Effects of gamma-aminobutyric acid receptor agonists and antagonist on LHRH-synthesizing neurons as detected by immunocytochemistry and in situ hybridization

1991 ◽  
Vol 87 (1) ◽  
Author(s):  
H.T. Bergen ◽  
J.F. Hejtmancik ◽  
D.W. Pfaff
Keyword(s):  
In Situ Hybridization ◽  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid ◽  
Acid Receptor ◽  
Receptor Agonists

Synapsin I Synchronizes GABA Release in Distinct Interneuron Subpopulations

2019 ◽  
Vol 30 (3) ◽  
pp. 1393-1406
Author(s):  
N Forte ◽  
F Binda ◽  
A Contestabile ◽  
F Benfenati ◽  
P Baldelli
Keyword(s):  
In Situ Hybridization ◽  
Patch Clamp ◽  
Action Potential ◽  
Hippocampal Slices ◽  
Gaba Release ◽  
Aminobutyric Acid ◽  
Synapsin I ◽  
Gamma Aminobutyric Acid ◽  
Asynchronous Release

Abstract Neurotransmitters can be released either synchronously or asynchronously with respect to action potential timing. Synapsins (Syns) are a family of synaptic vesicle (SV) phosphoproteins that assist gamma-aminobutyric acid (GABA) release and allow a physiological excitation/inhibition balance. Consistently, deletion of either or both Syn1 and Syn2 genes is epileptogenic. In this work, we have characterized the effect of SynI knockout (KO) in the regulation of GABA release dynamics. Using patch-clamp recordings in hippocampal slices, we demonstrate that the lack of SynI impairs synchronous GABA release via a reduction of the readily releasable SVs and, in parallel, increases asynchronous GABA release. The effects of SynI deletion on synchronous GABA release were occluded by ω-AgatoxinIVA, indicating the involvement of P/Q-type Ca2+channel-expressing neurons. Using in situ hybridization, we show that SynI is more expressed in parvalbumin (PV) interneurons, characterized by synchronous release, than in cholecystokinin or SOM interneurons, characterized by a more asynchronous release. Optogenetic activation of PV and SOM interneurons revealed a specific reduction of synchronous release in PV/SynIKO interneurons associated with an increased asynchronous release in SOM/SynIKO interneurons. The results demonstrate that SynI is differentially expressed in interneuron subpopulations, where it boosts synchronous and limits asynchronous GABA release.

scholarly journals Effect of Androgens on Sexual Differentiation of Pituitary Gamma-Aminobutyric Acid Receptor Subunit GABAB Expression

2004 ◽  
Vol 80 (3) ◽  
pp. 129-142 ◽  
Author(s):  
María S. Bianchi ◽  
Paolo N. Catalano ◽  
María M. Bonaventura ◽  
Patricia Silveyra ◽  
Bernhard Bettler ◽  
...  
Keyword(s):  
Sexual Differentiation ◽  
Aminobutyric Acid ◽  
Receptor Subunit ◽  
Gamma Aminobutyric Acid ◽  
Acid Receptor
Sign in / Sign up

Export Citation Format

Share Document