Molecular cloning and expression of rat connexin40, a gap junction protein expressed in vascular smooth muscle

1992 ◽  
Vol 127 (1) ◽  
pp. 69-76 ◽  
Author(s):  
Eric C Beyer ◽  
Karen E Reed ◽  
Eileen M Westphale ◽  
H Lee Kanter ◽  
David M Larson
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Gap Junction ◽  
Molecular Cloning ◽  
Cloning And Expression ◽  
Junction Protein ◽  
Gap Junction Protein

Human Gap Junction Protein Connexin31: Molecular Cloning and Expression Analysis

1998 ◽  
Vol 248 (3) ◽  
pp. 910-915 ◽  
Author(s):  
Keirut Wenzel ◽  
Dieter Manthey ◽  
Klaus Willecke ◽  
Karl-Heinz Grzeschik ◽  
Otto Traub
Keyword(s):  
Gap Junction ◽  
Molecular Cloning ◽  
Expression Analysis ◽  
Cloning And Expression ◽  
Junction Protein ◽  
Gap Junction Protein

scholarly journals Reciprocal expression of connexin 40 and 45 during phenotypical changes in renin-secreting cells

2011 ◽  
Vol 300 (3) ◽  
pp. F743-F748 ◽  
Author(s):  
Birguel Kurt ◽  
Lisa Kurtz ◽  
Maria L. Sequeira-Lopez ◽  
R. Ariel Gomez ◽  
Klaus Willecke ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Gap Junction ◽  
Vascular Smooth Muscle Cells ◽  
Cell Lineage ◽  
Muscle Cells ◽  
Sodium Deficiency ◽  
Junction Protein ◽  
Gap Junction Protein

Gap junctional coupling of renin-producing cells is of major functional importance for the control of renin synthesis and release. This study was designed to determine the relevance of the vascular gap junction protein connexin 45 (Cx45) for the control of renin expression and secretion. By crossbreeding mice which drive Cre recombinase under the control of the endogenous renin promoter with mice harboring floxed Cx45 gene alleles, we generated viable mice with a deletion of Cx45 in the renin cell lineage. These mice were normotensive, and renin cells in their kidneys were normal with regard to localization and number. Sodium deficiency induced typical recruitment of renin-producing cells along afferent arterioles, whereas sodium overload resulted in a decrease in the number of cells expressing renin. Regulation of renin secretion by perfusion pressure, catecholamines, and angiotensin II from isolated kidneys of mice with renin cell-specific deletion of Cx45 was normal. Analyzing Cx45 promoter activity in cells of the preglomerular arteriolar tree by using mice driving the reporter gene LacZ under the control of the Cx45 promoter revealed strong staining in smooth muscle cells of the media, whereas renin-expressing cells were almost devoid of LacZ staining. Conversely, renin-producing cells, but not vascular smooth muscle cells expressed the gap junction protein Cx40. These findings suggest that Cx45 plays no major functional role in renin-producing cells, probably because the expression of Cx45 is downregulated in these cells. Since renin-producing cells in the adult kidney can reversibly transform into vascular smooth muscle cells and vice versa, our findings on connexin expression indicate that these phenotype switches are paralleled by characteristic reciprocal changes in the transcriptional activity of Cx40 and Cx45 genes.

scholarly journals Chick connexin-56, a novel lens gap junction protein. Molecular cloning and functional expression.

1993 ◽  
Vol 268 (1) ◽  
pp. 706-712
Author(s):  
D.M. Rup ◽  
R.D. Veenstra ◽  
H.Z. Wang ◽  
P.R. Brink ◽  
E.C. Beyer
Keyword(s):  
Gap Junction ◽  
Molecular Cloning ◽  
Functional Expression ◽  
Junction Protein ◽  
Gap Junction Protein

scholarly journals Molecular cloning, expression analysis, and functional characterization of connexin44.1: A zebrafish lens gap junction protein

2001 ◽  
Vol 221 (2) ◽  
pp. 238-247 ◽  
Author(s):  
Nicola Cason ◽  
Thomas W. White ◽  
Shaohong Cheng ◽  
Daniel A. Goodenough ◽  
Gunnar Valdimarsson
Keyword(s):  
Gap Junction ◽  
Molecular Cloning ◽  
Expression Analysis ◽  
Functional Characterization ◽  
Junction Protein ◽  
Gap Junction Protein

scholarly journals Molecular cloning and functional expression of human connexin37, an endothelial cell gap junction protein.

1993 ◽  
Vol 91 (3) ◽  
pp. 997-1004 ◽  
Author(s):  
K E Reed ◽  
E M Westphale ◽  
D M Larson ◽  
H Z Wang ◽  
R D Veenstra ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Gap Junction ◽  
Molecular Cloning ◽  
Functional Expression ◽  
Junction Protein ◽  
Gap Junction Protein
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