Analysis of DNA from a Beta procumbens chromosome fragment in sugar beet carrying a gene for nematode resistance

1990 ◽  
Vol 79 (5) ◽  
pp. 663-672 ◽  
Author(s):  
C. Jung ◽  
M. Kleine ◽  
E. Fischer ◽  
R. G. Herrmann
Keyword(s):  
Sugar Beet ◽  
Nematode Resistance ◽  
Chromosome Fragment ◽  
Beta Procumbens

Molecular Analysis of a Translocation in Sugar Beet Carrying a Gene for Nematode Resistance from Beta procumbens

1994 ◽  
Vol 31 (1&2) ◽  
pp. 27-42 ◽  
Author(s):  
Christian Jung ◽  
Reinhold G. Herrman ◽  
Christian Eibl ◽  
Michael Kleine
Keyword(s):  
Sugar Beet ◽  
Molecular Analysis ◽  
Nematode Resistance ◽  
Beta Procumbens

Breeding and usage of sugar beet cultivars and hybrids resistant to sugar beet nematode Heterodera schachtii

2015 ◽  
Vol 2 (1) ◽  
pp. 12-22 ◽  
Author(s):  
L. Pylypenko ◽  
K. Kalatur
Keyword(s):  
Sugar Beet ◽  
Sugar Content ◽  
Production Capacity ◽  
Nematode Resistance ◽  
Control Measures ◽  
Heterodera Schachtii ◽  
Yield Reduction ◽  
Agricultural Practice ◽  
Germplasm Exchange ◽  
Good Agricultural Practice

Heterodera schachtii Schmidt, 1871 is one of the most economically important pests of sugar beet (Beta vulgaris L.) worldwide. It is also widespread in most sugar beet growing regions in Ukraine causing serious yield reduction and decreasing sugar content of sugar beet in infested fi elds. An advanced parasitic strategy of H. schachtii is employed to support nematode growth, reproduction and harmfulness. In intensive agriculture systems the nematode control measures heavily rely on nematicides and good agricultural practice (crop rota- tion in the fi rst place). But alternative strategies based on nematode resistant sugar beet cultivars and hybrids are required as none of nematicides approved for the open fi eld application are registered in Ukraine. Here we review the achievements and problems of breeding process for H. schachtii resistance and provide the results of national traditional breeding program. Since the beginning of 1980s fi ve sugar beet cultivars (Verchnyatskyi 103, Yaltuschkivska 30, Bilotcerkivska 45, BTs-40 and Yuvileynyi) and seventeen lines partly resistant or toler- ant to H. schachtii have been obtained throughout targeted crossing and progenies assessment in the infested fi elds. The further directions for better utilization of genetic sources for nematode resistance presented in na- tional gene bank collection are emphasized. There is a need for more accurate identifi cation of resistance genes, broader application of reliable molecular markers (suitable for marker-assisted selection of nematode resistant plants in the breeding process) and methods for genetic transformation of plants. Crop cash value and national production capacity should drive the cooperation in this fi eld. Knowledge as well as germplasm exchange are thereby welcomed that can benefi t breeding progress at national and international level.

Nematode Resistance Derived from Wild Beet and its Meiotic Stability in Sugar Beet

1987 ◽  
Vol 99 (1) ◽  
pp. 56-64 ◽  
Author(s):  
Andrea Brandes ◽  
C. Jungand ◽  
G. Wricke
Keyword(s):  
Sugar Beet ◽  
Nematode Resistance ◽  
Meiotic Stability ◽  
Wild Beet

A complete physical map of a wild beet (Beta procumbens) translocation in sugar beet

2006 ◽  
Vol 275 (5) ◽  
pp. 504-511 ◽  
Author(s):  
Daniela Schulte ◽  
Daguang Cai ◽  
Michael Kleine ◽  
Longjiang Fan ◽  
Sheng Wang ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Physical Map ◽  
Beta Procumbens ◽  
Wild Beet

Identification and Validation of a SNP Marker Linked to the Gene HsBvm-1 for Nematode Resistance in Sugar Beet

2014 ◽  
Vol 33 (3) ◽  
pp. 474-479 ◽  
Author(s):  
Piergiorgio Stevanato ◽  
Daniele Trebbi ◽  
Lee Panella ◽  
Kelley Richardson ◽  
Chiara Broccanello ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Nematode Resistance ◽  
Snp Marker

Construction and characterization of a BAC library for the molecular dissection of a single wild beet centromere and sugar beet (Beta vulgaris) genome analysis

Genome ◽  
2001 ◽  
Vol 44 (5) ◽  
pp. 846-855 ◽  
Author(s):  
Frank Gindullis ◽  
Daryna Dechyeva ◽  
Thomas Schmidt
Keyword(s):  
Sugar Beet ◽  
Beta Vulgaris ◽  
Bac Library ◽  
Artificial Chromosome ◽  
Average Insert Size ◽  
Insert Size ◽  
Single Chromosome ◽  
Beta Procumbens ◽  
Wild Beet

We have constructed a sugar beet bacterial artificial chromosome (BAC) library of the chromosome mutant PRO1. This Beta vulgaris mutant carries a single chromosome fragment of 6-9 Mbp that is derived from the wild beet Beta procumbens and is transmitted efficiently in meiosis and mitosis. The library consists of 50 304 clones, with an average insert size of 125 kb. Filter hybridizations revealed that approximately 3.1% of the clones contain mitochondrial or chloroplast DNA. Based on a haploid genome size of 758 Mbp, the library represents eight genome equivalents. Thus, there is a greater than 99.96% probability that any sequence of the PRO1 genome can be found in the library. Approximately 0.2% of the clones hybridized with centromeric sequences of the PRO1 minichromosome. Using the identified BAC clones in fluorescence in situ hybridization experiments with PRO1 and B. procumbens chromosome spreads, their wild-beet origin and centromeric localization were demonstrated. Comparative Southern hybridization of pulsed-field separated PRO1 DNA and BAC inserts indicate that the centromeric region of the minichromosome is represented by overlapping clones in the library. Therefore, the PRO1 BAC library provides a useful tool for the characterization of a single plant centromere and is a valuable resource for sugar beet genome analysis.Key words: Beta vulgaris, BAC library, Beta procumbens minichromosome, centromere, FISH.

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