Production of wheat-barley recombinant chromosomes through induced homoeologous pairing

1992 ◽  
Vol 83 (4) ◽  
pp. 489-494 ◽  
Author(s):  
A. K. M. R. Islam ◽  
K. W. Shepherd
Keyword(s):  
Homoeologous Pairing ◽  
Induced Homoeologous Pairing

Characterization of interspecific hybrids of Triticum aestivum x Aegilops sp. without 5B chromosome for induced homoeologous pairing

2015 ◽  
Vol 25 (1) ◽  
pp. 117-120 ◽  
Author(s):  
Imran Sheikh ◽  
Prachi Sharma ◽  
Shailender Kumar Verma ◽  
Satish Kumar ◽  
Sachin Malik ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Interspecific Hybrids ◽  
Homoeologous Pairing ◽  
Induced Homoeologous Pairing

Analysis of induced homoeologous pairing in a ph mutant wheat X rye hybrid

1977 ◽  
Vol 68 (4) ◽  
pp. 207-209 ◽  
Author(s):  
HARCHARAN S. DHALIWAL ◽  
BIKRAM S. GILL ◽  
J. GILES WAINES
Keyword(s):  
Homoeologous Pairing ◽  
Induced Homoeologous Pairing

Induced Homoeologous Pairing for Transfer of Useful Variability for High Grain Fe and Zn from Aegilops kotschyi into Wheat

2016 ◽  
Vol 34 (6) ◽  
pp. 1083-1094 ◽  
Author(s):  
Shailender Kumar Verma ◽  
Satish Kumar ◽  
Imran Sheikh ◽  
Prachi Sharma ◽  
Priyanka Mathpal ◽  
...  
Keyword(s):  
Homoeologous Pairing ◽  
Induced Homoeologous Pairing

Synthesis and cytological characterization of trigeneric hybrids of durum wheat with and without Ph1

Genome ◽  
2004 ◽  
Vol 47 (6) ◽  
pp. 1173-1181 ◽  
Author(s):  
Prem P Jauhar ◽  
M Doğramaci ◽  
T S Peterson
Keyword(s):  
In Situ Hybridization ◽  
Chromosome Pairing ◽  
Genetic Recombination ◽  
Genomic In Situ Hybridization ◽  
Homoeologous Pairing ◽  
Alien Gene Transfer ◽  
Chromosome 5B ◽  
Alien Gene ◽  
Trigeneric Hybrids

Wild grasses in the tribe Triticeae, some in the primary or secondary gene pool of wheat, are excellent reservoirs of genes for superior agronomic traits, including resistance to various diseases. Thus, the diploid wheatgrasses Thinopyrum bessarabicum (Savul. and Rayss) Á. Löve (2n = 2x = 14; JJ genome) and Lophopyrum elongatum (Host) Á. Löve (2n = 2x = 14; EE genome) are important sources of genes for disease resistance, e.g., Fusarium head blight resistance that may be transferred to wheat. By crossing fertile amphidiploids (2n = 4x = 28; JJEE) developed from F1 hybrids of the 2 diploid species with appropriate genetic stocks of durum wheat, we synthesized trigeneric hybrids (2n = 4x = 28; ABJE) incorporating both the J and E genomes of the grass species with the durum genomes A and B. Trigeneric hybrids with and without the homoeologous-pairing suppressor gene, Ph1, were produced. In the absence of Ph1, the chances of genetic recombination between chromosomes of the 2 useful grass genomes (JE) and those of the durum genomes (AB) would be enhanced. Meiotic chromosome pairing was studied using both conventional staining and fluorescent genomic in situ hybridization (fl-GISH). As expected, the Ph1-intergeneric hybrids showed low chromosome pairing (23.86% of the complement), whereas the trigenerics with ph1b (49.49%) and those with their chromosome 5B replaced by 5D (49.09%) showed much higher pairing. The absence of Ph1 allowed pairing and, hence, genetic recombination between homoeologous chromosomes. Fl-GISH analysis afforded an excellent tool for studying the specificity of chromosome pairing: wheat with grass, wheat with wheat, or grass with grass. In the trigeneric hybrids that lacked chromosome 5B, and hence lacked the Ph1 gene, the wheat–grass pairing was elevated, i.e., 2.6 chiasmata per cell, a welcome feature from the breeding standpoint. Using Langdon 5D(5B) disomic substitution for making trigeneric hybrids should promote homoeologous pairing between durum and grass chromosomes and hence accelerate alien gene transfer into the durum genomes.Key words: alien gene transfer, chiasma (xma) frequency, chromosome pairing, fluorescent genomic in situ hybridization (fl-GISH), homoeologous-pairing regulator, specificity of chromosome pairing, wheatgrass.

The Effect of Homoeologous Meiotic Pairing in Tetraploid Hordeum bulbosum L. × H. vulgare L. Hybrids on Alien Introgressions in Offspring

2016 ◽  
Vol 150 (2) ◽  
pp. 139-149 ◽  
Author(s):  
Margret Scholz ◽  
Galina Pendinen
Keyword(s):  
Interspecific Hybrids ◽  
In Situ Hybridisation ◽  
Basic Material ◽  
Meiotic Pairing ◽  
Hordeum Bulbosum ◽  
Homoeologous Pairing ◽  
Introgression Breeding ◽  
The Individual ◽  
Pairing Behaviour

The pairing behaviour of the individual chromosome arms of Hordeum vulgare (Hv) with their homoeologous arms of H. bulbosum (Hb) at metaphase I of meiosis in tetraploid Hb × Hv hybrids and the frequencies of recombined Hv chromosome arms in selfed offspring were studied on differentially visualized chromosomes after fluorescent in situ hybridisation. The frequencies of paired Hv-Hb arms in the F2 and F3 hybrids were correlated with the frequencies of recombined Hv chromosomes in progenies. Self-generation of hybrids, the number of Hv and Hb chromosomes, and the number of recombined Hv chromosomes of the hybrids strongly influenced the Hv-Hb pairing frequency in meiosis. Within the offspring of F2 and F3 hybrids both Hv plants and hybrids were detected. In contrast, all progenies of the F4 hybrid were hybrids which exhibited centromere misdivisions. The highest frequencies of homoeologous pairing in hybrids and most recombinants were obtained for the barley chromosome 1HL. Recombinants for 4HL, 5HS, 6HS, and 7HS were rarely found. Meiotic pairing and recombinants involving chromosome 1HS were never observed. The results of this study demonstrate that fertile tetraploid interspecific hybrids with a high intergenomic pairing at meiosis are valuable basic material for introgression breeding in barley.

scholarly journals GENOME SIZE IN THREE SPECIES OF Glandularia AND THEIR HYBRIDS

2019 ◽  
Vol 30 (2) ◽  
pp. 47-54
Author(s):  
M.R. Ferrari ◽  
E.J. Greizerstein ◽  
L. Poggio
Keyword(s):  
Genome Size ◽  
Dna Content ◽  
High Frequency ◽  
Parental Species ◽  
The Other ◽  
F1 Hybrids ◽  
Homoeologous Pairing ◽  
The Relationship ◽  
Species Being ◽  
Homoeologous Chromosomes

In this work the relationship between genome size of Glandularia species and the meiotic configurations found in their hybrids are discussed. Glandularia incisa (Hook.) Tronc., growing in two localities of Corrientes and Córdoba provinces, Argentina, with different ecological conditions, showed inter-population variability of the 2C-value. The DNA content found in the Corrientes locality (2.41 pg) was higher than that obtained in the Córdoba locality (2.09 pg) which has more stressful environmental conditions than the former. These values are statistically different from those that were found in Glandularia pulchella (Sweet) Tronc. from Corrientes (1.43 pg) and in Glandularia perakii Cov. et Schn from Córdoba (1.47 pg). The DNA content of the diploid F1 hybrids, G. pulchella × G. incisa and G. perakii × G. incisa, differed statistically from the DNA content of the parental species, being intermediate between them. Differences in the frequency of pairing of homoeologous chromosomes were observed in the hybrids; these differences cannot be explained by differences in genome size since hybrids with similar DNA content differ significantly in their meiotic behavior. On the other hand, the differences in the DNA content between the parental species justify the presence of a high frequency of heteromorphic open and closed bivalents and univalents with different size in the hybrids. Key words: Intra-specific DNA content variability, homoeologous pairing, heteromorphic bivalents

A WHEAT MUTATION CONDITIONING AN INTERMEDIATE LEVEL OF HOMOEOLOGOUS CHROMOSOME PAIRING

1982 ◽  
Vol 24 (6) ◽  
pp. 715-719 ◽  
Author(s):  
E. R. Sears
Keyword(s):  
Chromosome Pairing ◽  
Triticum Aestivum L ◽  
Terminal Segment ◽  
Intermediate Level ◽  
Homoeologous Pairing ◽  
Homoeologous Chromosome ◽  
X Ray ◽  
Homoeologous Chromosome Pairing ◽  
Homozygous Line ◽  
A Minor

An X-ray-induced mutation in common wheat (Triticum aestivum L.), designated ph2, conditions an intermediate level of homoeologous chromosome pairing in hybrids with Triticum kotschyi var. variabilis. The number of chromosomes paired averaged 9.2 per sporocyte, compared with 2.0 in the control and 27.9 in the same hybrid involving ph1b, an apparent deficiency for Ph1 obtained in the same mutation experiment. The ph2 mutation is located on chromosome 3D and is believed to be a deficiency for a terminal segment of the short arm that includes the locus of Ph2, a minor suppressor of homoeologous pairing. Although no pairing of the ph2-carrying chromosome with telosome 3DS was observed, the mutation is clearly not a deficiency for the entire arm. It has little effect on pairing in wheat itself. Male transmission of the mutation is approximately normal, and fertility, while reduced, is sufficient for easy maintenance of the homozygous line.

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