Survival at 20� C and cryopreservation of isolated sperm cells from Zea mays pollen grains

1993 ◽  
Vol 6 (3) ◽  
Author(s):  
P. Roeckel ◽  
C. Dumas
Keyword(s):  
Zea Mays ◽  
Pollen Grains ◽  
Sperm Cells

Morphometric analysis of isolated Zea mays sperm

1989 ◽  
Vol 93 (1) ◽  
pp. 179-184
Author(s):  
VINCENT T. WAGNER ◽  
CHRISTIAN DUMAS ◽  
H. LLOYD MOGENSEN
Keyword(s):  
Zea Mays ◽  
Morphometric Analysis ◽  
Pollen Grains ◽  
Sperm Cells ◽  
Higher Plant ◽  
Average Cell ◽  
Developmental States ◽  
Surface Areas ◽  
Male Gametes ◽  
Biochemical Analyses

Recently developed techniques for the isolation of sex cells of flowering plants represent an important first step toward eventual detailed cytological and biochemical analyses and experimental manipulations of higher plant gametes that was not previously possible. In the present investigation we have analysed a population of isolated viable male gametes from the standpoint of qualitative and quantitative ultrastructure in order to elucidate the process of double fertilization in angiosperms. The vast majority of the Zea mays sperm cells were found to be structurally intact spherical cells. Morphometric analysis of 400 isolated sperm cells indicates that the average cell is 7.66 μm in diameter, has volume and surface areas of 235.3μm3 and 184.3μm2, respectively, and contains a variously shaped, often curved nucleus, which occupies 38.5% of the cell volume. Other cell constituents and their relative volumes (expressed as percentages of the protoplasm) included in the analysis are: vacuoles, 9.3%; endoplasmic lamellae, 5.9%; mitochondria, 3.5%; osmiophilic bodies, 1.2%; Golgi complex, 0.6%; and hyaloplasm (back-ground cytoplasm), 41.1%. No plastids or microtubules were observed in the hybrid line used. Two types of nuclei were found, i.e. heterochromatic and non-heterochromatic. Although statistical analysis of cell components shows some significant differences between cells of the two nuclear types, we do not believe that they represent the two sperms of a pair within pollen grains, since they do not occur in a 1:1 ratio. It is more likely that the variability within the sperm population is the result of slightly different developmental states at the time of anther dehiscence.

scholarly journals Procedure to Isolate Viable Sperm Cells from Corn (Zea mays L.) Pollen Grains

1987 ◽  
Vol 85 (4) ◽  
pp. 876-878 ◽  
Author(s):  
Isabelle Dupuis ◽  
Patricia Roeckel ◽  
Elisabeth Matthys-Rochon ◽  
Christian Dumas
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Pollen Grains ◽  
Sperm Cells ◽  
Viable Sperm

Isolation and Characterization of Viable Sperm Cells from Tricellular Pollen Grains

1988 ◽  
pp. 245-250 ◽  
Author(s):  
E. Matthys-Rochon ◽  
S. Detchepare ◽  
V. Wagner ◽  
P. Roeckel ◽  
C. Dumas
Keyword(s):  
Pollen Grains ◽  
Sperm Cells ◽  
Isolation And Characterization ◽  
Viable Sperm

Fine structure of sperm cells in pollen grains ofBeta

PROTOPLASMA ◽  
1969 ◽  
Vol 68 (1-2) ◽  
pp. 237-240 ◽  
Author(s):  
Lynn L. Hoefert
Keyword(s):  
Fine Structure ◽  
Pollen Grains ◽  
Sperm Cells

Effects of calcium, magnesium, potassium and boron on sperm cells isolated from pollen of Zea mays L.

1995 ◽  
Vol 8 (2) ◽  
Author(s):  
G. Zhang ◽  
C.M. Williams ◽  
M.K. Campenot ◽  
L.E. McGann ◽  
A.J. Cutler ◽  
...  
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Sperm Cells ◽  
Calcium Magnesium

Development of sperm cells in barley

1975 ◽  
Vol 53 (10) ◽  
pp. 1051-1062 ◽  
Author(s):  
David D. Cass ◽  
Ilana Karas
Keyword(s):  
Cell Membrane ◽  
Vegetative Cell ◽  
Generative Cell ◽  
Pollen Grains ◽  
Complete Separation ◽  
Pollen Wall ◽  
Vegetative Nucleus ◽  
Sperm Cells ◽  
Subsequent Stage ◽  
Microspore Stage

Ultrastructural events in barley sperm development were examined from the uninucleate microspore stage to establishment of two mature sperm cells in pollen grains. Microspore mitosis produces a vegetative nucleus and a naked generative cell, both embedded in vegetative cell cytoplasm. The generative cell membrane is enclosed by vegetative cell membrane. The generative cell, at first apparently unattached, becomes attached to the pollen wall and acquires a cell wall by centripetal vesicle accumulation. Wall formation may be complete at the time of generative cell karyokinesis; karyokinesis occurs while the generative cell is attached to the pollen wall. Cytokinesis of the generative cell is delayed. The subsequent stage is a binucleate, attached generative cell with a wall. Generative cell cytokinesis appears to involve formation of a partition between the two sperm nuclei. Eventual complete separation of the sperm cells occurs only after the two-celled derivative of the generative cell detaches from the pollen wall. Final stages in sperm cell separation are considered to result from degradation of the partitioning and surrounding wall, not from furrowing of a naked binucleate generative cell according to previous suggestions. Mature plastids were not observed in the generative cell or the sperms.

Ultraviolet irradiation of maize (Zea Mays L.) pollen grains

1977 ◽  
Vol 49 (6) ◽  
pp. 253-258 ◽  
Author(s):  
P. L. Pfahler ◽  
H. F. Linskens
Keyword(s):  
Zea Mays ◽  
Ultraviolet Irradiation ◽  
Zea Mays L ◽  
Pollen Grains

scholarly journals Sperm cells of Zea mays have a complex complement of mRNAs

2003 ◽  
Vol 34 (5) ◽  
pp. 697-707 ◽  
Author(s):  
Michele L. Engel ◽  
Annie Chaboud ◽  
Christian Dumas ◽  
Sheila McCormick
Keyword(s):  
Zea Mays ◽  
Sperm Cells

scholarly journals KAKU4-mediated deformation of the vegetative nucleus controls its precedent migration over sperm cells in pollen tubes

2019 ◽  
Author(s):  
Chieko Goto ◽  
Kentaro Tamura ◽  
Satsuki Nishimaki ◽  
Naoki Yanagisawa ◽  
Kumi Matsuura-Tokita ◽  
...  
Keyword(s):  
Competitive Ability ◽  
Pollen Grains ◽  
Nuclear Lamina ◽  
Pollen Tubes ◽  
Vegetative Nucleus ◽  
Sperm Cells ◽  
Wild Type ◽  
Cell Nuclei ◽  
In The Wild ◽  
Dense Accumulation

AbstractA putative nuclear lamina protein, KAKU4, modulates nuclear morphology in Arabidopsis thaliana seedlings but its physiological significance is unknown. KAKU4 was strongly expressed in mature pollen grains, each of which has a vegetative cell and two sperm cells. KAKU4 protein was highly abundant on the envelopes of vegetative nuclei (VNs) and less abundant on the envelopes of sperm cell nuclei (SCNs) in pollen grains and elongating pollen tubes. VN is irregularly shaped in wild-type pollen. However, KAKU4 deficiency caused it to become more spherical. These results suggest that the dense accumulation of KAKU4 is responsible for the irregular shape of the VNs. After a pollen grain germinates, the VN and SCNs migrate to the tip of the pollen tube. In the wild type, the VN preceded the SCNs in 91–93% of the pollen tubes, whereas in kaku4 mutants, the VN trailed the SCNs in 39–58% of the pollen tubes. kaku4 pollen was less competitive than wild-type pollen after pollination, although it had an ability to fertilize. Taken together, our results suggest that controlling the nuclear shape in vegetative cells of pollen grains by KAKU4 ensures the orderly migration of the VN and sperm cells in pollen tubes.HighlightThe nuclear envelope protein KAKU4 is involved in controlling the migration order of vegetative nuclei and sperm cells in pollen tubes, affecting the competitive ability of pollen for fertilization.

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