Regeneration of fertile transgenic indica (group 1) rice plants following microprojectile transformation of embryogenic suspension culture cells

1996 ◽  
Vol 15 (7) ◽  
pp. 465-469 ◽  
Author(s):  
Shiping Zhang ◽  
Lili Chen ◽  
Rongda Qu ◽  
Philippe Marmey ◽  
Roger Beachy ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Rice Plants ◽  
Culture Cells ◽  
Embryogenic Suspension ◽  
Embryogenic Suspension Culture ◽  
Group 1

Regeneration of fertile transgenic indica (group 1) rice plants following microprojectile transformation of embryogenic suspension culture cells

1996 ◽  
Vol 15 (7) ◽  
pp. 465-469 ◽  
Author(s):  
Lili Cheng ◽  
Rongda Qu ◽  
Philippe Marmey ◽  
Roger Beachy ◽  
Claude Fauquet ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Rice Plants ◽  
Culture Cells ◽  
Embryogenic Suspension ◽  
Embryogenic Suspension Culture ◽  
Group 1

scholarly journals Establishment of Parameters for Agrobacterium Transformation of Asparagus Embryogenic Suspension Culture Cells

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 878B-878
Author(s):  
Roger A. May ◽  
Kenneth C. Sink
Keyword(s):  
Suspension Culture ◽  
Transient Expression ◽  
Gus Expression ◽  
Gus Gene ◽  
Suspension Cells ◽  
Embryogenic Cells ◽  
Culture Cells ◽  
Embryogenic Suspension ◽  
Embryogenic Suspension Culture ◽  
Embryogenic Suspension Cultures

Conditions for Agrobacterium transformation of asparagus embryogenic suspension cells were investigated using an intron-containing GUS gene in pCNL56 to detect transformation events. Embryogenic suspension cultures of Rutgers 22 were maintained on LS medium with 5 mg NAA/liter, subcultured weekly, and used 5 days thereafter. For initial experiments, cells were inoculated at 5 x 108 cfu/ml for 15 min and cocultivated for 4 days on LS medium with 10 g Phytagel/liter (EM1 medium). Subsequently, the cells were transferred to EM1 with 200 mg Timentin/liter and tested for GUS expression after a total of 6 days. The effect of acetosyringone (AS) on four A. tumefaciens strains was tested. With or without AS induction, EHA105 and C58C1(pMP90) produced a significantly greater number of GUS foci on embryogenic cells than C58C1(pGV2260) and LBA4404. Transient expression was very low from cells inoculated with C58C1(pGV2260) and nonexistent from LBA4404. AS induced significantly more GUS foci from EHA105 and C58C1(pMP90) than from the same noninduced bacteria; however, it had no effect on C58C1(pGV2260). Upon repeating the experiment using EHA105 and C58C1(pMP90) only, no differences in response were observed, although AS induced more GUS foci from both strains and EHA105 outperformed C58C1. Inoculum density was investigated using induced EHA105. 5 x 107 cfu/ml significantly increased the number of GUS foci than 108, 5 x 108, or 109, although it was not statistically different from 107, which produced slightly fewer foci.

Cryopreservation of an embryogenic suspension culture of Picea sitchensis and subsequent plant regeneration

1993 ◽  
Vol 8 (1-4) ◽  
pp. 156-162 ◽  
Author(s):  
Jens I. Find ◽  
Franz Floto ◽  
Peter Krogstrup ◽  
Jette Dahl M⊘ller ◽  
Jens V. N⊘rgaard ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Suspension Culture ◽  
Picea Sitchensis ◽  
Embryogenic Suspension ◽  
Embryogenic Suspension Culture

Fertile wheat (Triticum aestivum L.) regenerants from protoplasts of embryogenic suspension culture

1994 ◽  
Vol 38 (1) ◽  
pp. 1-10 ◽  
Author(s):  
J�nos Pauk ◽  
Zolt�n Kert�sz ◽  
Barnab�s Jenes ◽  
L�szl� Purnhauser ◽  
Outi Manninen ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Suspension Culture ◽  
Triticum Aestivum L ◽  
Embryogenic Suspension ◽  
Embryogenic Suspension Culture

Development of an embryogenic suspension culture of bitter melon (Momordica charantia L.)

2006 ◽  
Vol 109 (2) ◽  
pp. 123-129 ◽  
Author(s):  
M. Thiruvengadam ◽  
S. Varisai Mohamed ◽  
C.H. Yang ◽  
N. Jayabalan
Keyword(s):  
Suspension Culture ◽  
Momordica Charantia ◽  
Bitter Melon ◽  
Embryogenic Suspension ◽  
Embryogenic Suspension Culture

Development of friable embryogenic callus and embryogenic suspension culture systems in cassava (Manihot esculenta Crantz)

1996 ◽  
Vol 14 (6) ◽  
pp. 726-730 ◽  
Author(s):  
Nigel J. Taylor ◽  
Michelle Edwards ◽  
Rebecca J. Kiernan ◽  
Christopher D.M. Davey ◽  
David Blakesley ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Embryogenic Callus ◽  
Manihot Esculenta ◽  
Manihot Esculenta Crantz ◽  
Culture Systems ◽  
Friable Embryogenic Callus ◽  
Embryogenic Suspension ◽  
Embryogenic Suspension Culture
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