Processing in vitro of pronapin, the 2S storage-protein precursor of Brassica napus produced in a baculovirus expression system

Planta ◽  
1996 ◽  
Vol 200 (4) ◽  
Author(s):  
Eva Muren ◽  
Lars Rask
Keyword(s):  
Brassica Napus ◽  
Storage Protein ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Protein Precursor ◽  
Baculovirus Expression

Human β nerve growth factor obtained from a baculovirus expression system has potent in vitro and in vivo neurotrophic activity

1990 ◽  
Vol 110 (1) ◽  
pp. 11-24 ◽  
Author(s):  
Jim Barnett ◽  
Preston Baecker ◽  
Carol Routledge-Ward ◽  
Hela Bursztyn-Pettegrew ◽  
Joan Chow ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Nerve Growth ◽  
Neurotrophic Activity ◽  
Baculovirus Expression

scholarly journals Expression of functional G protein beta gamma dimers of defined subunit composition using a baculovirus expression system.

1992 ◽  
Vol 267 (19) ◽  
pp. 13123-13126 ◽  
Author(s):  
S.G. Graber ◽  
R.A. Figler ◽  
V.K. Kalman-Maltese ◽  
J.D. Robishaw ◽  
J.C. Garrison
Keyword(s):  
G Protein ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Subunit Composition ◽  
Baculovirus Expression

scholarly journals Production, processing and partial purification of functional G protein βγ subunits in baculovirus-infected insect cells

1992 ◽  
Vol 286 (3) ◽  
pp. 677-680 ◽  
Author(s):  
J D Robishaw ◽  
V K Kalman ◽  
K L Proulx
Keyword(s):  
G Protein ◽  
G Proteins ◽  
Insect Cells ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Partial Purification ◽  
Baculovirus Expression ◽  
Gamma Subunits ◽  
Infected Insect ◽  
Beta 2

As a result of the inability to resolve the heterogeneous mixture of G protein beta gamma subunits present in tissues, it has not been possible to compare different beta gamma subunits of the G proteins in terms of their proposed roles in receptor-effector coupling. This study was undertaken to establish the utility of the baculovirus expression system in producing homogeneous beta gamma subunits of defined composition for the comparative analysis of these subunits in reconstitution systems. In this study we report the expression, and appropriate post-translational processing, of recombinant beta 2, gamma 2 and gamma 3 subunits. In addition, we show that the recombinant beta gamma subunits can be readily purified, and can functionally interact with the alpha subunits of the G proteins.

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