Agrobacterium tumefaciens-mediated transformation of Artemisia annua L. and regeneration of transgenic plants

1996 ◽  
Vol 15 (12) ◽  
pp. 929-933 ◽  
Author(s):  
Annemieke Vergauwe ◽  
Ronny Cammaert ◽  
Dirk Vandenberghe ◽  
Christiane Genetello ◽  
Dirk Inze ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Transgenic Plants ◽  
Artemisia Annua ◽  
Artemisia Annua L

Agrobacterium tumefaciens?mediated transformation of Artemisia annua L. and regenration of transgenic plants

1996 ◽  
Vol 15 (12) ◽  
pp. 929-933 ◽  
Author(s):  
Annemieke Vergauwe ◽  
Rony Cammaert ◽  
Dirk Vanderberghe ◽  
Christiane Genetello ◽  
Dirk Inze ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Transgenic Plants ◽  
Artemisia Annua ◽  
Artemisia Annua L

Factors influencing Agrobacterium tumefaciens-mediated transformation of Artemisia annua L.

1998 ◽  
Vol 18 (1-2) ◽  
pp. 105-110 ◽  
Author(s):  
A. Vergauwe ◽  
E. Van Geldre ◽  
D. Inzé ◽  
M. Van Montagu ◽  
E. Van den Eeckhout
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Artemisia Annua ◽  
Artemisia Annua L ◽  
Factors Influencing

scholarly journals An improved Agrobacterium tumefaciens mediated transformation of Artemisia annua L. by using stem internodes as explants

2013 ◽  
Vol 49 (No. 3) ◽  
pp. 123-129 ◽  
Author(s):  
N. Tian ◽  
S. Liu ◽  
H. Ting ◽  
J. Huang ◽  
S. van der Krol ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Optical Density ◽  
Culture Medium ◽  
Root Formation ◽  
Transformation Efficiency ◽  
Artemisia Annua ◽  
Adventitious Shoot ◽  
Selective Medium ◽  
Artemisia Annua L ◽  
Low Efficiency

Transformation of Artemisia annua, which produces the sesquiterpenoid endoperoxide artemisinin widely used for the treatment of malaria, has been hampered by the low efficiency of adventitious shoot and root formation on a selective medium containing additional compounds for Agrobacterium decontamination. Here we identified several factors which were all shown to be of importance for optimization of Artemisia annua transformation. Results indicated that stem internodes showed better resistance capacity to Agrobacterium decontaminator than leaves did. Agrobacterium tumefaciens with an optical density (OD) value of 0.2–0.5 plus 100 μmol of acetosyringone per litre of solution gave the best transformation efficiency. Moreover, kanamycin at 30 mg/l in the culture medium was effective in suppressing the growth of non-transformed tissue. Furthermore, transgenic shoots required an early induction of rooting. In addition, dimethyl sulphoxide considerably improved the rooting of shoots. The present work provides rapid and reproducible transformation and regeneration of A. annua.

Comparative phytochemical study on the phenolic compounds content of Artemisia annua L. from wild populations in Republic of Moldova

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
CP Stefanache ◽  
OC Bujor ◽  
R Necula ◽  
V Ghendov ◽  
A Trifan ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Artemisia Annua ◽  
Wild Populations ◽  
Phytochemical Study ◽  
Republic Of Moldova ◽  
Artemisia Annua L

T-DNA integration patterns in transgenic plants mediated by Agrobacterium tumefaciens

2011 ◽  
Vol 33 (12) ◽  
pp. 1327-1334 ◽  
Author(s):  
Lin YANG ◽  
Feng-Ling FU ◽  
Wan-Chen LI
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Transgenic Plants ◽  
Dna Integration
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