This work describes the identification of components in the Cap-binding complexes in non-germinated and 24-h-imbibed seeds using mass spectrometry. This approach revealed new components particularly present in the non-germinated seed. Among these, two heat shock proteins, HSP101 and HSP70, were detected as well as several proteins involved in carbohydrate metabolism. Between the new components of maize Cap-binding complexes, several proteins contain a motif that identifies them as potential direct interactors with eIF4E or eIF(iso)4E.Together with the major abundance of eIF(iso)4E at this developmental stage, our findings indicate clear differences between the translation initiation complexes that are available for protein synthesis right upon water imibition and those that are present once germination has been completed.
Heat Shock Protein Synthesis Induced by Methomyl in Maize (Zea mays L.) Seedlings