Agrobacterium-mediated transformation and regeneration of fertile transgenic plants of chinese cabbage (brassica campestris ssp. pekinensis cv. ‘spring flavor’)

1995 ◽  
Vol 14 (10) ◽  
pp. 620-625 ◽  
Author(s):  
Se Jun ◽  
Seok Yoon Kwon ◽  
Kee Yoeup Pack ◽  
Kyung-Hee Paek
Keyword(s):  
Transgenic Plants ◽  
Chinese Cabbage ◽  
Brassica Campestris ◽  
Agrobacterium Mediated Transformation

Fertility and Cytomorphology of Anthers in New Cytoplasmic Male-Sterile Lines of Chinese Cabbage(Brassica campestris L. ssp. pekinensis)

2012 ◽  
Vol 30 (1) ◽  
pp. 49
Author(s):  
Zhan SHI ◽  
Zheng-Jie WAN ◽  
Yue-Jin XU ◽  
Rui-Chang ZOU ◽  
Tao HUANG ◽  
...  
Keyword(s):  
Chinese Cabbage ◽  
Brassica Campestris ◽  
Male Sterile ◽  
Cytoplasmic Male Sterile

scholarly journals Citrus Cell Suspension Culture Establishment, Maintenance, Efficient Transformation and Regeneration to Complete Transgenic Plant

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 664
Author(s):  
M. Moniruzzaman ◽  
Yun Zhong ◽  
Zhifeng Huang ◽  
Huaxue Yan ◽  
Lv Yuanda ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Culture System ◽  
Transformation Rate ◽  
Suspension Cell Culture ◽  
Citrus Reticulata ◽  
Agrobacterium Mediated Transformation ◽  
Solid Media

Agrobacterium-mediated transformation of epicotyl segment has been used in Citrus transgenic studies. The approach suffers, however, from limitations such as occasionally seed unavailability, the low transformation efficiency of juvenile tissues and the high frequency of chimeric plants. Therefore, a suspension cell culture system was established and used to generate transgenic plants in this study to overcome the shortcomings. The embryonic calli were successfully developed from undeveloped ovules of the three cultivars used in this study, “Sweet orange”-Egyptian cultivar (Citrus sinensis), “Shatangju” (Citrus reticulata) and “W. Murcott” (Citrus reticulata), on three different solid media. Effects of media, genotypes and ages of ovules on the induction of embryonic calli were also investigated. The result showed that the ovules’ age interferes with the callus production more significantly than media and genotypes. The 8 to 10 week-old ovules were found to be the best materials. A cell suspension culture system was established in an H+H liquid medium. Transgenic plants were obtained from Agrobacterium-mediated transformation of cell suspension as long as eight weeks subculture intervals. A high transformation rate (~35%) was achieved by using our systems, confirming BASTA selection and later on by PCR confirmation. The results demonstrated that transformation of cell suspension should be more useful for the generation of non-chimeric transgenic Citrus plants. It was also shown that our cell suspension culture procedure was efficient in maintaining the vigor and regeneration potential of the cells.

scholarly journals Receiving of genetic-modified wheat plants with heterolous ornitin-δ-aminotransferase gene

2018 ◽  
Vol 22 ◽  
pp. 222-227
Author(s):  
O. M. Honcharuk ◽  
O. V. Dubrovna
Keyword(s):  
Triticum Aestivum ◽  
Transgenic Plants ◽  
Bread Wheat ◽  
Binary Vector ◽  
Triticum Aestivum L ◽  
Callus Cultures ◽  
Pcr Analysis ◽  
Genetic Construct ◽  
Agrobacterium Mediated Transformation

Aim. Receiving of genetically modified plants of bread wheat with heterologous ornithine‑δ‑aminotransferase gene. Methods. Agrobacterium-mediated transformation of callus cultures in vitro, PCR-analysis. Results. By Agrobacterium-mediated transformation of the morphogenic calluses of bread wheat (Triticum aestivum L.) using the AGLO strain containing the binary vector pBi-OAT with the target ornithine-δ-aminotransferase (oat) and selective neomycinphosphotransferase II (nptII), transgenic plants-regenerators have been obtained. Conclusions. As a result of the genetic transformation of Zimoyarka variety, 12 wheat regenerants were obtained in the genome which revealed a complete integration of the genetic construct containing the oat and nptII transgenes. Keywords: Triticum aestivum L., Agrobacterium-mediated transformation, ornithine‑δ‑aminotransferase gene, PCR-analysis.

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