Efficient plant regeneration from hypocotyl protoplasts of broccoli (Brassica oleracea L. ssp. italica Plenck)

1990 ◽  
Vol 9 (6) ◽  
Author(s):  
H.M. Kao ◽  
W.A. Keller ◽  
S. Gleddie ◽  
G.G. Brown
Keyword(s):  
Plant Regeneration ◽  
Brassica Oleracea ◽  
Brassica Oleracea L

Microspore embryogenesis and plant regeneration in Brussels sprouts (Brassica oleracea L. var. gemmifera)

2015 ◽  
Vol 191 ◽  
pp. 31-37 ◽  
Author(s):  
Aisong Zeng ◽  
Yuanyuan Yan ◽  
Jiyong Yan ◽  
Lixiao Song ◽  
Bing Gao ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Brassica Oleracea ◽  
Microspore Embryogenesis ◽  
Brussels Sprouts ◽  
Brassica Oleracea L

High frequency plant regeneration from microspore-derived embryos of ornamental kale (Brassica oleracea L. var. acephala)

2011 ◽  
Vol 130 (1) ◽  
pp. 296-302 ◽  
Author(s):  
Yushu Wang ◽  
Yan Tong ◽  
Yuefei Li ◽  
Yun Zhang ◽  
Jun Zhang ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Brassica Oleracea ◽  
High Frequency ◽  
Ornamental Kale ◽  
Brassica Oleracea L

PRODUCTION OF ANTHER-DERIVED DIHAPLOID PLANTS IN AUTOTETRAPLOID MARROWSTEM KALE (BRASSICA OLERACEA VAR. ACEPHALA)

1981 ◽  
Vol 23 (2) ◽  
pp. 259-265 ◽  
Author(s):  
W. A. Keller ◽  
K. C. Armstrong
Keyword(s):  
High Temperature ◽  
Plant Regeneration ◽  
Brassica Oleracea ◽  
Microspore Embryogenesis ◽  
Potential Method ◽  
Stem Explants ◽  
Cytological Analysis ◽  
Hypocotyl Explants ◽  
Brassica Oleracea L

Microspore embryogenesis was induced in anthers of Brassica oleracea L. var. acephale D.C. cv. 'Polkow' (autotetraploid marrowstem kale) cultured under conditions similar to those previously developed for B. campestris and B. napus. Embryogenesis was stimulated by an initial high temperature culture treatment with maximal embryo yields obtained when anthers were cultured at 35 °C for one day prior to maintenance at 25 °C. Although most of the microspore-derived embryos failed to develop directly into plantlets, regeneration could be achieved from cultured hypocotyl explants. More than 60% of the regenerates had small, sterile flowers and cytological analysis of some of these plants revealed that they were dihaploids (2n = 2x = 18). Plant regeneration could be induced from dihaploid stem explants, thereby providing a potential method for propagation and maintenance of sterile material.

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