Further evidence of a cybridization requirement for plant regeneration from citrus leaf protoplasts following somatic fusion

1996 ◽  
Vol 15 (9) ◽  
pp. 672-676 ◽  
Author(s):  
J. W. Grosser ◽  
F. G. Gmitter ◽  
N. Tusa ◽  
G. Reforgiato Recupero ◽  
P. Cucinotta
Keyword(s):  
Plant Regeneration ◽  
Citrus Leaf ◽  
Somatic Fusion ◽  
Leaf Protoplasts

Further evidence of a cybridization requirement for plant regeneration from citrus leaf protoplasts following somatic fusion

1996 ◽  
Vol 15 (9) ◽  
pp. 672-676 ◽  
Author(s):  
G. Reforgiato Recupero ◽  
P. Cucinotta ◽  
N. Tusa ◽  
J.W. Grosser ◽  
F.G. Gmitter Jr.
Keyword(s):  
Plant Regeneration ◽  
Citrus Leaf ◽  
Somatic Fusion ◽  
Leaf Protoplasts

Plant regeneration from in vitro -cultured seedling leaf protoplasts of Actinidia eriantha Benth

1998 ◽  
Vol 17 (10) ◽  
pp. 819-821 ◽  
Author(s):  
Y.-J. Zhang ◽  
Y.-Q. Qian ◽  
X.-J. Mu ◽  
Q.-G. Cai ◽  
Y.-L. Zhou ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Seedling Leaf ◽  
Leaf Protoplasts

Isolation, Culture, and Plant Regeneration From Leaf Protoplasts of Passiflora

2005 ◽  
pp. 201-210
Author(s):  
Michael R. Davey ◽  
Paul Anthony ◽  
J. Brian Power ◽  
Kenneth C. Lowe
Keyword(s):  
Plant Regeneration ◽  
Leaf Protoplasts

Plant regeneration from leaf protoplasts of evening primrose ( Oenothera hookeri )

1998 ◽  
Vol 17 (8) ◽  
pp. 601-604 ◽  
Author(s):  
N. Kuchuk ◽  
R. G. Herrmann ◽  
H.-U. Koop
Keyword(s):  
Plant Regeneration ◽  
Evening Primrose ◽  
Oenothera Hookeri ◽  
Leaf Protoplasts

Plant regeneration from leaf protoplasts of six tomato cultivars

Plant Science ◽  
1985 ◽  
Vol 39 (3) ◽  
pp. 199-204 ◽  
Author(s):  
Randall P. Niedz ◽  
Sara M. Rutter ◽  
Levis W. Handley ◽  
Kenneth C. Sink
Keyword(s):  
Plant Regeneration ◽  
Tomato Cultivars ◽  
Leaf Protoplasts

Plant regeneration from leaf protoplasts of Solanum torvum

1987 ◽  
Vol 6 (4) ◽  
pp. 302-304 ◽  
Author(s):  
A. Guri ◽  
M. Volokita ◽  
K. C. Sink
Keyword(s):  
Plant Regeneration ◽  
Solanum Torvum ◽  
Leaf Protoplasts

scholarly journals Isolation and culture of leaf protoplasts from Tunisian grapes

OENO One ◽  
2003 ◽  
Vol 37 (3) ◽  
pp. 145 ◽  
Author(s):  
Ahmed Mliki ◽  
Rahma Jardak ◽  
Götz M. Reustle ◽  
Abdelwahed Ghorbel
Keyword(s):  
Cell Division ◽  
Plant Regeneration ◽  
Aspergillus Niger ◽  
Shoot Culture ◽  
Protoplast Isolation ◽  
Penicillium Funiculosum ◽  
Experimental Conditions ◽  
Grape Varieties ◽  
Leaf Protoplasts

<p style="text-align: justify;">Experimental conditions for leaf protoplast isolation and culture were optimised for <em>in vitro</em> plants deriving from shoot culture of two Tunisian grape varieties, Sakasly and Muscat d’Alexandrie (<em>Vitis vinifera</em> L.). The best yields were obtained from leaves of 4 to 5 weeks old <em>in vitro</em> plants, digested for 13 hours under 25 rpm agitation with an enzymatic mixture containing 0.25 % cellulase of <em>Aspergillus niger</em>, 0.25 % cellulase of <em>Penicillium funiculosum</em>, 0.5 % cellulysin of <em>Trichoderma viridae</em>, and 0.2 % macerozyme R-10 of <em>Rhizopus</em> sp. More than 50 % of the purified protoplasts had a diameter of 30-40 μm and were rich in chloroplasts. Best aptitude for cell division was found in protoplasts immobilised in sodium alginate layers at a density of 0.5x10<sup>6</sup> cell/ml, cultivated in CPW-13 medium containing 4 mg/l of NOA and 0.88 mg/l of TDZ. The variety Muscat d’Alexandrie gave better yield whereas Sakasly showed better cell division rates. Formation of micro and macrocallus have been obtained, but the oxidation of the medium has to be solved in order to promote plant regeneration.</p>

Sign in / Sign up

Export Citation Format

Share Document