Scanning electron microscopy of stromal cells of human placental villi throughout pregnancy

1984 ◽  
Vol 235 (3) ◽  
Author(s):  
C. Martinoli ◽  
M. Castellucci ◽  
D. Zaccheo ◽  
P. Kaufmann
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Stromal Cells ◽  
Placental Villi ◽  
Scanning Electron

High resolution three dimensional imaging of placental villi using serial block face scanning electron microscopy

Placenta ◽  
2016 ◽  
Vol 45 ◽  
pp. 84
Author(s):  
Eleni Palaiologou ◽  
Wendy Chiu ◽  
Rodolfo Ribeiro de Souza ◽  
Patricia Goggin ◽  
Emma Lofthouse ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Face Scanning ◽  
Placental Villi ◽  
Block Face ◽  
Scanning Electron

3D imaging of full-term human placental villi by serial block face-scanning electron microscopy

Placenta ◽  
2017 ◽  
Vol 59 ◽  
pp. 175-176
Author(s):  
Haruhiko Shimada ◽  
Yuuki Yamaguchi ◽  
Takami Takizawa ◽  
Hironori Takahashi ◽  
Akihid Ohkuchi ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
3D Imaging ◽  
Full Term ◽  
Face Scanning ◽  
Placental Villi ◽  
Block Face ◽  
Scanning Electron

Morphological differences between placental villi and hydatidiform villi in scanning electron microscopy

Placenta ◽  
1993 ◽  
Vol 14 (4) ◽  
pp. A13
Author(s):  
L. Danihel ◽  
Š. Polák ◽  
P. Šuška ◽  
M. Korbel ◽  
J. Vojtassak
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Placental Villi ◽  
Morphological Differences ◽  
Scanning Electron

Pathogenesis of Human Hair Defects

1974 ◽  
Vol 32 ◽  
pp. 12-13
Author(s):  
P.S. Porter ◽  
T. Aoyagi ◽  
R. Matta
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Hair ◽  
Standard Techniques ◽  
Scanning Electron

Using standard techniques of scanning electron microscopy (SEM), over 1000 human hair defects have been studied. In several of the defects, the pathogenesis of the abnormality has been clarified using these techniques. It is the purpose of this paper to present several distinct morphologic abnormalities of hair and to discuss their pathogenesis as elucidated through techniques of scanning electron microscopy.

Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

1977 ◽  
Vol 35 ◽  
pp. 638-639
Author(s):  
P.J. Dailey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Salivary Glands ◽  
Secretory Vesicles ◽  
The Past ◽  
Transmission Electron ◽  
Means Of Transmission ◽  
Gromphadorhina Portentosa ◽  
Scanning Electron ◽  
Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

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