Factors influencing T-DNA transfer in Agrobacterium-mediated transformation of sugarbeet

1993 ◽  
Vol 12 (11) ◽  
Author(s):  
Benoit Jacq ◽  
Oliver Lesobre ◽  
RajbirS. Sangwan ◽  
BrigitteS. Sangwan-Norreel
Keyword(s):  
Dna Transfer ◽  
Agrobacterium Mediated Transformation ◽  
Factors Influencing

Factors Influencing T-DNA Transfer into Wheat and Barley Cells by Agrobacterium Tumefaciens

1998 ◽  
Vol 26 (1) ◽  
pp. 15-22 ◽  
Author(s):  
Guangqin Guo ◽  
Frank Maiwald ◽  
Petra Lorenzen ◽  
Hans-Henning Steinbiss
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Dna Transfer ◽  
Factors Influencing

scholarly journals Optimization of Agrobacterium-mediated transformation conditions of prospective genotypes of winter bread wheat by in planta method

2021 ◽  
Vol 28 ◽  
pp. 66-71
Author(s):  
O. V. Dubrovna ◽  
L. V. Slivka
Keyword(s):  
Transgenic Plants ◽  
Bread Wheat ◽  
Optical Density ◽  
Dna Transfer ◽  
Plant Genome ◽  
In Planta ◽  
Agrobacterium Mediated Transformation ◽  
The Third ◽  
Winter Bread Wheat ◽  
Inoculation Medium

Aim. Optimization of conditions for genetic transformation of new promising genotypes of winter bread wheat (T. aestivum L.) by in planta method. Methods. Agrobacterium-mediated transformation by in planta method using the strain AGL0 and vector construct pBi2E. Results. The influence of air temperature, optical density of cells of agrobacterial suspension, inoculation day and composition of inoculation medium on the frequency of obtaining transgenic plants of new winter wheat genotypes was studied. The dependence of the frequency obtaining of transgenic plants from environmental conditions, in particular temperature, has been established. It was found that the temperature regime of 20-22°C provided the largest number (4.8%) of wheat transformants, and when the temperature is reduced to 16-18°C there is a decrease in the efficiency of T-DNA transfer into the plant genome and the lowest frequency of transformation (0.7%). Conclusions. The largest number of transformants was obtained using a inoculation medium without sucrose, the optical density of cells of the agrobacterial suspension of 0.6 op.od. and inoculation on the third day after castration of ears. Keywords: T. aestivum, Agrobacterium-mediated transformation in planta, optimization of conditions.

Factors influencing Agrobacterium-mediated transformation of monocotyledonous species

2004 ◽  
Vol 40 (1) ◽  
pp. 31-45 ◽  
Author(s):  
Ming Cheng ◽  
Brenda A. Lowe ◽  
T. Michael Spencer ◽  
Xudong Ye ◽  
Charles L. Armstrong
Keyword(s):  
Agrobacterium Mediated Transformation ◽  
Factors Influencing

scholarly journals Factors Influencing Agrobacterium-Mediated Transformation Efficiency in Perennial Ryegrass

2009 ◽  
Vol 29 (3) ◽  
pp. 165-170 ◽  
Author(s):  
Ki-Won Lee ◽  
Ki-Yong Kim ◽  
Joung-Kyong Lee ◽  
Hyung-Soo Park ◽  
Kyung-Hee Kim ◽  
...  
Keyword(s):  
Perennial Ryegrass ◽  
Transformation Efficiency ◽  
Agrobacterium Mediated Transformation ◽  
Factors Influencing
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