Diplotaxis catholica + Brassica juncea somatic hybrids: molecular and cytogenetic characterization

1995 ◽  
Vol 14 (9) ◽  
Author(s):  
P.B. Kirti ◽  
T. Mohapatra ◽  
H. Khanna ◽  
S. Prakash ◽  
V.L. Chopra
Keyword(s):  
Brassica Juncea ◽  
Somatic Hybrids ◽  
Cytogenetic Characterization ◽  
Diplotaxis Catholica

Somatic hybrids between Brassica juncea (L). Czern. and Diplotaxis harra (Forsk.) Boiss and the generation of backcross progenies

1995 ◽  
Vol 91-91 (6-7) ◽  
pp. 1167-1172 ◽  
Author(s):  
F. Begum ◽  
S. Paul ◽  
N. Bag ◽  
S. R. Sikdar ◽  
S. K. Sen
Keyword(s):  
Brassica Juncea ◽  
Somatic Hybrids

Cytogenetical and molecular investigations on somatic hybrids of Sinapis alba and Brassica juncea and their backcross progeny

1996 ◽  
Vol 115 (6) ◽  
pp. 480-483 ◽  
Author(s):  
K. Gaikwad ◽  
P. B. Kirti ◽  
A. Sharma ◽  
S. Prakash ◽  
V. L. Chopra
Keyword(s):  
Brassica Juncea ◽  
Somatic Hybrids ◽  
Backcross Progeny ◽  
Sinapis Alba

scholarly journals Molecular and Cytogenetic Characterization of Triploid Somatic Hybrids between 'Shogun' Mandarin and Grapefruit.

2002 ◽  
Vol 19 (5) ◽  
pp. 345-352 ◽  
Author(s):  
Hisato KUNITAKE ◽  
Kohji NAGASAWA ◽  
Kayo TAKAMI ◽  
Haruki KOMATSU
Keyword(s):  
Somatic Hybrids ◽  
Cytogenetic Characterization

Production and characterization of intergeneric somatic hybrids of Trachystoma ballii and Brassica juncea

1992 ◽  
Vol 11 (2) ◽  
Author(s):  
P.B. Kirti ◽  
S.B. Narasimhulu ◽  
Shyam Prakash ◽  
V.L. Chopra
Keyword(s):  
Brassica Juncea ◽  
Somatic Hybrids

Cytogenetic characterization and fae1 gene variation in progenies from asymmetric somatic hybrids between Brassica napus and Crambe abyssinica

Genome ◽  
2004 ◽  
Vol 47 (4) ◽  
pp. 724-731 ◽  
Author(s):  
Y P Wang ◽  
R J Snowdon ◽  
E Rudloff ◽  
P Wehling ◽  
W Friedt ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Somatic Hybrids ◽  
Genetic Material ◽  
Asymmetric Somatic Hybrids ◽  
Crambe Abyssinica ◽  
Gene Variation ◽  
Cytogenetic Characterization ◽  
Sexual Progeny ◽  
Chromatin Bridges

Sexual progenies of asymmetric somatic hybrids between Brassica napus and Crambe abyssinica were analyzed with respect to chromosomal behavior, fae1 gene introgression, fertility, and fatty-acid composition of the seed. Among 24 progeny plants investigated, 11 plants had 38 chromosomes and were characterized by the occurrence of normal meiosis with 19 bivalents. The other 13 plants had more than 38 chromosomes, constituting a complete chromosomal set from B. napus plus different numbers of additional chromosomes from C. abyssinica. The chromosomes of B. napus and C. abyssinica origin could be clearly discriminated by genomic in situ hybridization (GISH) in mitotic and meiotic cells. Furthermore, meiotic GISH enabled identification of intergenomic chromatin bridges and of asynchrony between the B. napus and C. abyssinca meiotic cycles. Lagging, bridging and late disjunction of univalents derived from C. abyssinica were observed. Analysis of cleaved amplified polymorphic sequence (CAPS) markers derived from the fae1 gene showed novel patterns different from the B. napus recipient in some hybrid offspring. Most of the progeny plants had a high pollen fertility and seed set, and some contained significantly greater amounts of seed erucic acid than the B. napus parent. This study demonstrates that a part of the C. abyssinica genome can be transferred into B. napus via asymmetric hybridization and maintained in sexual progenies of the hybrids. Furthermore, it confirms that UV irradiation improves the fertility of the hybrid and of its sexual progeny via chromosomal elimination and facilitates the introgression of exotic genetic material into crop species.Key words: asymmetric somatic hybrid, Brassica napus, Crambe abyssinica, cytogenetics, fae1 gene.

Comparative karyotype analysis of threePassifloraL. species and cytogenetic characterization of somatic hybrids

Caryologia ◽  
2005 ◽  
Vol 58 (3) ◽  
pp. 220-228 ◽  
Author(s):  
M. Cuco Silvia ◽  
Maria Lucia C. Vieira ◽  
Mateus Mondin ◽  
Margarida L. R. Aguiar-Perecin
Keyword(s):  
Somatic Hybrids ◽  
Karyotype Analysis ◽  
Cytogenetic Characterization
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