks improvement programs.


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