Plant regeneration through somatic embryogenesis from callus induced on immature embryos of Alstroemeria spp. L.

1996 ◽  
Vol 15 (5) ◽  
pp. 377-380 ◽  
Author(s):  
Carla E. Van Schaik ◽  
A. Posthuma ◽  
M. J. De Jeu ◽  
E. Jacobsen
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Immature Embryos

scholarly journals Efficient Somatic Embryogenesis and Plant Regeneration from Immature Embryos of Tapiscia sinensis Oliv., an Endemic and Endangered Species in China

HortScience ◽  
2014 ◽  
Vol 49 (12) ◽  
pp. 1558-1562 ◽  
Author(s):  
Yuyu Wang ◽  
Faju Chen ◽  
Yubing Wang ◽  
Xiaoling Li ◽  
Hongwei Liang
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Activated Charcoal ◽  
Somatic Embryos ◽  
Basal Medium ◽  
Immature Embryos ◽  
Zygotic Embryos ◽  
Naphthaleneacetic Acid ◽  
Embryogenic Calli ◽  
Induction Rate

High-frequency somatic embryogenesis and plant regeneration were achieved from immature cotyledonary-stage embryos in the endangered plant, Tapiscia sinensis Oliv. Plant growth regulators with different concentrations and combinations on embryogenesis capacity were studied. The optimal explants for in vitro somatic embryogenesis were immature embryos in T. sinensis. A high callus induction rate of 100% was achieved on Murashige and Skoog (MS) basal medium supplemented with 1.0 mg·Ll−1 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5% (w/v) activated charcoal. Alternatively, a high induction rate (96.16%) of somatic embryogenesis was obtained on MS basal medium supplemented with the combination of 0.05 mg·L−1 α-naphthaleneacetic acid (NAA) and 0.2 mg·L−1 6-benzylaminopurine (6-BA), and somatic embryos proliferated fastest on the mentioned medium supplemented with 0.5% (w/v) activated charcoal and 3% (w/v) sucrose, inoculation of explants proliferating 21 times in the 23-day subculture. Of the 100 plantlets transferred to field after the acclimation, 95 (95%) survived. Based on the histocytological observations, the development of somatic embryos was similar to that of zygotic embryos. There were two accumulation peaks of starch grains in the embryogenic calli and in the globular-stage embryos, both closely related to the energy supply, and the embryoids were of multicelluar origin.

Plant regeneration through somatic embryogenesis from callus induced on immature embryos of Alstroemeria spp. L.

1996 ◽  
Vol 15 (5) ◽  
pp. 377-380 ◽  
Author(s):  
Carla E. Van Schaik ◽  
A. Posthuma ◽  
M.J. De Jeu ◽  
E. Jacobsen
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Immature Embryos
Sign in / Sign up

Export Citation Format

Share Document